Surveillance of bacterial colonisation on contact surfaces in different medical wards

Abstract

This study was conducted to determine the bacterial colonization of some bacterial groups, including extended-spectrum β-lactamase (ESBLs) producers and methicillin-resistant Staphylococcus aureus (MRSA), on surfaces of the equipment and instruments in patient rooms and other workspaces in three different medical wards. The number of microorganisms on swabs was determined with the colony count method on selective microbiological mediums. The aerobic mesophylic microorganisms were found in 73.5 % out of 102 samples, with the average and maximum values of 2.6 × 102 and 4.6 × 103 colony forming units (CFU) 100 cm-2, respectively. Members of the family Enterobacteriaceae, coagulase positive staphylococci, coagulase-negative staphylococci, and enterococci were detected in 23.4, 31.4, 53.2, and 2.9 % of samples, respectively. The differences in bacterial counts on the surfaces of the psychiatric, oncology, and paediatric wards were statistically significant (P<0.001). About 40 % out of 19 isolates from the family Enterobacteriaceae showed multiple resistance to three or more different groups of tested antibiotics, while ESBL was confirmed for only one strain. Staphylococci isolates were mostly resistant to penicillin. MRSA was confirmed in 5.2 % of the tested S. aureus isolates. Greater attention should be paid to cleaning and the appropriate choice of disinfectants, especially in the psychiatric ward. Employees should be informed about the prevention of the spreading of nosocomial infections. Routine application of rapid methods for hygiene control of surfaces is highly recommended.

Environmental bacterial and fungal contamination in high touch surfaces and indoor air of a paediatric intensive care unit in Maputo Central Hospital, Mozambique in 2018

“…We observed low susceptibility to AMP, SXT, P and Ox for the isolated pathogens, as reported in previous studies [ 33 , 35 , 36 ].…”

Surface Disinfections: Present and Future

“…From surfaces, pathogens may infect patients by direct contact or indirectly, by means of the hands of medicals and healthcare workers. Thus cleaning and surface disinfection are very important in order to limit their transfer and reduce their diffusion [28]. The efficacy of cleaning practices can be affected by many factors, which can compromise the disinfectant action itself.…”

Comparison of nylon-flocked swab and cellulose sponge methods for carbapenem-resistant Enterobacteriaceae and gram-negative organism recovery from high-touch surfaces in patient rooms

“…We are not aware of any other studies looking at all Gram-negative organism recovery, however MDR Gram-negative organism recovery can range from 1.8 to 30% of surfaces. 9,10 Shams et al found 34% of high touch surfaces contaminated with MDROs post-daily cleaning, although mostly with Gram-positive organisms. 6 …”