Environmental bacterial and fungal contamination in high touch surfaces and indoor air of a paediatric intensive care unit in Maputo Central Hospital, Mozambique in 2018

Maphossa, Vânia; Langa, José Carlos; Simbine, Samuel; Maússe, Fabião Edmundo; Kenga, Darlene; Relvas, Ventura; Chicamba, Valeria; Manjate, Alice; Sacarlal, Jahit

doi:10.1016/j.infpip.2022.100250

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…The study ndings revealed that Coagulase-negative Staphylococcus, a nonpathogenic resident ora constituent, was the most common gram-positive bacteria isolated from inanimate surfaces and medical equipment, with a prevalence of 46.91% in the adult intensive care unit and 34% in the neonatal intensive care unit. This nding is in agreement with a study assessing surface cleaning and disinfection in the neonatal intensive care unit, which showed that coagulase-negative Staphylococcus was the predominant isolate(8), and with another study performed in 2018 in Mozambique on environmental bacterial and fungal contamination in high-touch surfaces in the pediatric ICU (9). Although several studies have reported Staphylococcus aureus, which is a common cause of nosocomial infections, as the most isolated bacterium (4), Staphylococcus aureus was not isolated in this study.…”

Section: Discussionsupporting

confidence: 92%

Drug-resistant bacteria were found to contaminate inanimate surfaces and equipment in the Intensive Care Unit at Kawempe National Referral Hospital.

Aston,

Nalwanga,

Magulye

et al. 2024

Preprint

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Background Hospital inanimate surfaces and equipment in intensive care units (ICUs) provide a worrisome reservoir for drug-resistant microorganisms, which cause healthcare-associated infections (HCAIs) in mostly developing countries. This study aimed to determine the prevalence and antimicrobial susceptibility profiles of bacteria isolated from inanimate surfaces in the intensive care unit at Kawempe National Referral Hospital (KNRH). Methods A cross-sectional study was conducted from December 2022 to April 2023 in the ICU at KNRH. Random sampling was used to swab 15 pieces of selected equipment on points of contact with the user and points of contact with the patient and inanimate surfaces. The samples were analyzed for bacterial growth using standard microbiological methods. The antimicrobial susceptibility of the identified bacterial isolates was tested against commonly used antibiotics. All the data were analyzed in STATA version 12. Results A total of 76.2% (76/101) of the samples were positive for bacterial growth. The most common bacterial contaminants of inanimate surfaces and equipment in the adult ICU and neonatal ICU were CNSs (46.9% vs. 34.64%). All the gram-negative bacteria isolated were resistant to ceftazidime and ampicillin. Among the Enterobacterales strains tested for ESBL production, 62.5% were ESBL positive Conclusion The intensivecare unit (ICU) environment is known to act as areservoir of potentially pathogenic microorganisms. Inanimate surfaces and medical equipment were found to be highly contaminated,and the predominant bacteria isolated from inanimate surfaces and medical equipment were coagulase-negative staphylococci, Acinetobacter species, Bacillus species, Micrococcus species, A. baumannii and K. pneumoniae. These isolates can potentially cause health care-associated infections. Therefore, routine surveillance of hospital inanimate surfaces and medical equipment is needed for timely intervention to reduce environmental contamination by resistant bacterial species and prevent hospital infection.

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Section: Discussionsupporting

confidence: 92%

Drug-resistant bacteria were found to contaminate inanimate surfaces and equipment in the Intensive Care Unit at Kawempe National Referral Hospital.

Aston,

Nalwanga,

Magulye

et al. 2024

Preprint

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“…Serratia odorifera is rarely implicated in clinical infections but is often associated with invasive human infections [73]. Some strains that have been isolated in hospitals have shown multidrug resistance to antibiotics [74]. Their presence in fish thus constitutes a significant health risk because they can be transmitted to the consumer through contaminated food [75].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Molecular Characterization of <i>Staphylococcaceae</i> and <i>Enterobacteriaceae</i> Species Isolated from Smoked, Dried, and Braised Fish Marketed in Ouagadougou

Ouedraogo¹,

Traoré²,

Ouédraogo³

et al. 2023

AiM

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Background: To preserve its nutritional properties, fish must have good sanitary quality. The objective of this study was to investigate some pathogens contaminating smoked, dried, and braised fish marketed in Ouagadougou.Methodology: Potential pathogens of Enterobacteriaceae and Staphylococcus were screened in eight (8) species of processed fish. The investigation of the germs was carried out following the normative methods of microbiology. The identities of the strains were determined by API 20 E (BioMerieux S.A., France) and API STAPH (BioMerieux S.A., France) kits for Enterobacteriaceae and Staphylococcus species respectively. The uidA gene profile in Escherichia coli isolates was determined by simplex PCR. The identity of Staphylococcus aureus was confirmed by amplification of specific 23S rDNA regions and nuc gene profile with PCR. Results: A total of 235 fish samples were analyzed. A diversity of Enterobacteriaceae and Staphylococcus was detected. Twenty species of Enterobacteriaceae were identified among which, the most frequent were Escherichia coli, Salmonella sp, Raoultella ornithinolytica and Serratia odorifera, respectively in 22.6%, 4.3%, 28.9%, 17.4% of the samples analyzed. However, eleven species of Staphylococcus were identified among How to cite this paper: Ouédraogo, A.,

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A comprehensive review of microbial contamination in the indoor environment: sources, sampling, health risks, and mitigation strategies

Chawla,

Anand,

Garg

et al. 2023

Front. Public Health

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The quality of the indoor environment significantly impacts human health and productivity, especially given the amount of time individuals spend indoors globally. While chemical pollutants have been a focus of indoor air quality research, microbial contaminants also have a significant bearing on indoor air quality. This review provides a comprehensive overview of microbial contamination in built environments, covering sources, sampling strategies, and analysis methods. Microbial contamination has various origins, including human occupants, pets, and the outdoor environment. Sampling strategies for indoor microbial contamination include air, surface, and dust sampling, and various analysis methods are used to assess microbial diversity and complexity in indoor environments. The review also discusses the health risks associated with microbial contaminants, including bacteria, fungi, and viruses, and their products in indoor air, highlighting the need for evidence-based studies that can relate to specific health conditions. The importance of indoor air quality is emphasized from the perspective of the COVID-19 pandemic. A section of the review highlights the knowledge gap related to microbiological burden in indoor environments in developing countries, using India as a representative example. Finally, potential mitigation strategies to improve microbiological indoor air quality are briefly reviewed.

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Environmental bacterial and fungal contamination in high touch surfaces and indoor air of a paediatric intensive care unit in Maputo Central Hospital, Mozambique in 2018

Cited by 3 publications

References 31 publications

Drug-resistant bacteria were found to contaminate inanimate surfaces and equipment in the Intensive Care Unit at Kawempe National Referral Hospital.

Drug-resistant bacteria were found to contaminate inanimate surfaces and equipment in the Intensive Care Unit at Kawempe National Referral Hospital.

Phenotypic and Molecular Characterization of <i>Staphylococcaceae</i> and <i>Enterobacteriaceae</i> Species Isolated from Smoked, Dried, and Braised Fish Marketed in Ouagadougou

A comprehensive review of microbial contamination in the indoor environment: sources, sampling, health risks, and mitigation strategies

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Environmental bacterial and fungal contamination in high touch surfaces and indoor air of a paediatric intensive care unit in Maputo Central Hospital, Mozambique in 2018

Cited by 3 publications

References 31 publications

Drug-resistant bacteria were found to contaminate inanimate surfaces and equipment in the Intensive Care Unit at Kawempe National Referral Hospital.

Drug-resistant bacteria were found to contaminate inanimate surfaces and equipment in the Intensive Care Unit at Kawempe National Referral Hospital.

Phenotypic and Molecular Characterization of &lt;i&gt;Staphylococcaceae&lt;/i&gt; and &lt;i&gt;Enterobacteriaceae&lt;/i&gt; Species Isolated from Smoked, Dried, and Braised Fish Marketed in Ouagadougou

A comprehensive review of microbial contamination in the indoor environment: sources, sampling, health risks, and mitigation strategies

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Phenotypic and Molecular Characterization of <i>Staphylococcaceae</i> and <i>Enterobacteriaceae</i> Species Isolated from Smoked, Dried, and Braised Fish Marketed in Ouagadougou