Surgical Technique of Orthotopic Liver Transplantation in Rats: The Kamada Technique and a New Splint Technique for Hepatic Artery Reconstruction

Ishii, Eiichi; Shimizu, Akira; Takahashi, Mikiko; Terasaki, Mika; Κunugi, Shinobu; Nagasaka, Shinya; Terasaki, Yasuhiro; Ohashi, Ryuji; Masuda, Yuki; Fukuda, Yuh

doi:10.1272/jnms.80.4

Cited by 10 publications

(11 citation statements)

References 55 publications

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“…In 1975, Lee simplified his model, omitting the portocaval shunt as well as graft rearterialization [4]. Based on Lee's pioneering work, several new models and modifications were introduced during the last 4 decades [13,14]. …”

Section: Technical Evolution Of Rat Olt and P-olt Modelsmentioning

confidence: 99%

“…A more detailed description of the basic surgical techniques of rat OLT and the dozens of modifications is beyond the scope of this review; nevertheless, extensive data on this topic can be found in previous reviews [13,14]. …”

Section: Technical Evolution Of Rat Olt and P-olt Modelsmentioning

confidence: 99%

“…Besides these well-designed multistep training programs, further reports are available in the literature that provide a detailed, pragmatic surgical guide for researchers who are aiming to master this model [14,20,37,47]. …”

Section: Microsurgical Trainingmentioning

confidence: 99%

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Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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Background: Due to a worldwide shortage of donor organs for liver transplantation, alternative approaches, such as split and living donor liver transplantations, were introduced to increase the donor pool and reduce mortality on liver transplant waiting lists. Numerous details concerning the mechanisms and pathophysiology of liver regeneration, small-for-size syndrome, rejection, and tolerance in partial liver transplantation facilitated the development of various animal models. The high number of preclinical animal studies contributed enormously to our understanding of many clinical aspects of living donor and partial liver transplantations. Summary: Microsurgical rat models of partial orthotopic liver transplantation are well established and widely used. Nevertheless, several issues regarding this procedure are controversial, not clarified, or not yet properly standardized (graft rearterialization, size reduction techniques, etc.). The major aim of this literature review is to give the reader a current overview of rat orthotopic liver transplantation models with a special focus on partial liver transplantation. The aspects of model evolution, microsurgical training, and different technical problems are analyzed and discussed in detail. Our further aim in this paper is to elaborate a detailed publication guide in order to improve the quality of reporting in the field of rat liver transplantation according to the ARRIVE guidelines and the 3R principle. Key Messages: Partial orthotopic liver transplantation in rats is an indispensable, reliable, and cost-efficient model for transplantation research. A certain consensus on different technical issues and a significant improvement in scientific reporting are essential to improve transparency and comparability in this field as well as to foster refinement.

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Section: Technical Evolution Of Rat Olt and P-olt Modelsmentioning

confidence: 99%

Section: Technical Evolution Of Rat Olt and P-olt Modelsmentioning

confidence: 99%

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Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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“…For the ROLT, there was a consensus that the anastomosis of the IHVC and the PV were achieved by the cuff technique [23], [24], [25], [26], [27]. Although there are three different of methods, the reconstruction of the SHVC remains the most difficult part of ROLT.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Suprahepatic Vena Cava Reconstruction Methods on the Hemodynamics of Rats after Liver Transplantation

Wang

et al. 2013

PLoS ONE

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BackgroundThere are few studies on the hemodynamic changes after orthotopic liver transplantation in rats. In this study, we aimed to evaluate the effect of different suprahepatic vena cava (SHVC) reconstruction methods on the hemodynamics of rats after liver transplantation.Materials and MethodsThree rat liver transplantation groups were created according to the SHVC reconstruction method: Kamada’s two-cuff technique, a modified veno-lined stent technique, and Harihara’s three-cuff technique. Ten rats of similar weight were grouped as the control. Anatomical, ultrasonic, and hemodynamic parameters and the microcirculation of the liver were measured after transplantation. The detailed operation time, operative complications, and animal survival were recorded.ResultsAll the recipients showed portal hypertension one month after transplantation. The portal hypertension in the group with the modified veno-lined stent technique was the most severe. The value measured with real-time elastography was significantly higher in the recipients using the modified veno-lined stent technique than in the other two groups (P<0.01). There was no difference in the graft microcirculation after reperfusion among the three groups. The survival rate of the three groups displayed no difference, but the modified veno-lined stent technique led to more venous complications than the other two techniques.ConclusionsThe hemodynamics after liver transplantation in rats is determined not only by the cuff used for portal vein reconstruction but also by the cuff or stent for the SHVC. Some SHVC reconstruction methods, such as the modified veno-lined stent technique, Miyata’s or Settaf’s three-cuff techniques, significantly affect the hemodynamics.

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“…DA rat liver grafts transplanted into Lewis rats without immunosuppression [8]. Ten-week-old DA and Lewis rats weighting 200 g at the time of surgery were used for both donors and recipients.…”

Section: Methodsmentioning

confidence: 99%

Renal Inflammatory Changes in Acute Hepatic Failure-Associated Acute Kidney Injury

Shimizu

Ishii²,

Masuda³

et al. 2013

Am J Nephrol

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Background/Aims: Acute kidney injury (AKI) is a common complication in advanced liver dysfunction. Our aim is to clarify the mechanisms of acute hepatic failure (AHF)-associated AKI. Methods: We examined the mechanisms of AHF-associated AKI, which is characterized by AKI in AHF and hyperbilirubinemia, following DA-to-Lewis rat liver transplantation. Results: During the progression of AHF and hyperbilirubinemia in liver graft rejection, AHF-associated AKI gradually developed by day 11. Degeneration and apoptotic cells were apparent in tubular epithelial cells with bile pigment accumulation and mitochondrial degeneration. Injury of peritubular capillaries (PTCs) was also noted with apoptotic endothelial cells, decreased expression of endothelial nitric oxide synthase, accumulation of α-smooth muscle actin+ pericytes and/or myofibroblasts, and inflammation. Angiogenic factors including vascular endothelial growth factor, angiopoietin-1, and angiopoietin-2 in the cortex were decreased on day 11. In addition, a marked reduction in the velocity of red blood cells in PTCs was evident in vivo. Conclusions: AHF-associated AKI seems to be mediated by renal tubular epithelial cell injury with bile pigment accumulation, impaired microcirculation caused by PTC endothelial cell injury with depletion of endothelial nitric oxide synthase and angiogenic factors, and by a decrease in RBC velocity and renal inflammation. Multiple mechanisms including tubular and PTC injuries and renal inflammation may be involved in the development of AHF-associated AKI.

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Surgical Technique of Orthotopic Liver Transplantation in Rats: The Kamada Technique and a New Splint Technique for Hepatic Artery Reconstruction

Cited by 10 publications

References 55 publications

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Effect of Different Suprahepatic Vena Cava Reconstruction Methods on the Hemodynamics of Rats after Liver Transplantation

Renal Inflammatory Changes in Acute Hepatic Failure-Associated Acute Kidney Injury

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