Surgical Removal of Endometrioma Decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) Expression in the Eutopic Endometrium During the Implantation Window

Abstract

We aimed to investigate whether the surgical removal of endometrioma alters the nuclear factor-kappa B1 (NF-kB1; p50/105) and NF-kB p65 (Rel A) expression in the eutopic endometrium of infertile women with endometrioma before and after laparoscopic removal of the ovarian endometrioma during the mid-secretory phase. Infertile women with endometrioma (n = 15) were enrolled. Infertile patients with nonendometriotic ovarian cyst (n = 10) and healthy fertile women (n = 10) were recruited as controls. Endometrial samples were obtained before and 3 months after the laparoscopic cystectomy. The NF-kB1 (p50/105) levels were analyzed by enzyme-linked immunosorbent assay (ELISA) in the endometrium of all groups before and after laparoscopic ovarian cystectomy during implantation window. Expression of NF-kB1 (p50/105) in eutopic endometrium was significantly higher in infertile women with endometrioma compared to nonendometriotic cyst and fertile controls (P < .05). Laparoscopic cystectomy resulted in a significant decrease in NF-kB1 expression in women with endometrioma. The NF-kB p65 (Rel A) immunoreactivity of eutopic endometrium decreased significantly subsequent to the surgical removal of the endometrioma. In conclusion, increased endometrial NF-kB expression may contribute to endometriosis-associated infertility.

Laparoscopic endometrioma resection increases peri-implantation endometrial HOXA-10 and HOXA-11 mRNA expression

“…In addition, chronic inflammation, which is a main component of endometriosis, can lead to epigenetic changes (65). A previous study by our group demonstrated that presence of endometrioma increased nuclear factor kB-related endometrial inflammation and that surgical removal of endometrioma decreased this inflammation (11). Taken together, we can suggest that interruption of pathologic inflammation in endometrium by laparoscopic removal of endometrioma may inhibit hypermethylation of HOXA-10 and HOXA-11 genes and thus increase their expression.…”

“…Werbrouck et al (59) demonstrated no significant difference between unexplained infertile patients and surgically treated minimal to mild endometriosis after controlled ovarian hyperstimulation/intrauterine insemination treatment. Recently, our group has demonstrated that laparoscopic stripping of endometrioma decreases the endometrial expression of inflammatory cytokines (11).…”

“…Although the receptivity of eutopic endometrium of patients having ovarian endometrioma might not be altered during the window of implantation (WOI) (9), the underlying mechanisms of endometriomaassociated infertility remain elusive. However, recent human and other animal studies with the use of different technologies have revealed differences in the expression of several genes and inflammatory cytokines in eutopic endometrium of women with endometriosis or endometrioma (2,10,11). Genetic research of the endometrium in women with endometriosis showed abnormal expression of putative implantation markers, such as homeobox genes, avb3 integrins, and leukemia inhibitory factor (LIF) (12)(13)(14)(15)(16).…”

“…To date, it remains to be clarified whether surgical treatment of endometrioma improves reproductive outcome (11,42). There are no controlled studies investigating the effect of surgical removal of endometrioma on the expression levels of homeobox genes, integrins, and LIF in the eutopic endometrium.…”

Cardioprotective effects of anisodamine against myocardial ischemia/reperfusion injury through the inhibition of oxidative stress, inflammation and apoptosis

“…During reperfusion, the generation of reactive oxygen species (ROS) is enhanced (7). ROS can destroy the structure of cell membranes through lipid peroxidation (8), thus causing an increase in membrane permeability, resulting in cellular edema. The structure of the mitochondrial membrane is also compromised, thus damaging mitochondrial function and impairing energy production, further aggravating cellular injury.…”