Surgical and Molecular Evaluation of Pediatric Hydatid Cyst Cases in Eastern Turkey

Abstract

Cystic echinococcosis (CE) caused by Echinococcus granulosus is a major public health problem worldwide, including Turkey. The aim of the current study was to identify the strains and to estimate the potential risk factors of E. granulosus in operated pediatric cases in eastern Turkey. Ten pediatric patients (7 boys and 3 girls) living in rural areas, with ages ranging from 3 to 15 years old and various clinical histories, were included in this study. Eight patients had only liver hydatid cyst, while 1 patient had liver and lung hydatid cyst and the other liver, lung, and spleen, together. There were 2 ruptured liver cysts. After surgery, during follow-up, no increase was observed in hemagglutination levels, there were no mortalities, and there was no evidence of recurrence at 2 years post operation in all patients. Molecular analysis was performed on hydatid cyst samples obtained from the 10 pediatric cases. According to mt-12S rRNA PCR results, all cases were found to be G1/G3 cluster of E. granulosus sensu stricto.

Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey

“…As a result, all the isolates collected in this study were confirmed by BLAST algorithm as E. granulosus s.l. From Turkey, there are several reports on molecular characterization of E. granulosus s.l isolates from various intermediate hosts (Schneider et al 2008;Utuk et al 2008;Snabel et al 2009;Ergin et al 2010;Simsek et al 2011;Utuk and Simsek 2013;Bakal et al 2015;Eroglu et al 2016;Orsten et al 2018;Kurt et al 2020;Örsten et al 2020). These studies showed that E. granulosus s.s. (genotypes G1-G3) is the predominant species obtained from hydatid materials in Turkey.…”

Investigation of the relationship between CE cyst characteristics and genetic diversity of Echinococcus granulosus sensu lato in humans from Turkey

“…There is a lot of research conducted on molecular identification of E. granulosus s.l. isolates from sheep, cattle, goats and humans from different regions of Turkey (Schneider et al ., 2010; Utuk et al ., 2008; Šnàbel et al ., 2009; Ergin et al ., 2010; Simsek et al ., 2011; Eryildiz and Şakru, 2012; Bakal et al ., 2015; Eroğlu et al ., 2016; Orsten et al ., 2018). According to these studies, E. granulosus s.s. (G1–G3 genotypes) was the most common species causing CE in Turkey.…”

“…Several papers indicated that G1 and G3 genotypes ( E. granulosus s.s. ) are predominant in the human host in Turkey (Utuk et al ., 2008; Snabel et al ., 2009; Ergin et al ., 2010; Simsek et al ., 2011; Eryıldız and Şakru, 2012; Bakal et al ., 2015; Eroğlu et al ., 2016; Erdoğan et al ., 2017; Oral Babaoğlu et al ., 2018; Orsten et al ., 2018). Echinococcus canadensis (G7 genotype), also known as pig strain, was reported from Turkey for the first time by Snabel et al .…”

A study to assess the awareness of risk factors of cystic echinococcosis in Turkey

“…In a study performed by Bakal et al contact with dogs and poor hygiene conditions were found to be important risk factors for CE. 38 Non effective control programs for E.granulosus in dogs prevent eradication of this parasite. 39 In our study, 77.5% of all dog owners administered did not administer antiparasitic drugs to their dogs; in Sardinia, Italy, the corresponding rate was 90%.…”