Surgical and histopathological effects of topical ankaferd hemostat on major arterial vessel injury in relation to the enhanced intra-arterial blood pressure

Abstract

Objective: The aim of this study was to assess the surgical and histopathological hemostatic effects of topical Ankaferd blood stopper (ABS) on major arterial vessel injury related to elevated intra-arterial blood pressure in an experimental rabbit model. Materials and Methods: The study included 14 New Zealand rabbits. ABS was used to treat femoral artery puncture on 1 side in each animal and the other untreated side served as the control. Likewise, for abdominal aortic puncture, only 50% of the aortic injuries received topical liquid ABS and the others did not (control). The experiment was performed under conditions of normal arterial blood pressure and was repeated with a 50% increase in blood pressure. Histopathological analysis was performed in all of the studied animals. Results: Mean bleeding time in the control femoral arteries was 105.0±18.3 s, versus 51.4±9.8 s (p<0.05) in those treated with ABS. Mean blood loss from the punctured control femoral arteries was 5.0±1.5 mg and 1.6±0.4 mg from those treated with ABS (p<0.05). Histopathological examination of the damaged arterial structures showed that ABS induced red blood cell aggregates. Conclusion: ABS administered to experimental major arterial vessel injury reduced both bleeding time and blood loss under conditions of normal and elevated intra-arterial blood pressure. ABS-induced erythroid aggregation was prominent at the vascular tissue level. These findings will inform the design of future experimental and clinical studies on the anti-bleeding and vascular repairing effects of the novel hemostatic agent ABS. (Turk J Hematol 2011; 28: 206-12)

Experimental Investigation of the Effects of a Blood Stopper Agent (Ankaferd Blood Stopper) on Bone Surfaces

“…The hemostatic properties of ABS have been demonstrated by studies carried out in dentistry and other fields [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. Baykul et al reported at the end of a study performed on 4 patients with von Willebrand disease, chronic liver disease, and mitral valve replacement that ABS was an effective agent in stopping localized bleeding during tooth extraction with diathesis patients [ 15 ].…”

“…In addition, the combination of these plants in ABS appears to provide a unique composition for tissue oxygenation and the physiological hemostatic process without disturbing the levels of any individual clotting factor [ 7 ]. Various experimental and clinical studies have demonstrated the effect of ABS on bleeding control [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ].…”

Effect of Ankaferd Blood Stopper on muscle healing

“…The effectiveness of ABS has been proven as an efficient hemostatic agent and is still used in emergency situations. Topical application of ABS in an experimental major arterial vessel injury model reduced bleeding time and blood loss [8]. Also ABS seems to be an effective hemostatic agent for patients undergoing total thyroidectomy [9] and adenoidectomy [10].…”

“…ABS is also used as a hemostatic agent in acute intractable bleeding and surgery [7][8][9][10]. Hematoma is known to take an active role in wound healing [8], and preventing hematoma may have a negative effect on wound healing. Reduced hematoma may also decrease fibrosis.…”

Efficacy of Ankaferd Blood Stopper in Bleeding Control in Experimental Partial Splenectomy Model

“…There are experimental studies in literature, showing the effects of ABS on arterial wall and tissue. In the experimental arterial model developed by Ulus et al 20 they showed that topical ABS treatment decreased bleeding time and bleeding amount at normal and high arterial pressures. In the experimental epistaxis model developed by Iynen et al 21 they determined that topical ABS ensures more effective homeostasis than saline irrigation and shortens the bleeding time.…”