Surfactin production in the bioreactor: Emphasis on magnetic nanoparticles application

Abstract

Surfactin is one of the main lipopeptide biosurfactants produced by different species of Bacillus subtilis. This study aims to analyze the effect of starch‐coated Fe0 and Fe3+ nanoparticles on the biomass and biosurfactant production of Bacillus subtilis. Out of 70 soil samples, 20 Bacillus were isolated and genome sequenced by biochemical methods and 16S rRNA gene. Quantitative and qualitative screening methods were used to isolate and detect biosurfactant production. For the aim of this study, 61 and 63 (Bacillus subtilis subsp. Inaquosorum) were selected. Then, hemolytic activity, biomass amount, surfactant production, and reduction of surface tension in Minimal Salt Medium containing Fe0 and Fe3+ nanoparticles were examined after 48, 72, and 96 h of culture. Strain 61 was the best bacterium and Fe3+ was the best nanoparticle. The results were compared with the results of non‐nanoparticle bioreactor. The results showed the amount of biomass, surfactin, and surface tension decrease, 72 h after growth in 61 strain containing Fe3+ reached the highest values. Surfactin from strain 61 culture in the Fe3+nanoparticle bioreactor after 72 h of growth showed higher production than the same strain culture after 72 h without Fe3+, if continuing the research, this strain can be commercialized in the future.

Evaluation of SDS‐coated iron nanostructure on the gene expression of bio surfactant‐producing genes by Pseudomonas aeruginosa

“…In this study, starch‐coated iron nanoparticles did not show any bacterial toxicity at 1mg/ml and increased the growth rate and production of bio surfactants to 20.62% [19]. Modabber et al., was investigated the effect of starch coated Fe 0 and Fe 3+ nanoparticles on the production of surfactin from Bacillus subtilis [20]. The expression of most Pseudomonas aeruginosa genes is controlled by the Quorum sensing (QS) gene system.…”

Evaluation of production of lipopeptide biosurfactants and surfactin micelles by native Bacillus of Iran, for a broader application range

“…Modabber et al used starch‐coated Fe 3+ and Fe 0 nanoparticles to increase surfactin production. The results showed that the optimal concentration Fe 3+ nanoparticles which has positive effects on the production of bacterial biosurfactants was of 1 mg/L (Modabber et al, 2020). In the study by Young et al, the effect of Fe nanoparticles on increasing surfactant production in Bacillus amyloliquefaciens was investigated.…”