Surfactant enhances biodegradation of hydrocarbons: Microcosm and field study

Walter, M. V.; Nelson, Eryn; Firmstone, G. P.; Martin, David G.; Clayton, Matt; Simpson, Stuart L.; Spaulding, Sarah A.

doi:10.1080/15320389709383546

Cited by 21 publications

(6 citation statements)

References 9 publications

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“…There was no significant variation in the bacterial population in all the amendments at 1% probability level. Walter et al (1997) reported similar observations with oil contaminated soil under field condition while testing anionic surfactant guanidinium cocoate amended with mixed consortium and vermiculite.…”

Section: Resultssupporting

confidence: 65%

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Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipid biosurfactant

Rahman

Banat

Thahira

et al. 2002

Bioresource Technology

205

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Section: Resultssupporting

confidence: 65%

“…For petroleum hydrocarbons, these biological waste products are primarily CO 2 , water and methane (Walter et al, 1997). As no single microbial species is capable of degrading all components of crude oil, complete oil degradation requires simultaneous action of different microbial populations.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipid biosurfactant

Rahman

Banat

Thahira

et al. 2002

Bioresource Technology

205

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“…As a major component of petroleum, alkanes have become an important source of pollution in the environment because of their extensive use ( Walter et al . 1997 ).…”

Section: Introductionmentioning

confidence: 99%

Role of rhamnolipid biosurfactants in the uptake and mineralization of hexadecane in Pseudomonas aeruginosa

2000

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A study was undertaken to investigate the mechanisms for biosurfactant-enhanced hexadecane uptake into Pseudomonas aeruginosa. Two strains of Ps. aeruginosa were studied, one producing rhamnolipids (PG201) and the other rhamnolipid de®cient (UO299). Rhamnolipids produced by PG201 acted to increase the solubility of nhexadecane in the culture medium (from 1Á84 to 22Á76 mg l À1 ). Rates of 14 C-n-hexadecane uptake and mineralization were higher in PG201 than in UO299. However, the degree of difference was lower than expected. Additional studies were carried out on the cell surface properties of the two strains. During growth on n-hexadecane, the cell surface hydrophobicity of both PG201 (50Á5%) and UO299 (33Á7%) increased compared with that observed in water-soluble growth substrates (7±8%). Studies were also carried out to ascertain any energy requirements for the transport of n-hexadecane into Ps. aeruginosa cells. The addition of CCCP (an inhibitor of cytochrome oxidase which thereby blocks oxidative phosphorylation) at a range of concentrations caused a marked decrease in nhexadecane uptake, indicating that n-hexadecane uptake in Ps. aeruginosa is an energydependent process. These studies support the hypothesis of alkane transport into microbial cells by direct contact with larger alkane droplets and by pseudosolubilization. Also, it appears that both mechanisms occur simultaneously.

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“…This is due to its low cost and ability to halt the accumulation of contaminants (El-Nawawy et al, 1987;April et al, 2000;Jain et al, 2011). Bioremediation refers to the decomposing of organic pollutants by soil microorganism with subsequent conversion of the decomposed materials to harmless end products such as carbon dioxide, water and methane (Walter et al, 1997). Bioremediation is not only useful in the recovery of site contaminated with oil but also applicable to recovery of hazardous wastes contaminated sites or media (Chitra and Lakshmanaperumalsamy, 2010;Rezaee et al, 2010;Thavasi et al, 2006;Saber et al, 2010;Akinyele et al, 2011;Amini et al, 2011;Ogwal et al, 2011).…”

Section: Microbial J 1 (4): 139-148 2011mentioning

confidence: 99%

Preliminary Study on Biodegradation of Nigerian Natural Bitumen

Olabemiwo¹,

Adediran²,

Adekola³

et al. 2011

Microbiology J.

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One of the major problems facing the existence of man in this millennium is pollution. Thus, it is no surprise that much attention is given to pollution control and remediation of polluted environment. While remediation can be achieved by physicochemical and biological methods, application of the latter tends to be gaining upper hand due to some of its inherent advantages. This study investigated the biodegradability of bitumen from Agbabu, Southwestern Nigeria by hydrocarbon utilizing strains of Pseudomonas putrefaciens, Pseudomonas nigrificans, Bacillus licheniformis, Pseudomonas tragi and Achromobacter aerogenes. The organisms were used to inoculate Mineral Salts Medium (MSM) supplemented with 0.2 g L-1 solution of Agbabu bitumen in dichloromethane. Cultures were inoculated at different temperature and pH (27°C/pH 3.5; 27°C/pH 5.6; 30°C/pH 7.0 and 40°C/pH 7.0) for two weeks. The quantity of bitumen degraded by each organism was determined gravimetrically at the end of first and second week. The rate of degradation was calculated for each organism and residual bitumen analyzed by infraredSpectroscopy. In the first week of incubation, Achromobacter aerogenes exhibited the highest rate of degradation (1.750±0.027 mg h-1 ) at 30°C and pH 7 while Bacillus licheniformis showed the least degradation rate (0.300±0.018 mg h-1 ) at 40°C and pH 7. Structural indices such as aromaticity, sulphonation, aliphaticity and oxidation calculated from the infrared spectra of recovered bitumen from the inoculated samples were different from that of the control. This thus, confirms the degradation capability of the bacteria used in this work on the Agbabu bitumen and hence their potentials for use in bioremediation of bitumen-polluted environments.

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Surfactant enhances biodegradation of hydrocarbons: Microcosm and field study

Cited by 21 publications

References 9 publications

Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipid biosurfactant

Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipid biosurfactant

Role of rhamnolipid biosurfactants in the uptake and mineralization of hexadecane in Pseudomonas aeruginosa

Preliminary Study on Biodegradation of Nigerian Natural Bitumen

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