[1] The stable isotopic compositions of bulk, clay-bound, organic, and compound-specific nitrogen were determined for mid to late Holocene Black Sea sediments from a set of box and gravity cores. The data demonstrate that cyanobacterial N 2 fixation provided $55% of phytoplankton-derived N preserved in the top 1-2 cm of the sediments. Prior to widespread agricultural and industrial development in the catchment, N 2 fixation was more prominent, providing 70-80% of phytoplankton N. Organic and clay-bound nitrogen fractions record different down-core d 15 N trends that reflect phytoplankton and detrital sources, respectively, and in samples with low organic matter content, the clay-bound fraction comprises up to 38% of bulk nitrogen. Compared with bulk samples, pyropheophytin a (Pphe a), which is a chlorophyll a (Chl a) degradation product, provides a more accurate record of changing phytoplankton d