“…Previously, Min proteins were reconstituted in vitro (Loose et al, 2008; Ivanov and Mizuuchi, 2010; Loose et al, 2011a; Schweizer et al, 2012; Martos et al, 2013; Vecchiarelli et al, 2014; Zieske et al, 2014; Martos et al, 2015; Vecchiarelli et al, 2016), and Min oscillations were observed in fabricated microchambers (Zieske and Schwille, 2013, 2014). In the latter case, the microchambers had a half-open configuration (i.e., with a top surface not coated with SLB) with a limited width (∼10 µm), a height of 10 µm, and varying lengths leading to a range of aspect ratios (1.2–24).…”