“…Therefore, factors such as temperature (Touhami et al, 2006;Caspi and Dekker, 2016), membrane composition (Mileykovskaya and Dowhan, 2000;Koppelman et al, 2001;Mileykovskaya et al, 2003;Szeto et al, 2003;Hsieh et al, 2010;Weibel, 2011, 2012;Shih et al, 2011;Vecchiarelli et al, 2014;Zieske and Schwille, 2014), diffusion in the cytosol (Meacci et al, 2006;Schweizer et al, 2012;Martos et al, 2015;Caspi and Dekker, 2016) and on the membrane (Meacci et al, 2006;Martos et al, 2013), the concentration ratio of MinD to MinE (Raskin and de Boer, 1999b;Shih et al, 2002;Loose et al, 2008;Vecchiarelli et al, 2016;Kretschmer et al, 2017;Miyagi et al, 2018) and interaction of MinE with the membrane (Hsieh et al, 2010;Loose et al, 2011a;Park et al, 2011;Shih et al, 2011;Zieske and Schwille, 2014;Vecchiarelli et al, 2016;Kretschmer et al, 2017) can also modulate the Min behavior and cause a difference in the specific length scale in in vivo and reconstituted systems (see Table S2). Reconstitution experiments helped, for example, to characterize the role of the membranetargeting sequence of MinE; Kretschmer et al showed that membrane binding of MinE is not a requirement for Min oscillations, but that it modulates the length scale of the pattern (Kretschmer et al, 2017). Experiments with higher diffusion constants, representing the absence of molecular crowding in the cytosol and on the membrane, showed that these factors account for the increased length scale in vitro (Martos et al, 2013…”