Surface Properties in Relation to Atelectasis and Hyaline Membrane Disease

Avery, Mary Ellen; Mead, J.

doi:10.1001/archpedi.1959.02070010519001

Cited by 794 publications

(585 citation statements)

References 8 publications

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“…Deficiency in lung surfactant leads to conditions such as neonatal respiratory distress syndrome (RDS), which is a crucial problem in premature newborns (6), as well as acute respiratory distress syndrome (ARDS) (7), a sudden lung failure associated with acute injury or illness. The clinical introduction of surfactant replacement therapy in the early 1990s dramatically improved pulmonary morbidity and mortality in premature infants with RDS (8) and may also be effective in ARDS (9).…”

mentioning

confidence: 99%

NMR Structures of the C-Terminal Segment of Surfactant Protein B in Detergent Micelles and Hexafluoro-2-propanol

et al. 2004

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Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have used NMR to determine the structure of a C-terminal fragment of human SP-B that includes residues 63-78. Structure determination was performed both in the fluorinated alcohol hexafluoro-2-propanol (HFIP) and in sodium dodecyl sulfate (SDS) micelles. In both solvents, residues 68-78 take on an amphipathic helical structure, in agreement with predictions made by comparison to homologous saposin family proteins. In HFIP, the five N-terminal residues of the peptide are largely unstructured, while in SDS micelles, these residues take on a well-defined compact conformation. Differences in helical residue side chain positioning between the two solvents were also found, with better agreement between the structures for the hydrophobic face than the hydrophilic face. A paramagnetic probe was used to investigate the position of the peptide within the SDS micelles and indicated that the peptide is located at the water interface with the hydrophobic face of the helix oriented inward, the hydrophilic face of the helix oriented outward, and the N-terminal residues even farther from the micelle center than those on the hydrophilic face of the R-helix. Interactions of basic residues of SP-B with anionic lipid headgroups are known to have an impact on function, and these studies demonstrate structural ramifications of such interactions via the differences observed between the peptide structures determined in HFIP and SDS.

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confidence: 99%

NMR Structures of the C-Terminal Segment of Surfactant Protein B in Detergent Micelles and Hexafluoro-2-propanol

et al. 2004

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“…The observation that lungs from premature infants who died of the respiratory distress syndrome contained less surface active material than did the lungs of infants dying from other causes (2) led to extensive developmental studies of lung phospholipid metabolism using animal models (5,11,12,32,36). Such studies have resulted in clinical tests to predict lung maturity in utero (9, lo), in methods to "induce" lung maturation in utero with corticosteroids (27,33), and in improved therapy for infants with the respiratory distress syndrome (13,34).…”

Section: Speculationmentioning

confidence: 99%

Surfactant Phospholipid Metabolism in 3-Day and 3-Day Postmature Rabbits In Vivo

Jobe

1980

Pediatr Res

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Summary remain controversial (3 1). Radiolabeled phospholipid precursorsThree-day-old normal and M a y postmature rabbits (prepared by human chorionic gonadotropin treatment of the does) were given injections of the radiolabeled phospholipid precursors pnlmitic acid, choline, and glycerol. The specific activities as c p d pool phospholipid phosphate were measured for phosphatidykholine, disaturated phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, and phosphatidylethanolPmine from lung and alveolar wash fraction lipid extracts of May-old rabbits. Specific activities similarlv were measured for ~hos~hatidvlcholine and disaturated ph~s~hatid~lcholine from p&tmabre ribbits. Curves describing the appearance of labeled phospholipids into the alv* lar wash fractiondemonstrated that 6 both 3-day and postmature rabbits, phospholipid secretion was delayed for 3 to 4 hr. Thereafter, there was a rapid accumulation of labeled phospholipids until 12 to 16 hr after labeled precursor injection. The curves were similar for all measured phospholipids and all labeled precursors studied. The phospholipid compositions of lung and alveolar wash fraction lipid extracts were determined by analysis of the various phospholipids separated by two-dimensional thin-layer chromatography. Forty percent of lung samples and 35% of alveolar wash samples from postmature rabbits contained no detectable phosphatidylglycerol, whereas all 22 lung and surfactant samples from 3-day-old rabbits contained this phospholipid. Speculation

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“…It is responsible for decreasing the surface tension of the air-liquid interface of the alveoli, thus preventing lung collapse at the end of expiration (1).…”

Section: Introductionmentioning

confidence: 99%

Surfactant protein B gene polymorphism in preterm babies with respiratory distress syndrome

Lyra

Diniz

Abe‐Sandes³

et al. 2011

Braz J Med Biol Res

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Surface Properties in Relation to Atelectasis and Hyaline Membrane Disease

Cited by 794 publications

References 8 publications

NMR Structures of the C-Terminal Segment of Surfactant Protein B in Detergent Micelles and Hexafluoro-2-propanol

NMR Structures of the C-Terminal Segment of Surfactant Protein B in Detergent Micelles and Hexafluoro-2-propanol

Surfactant Phospholipid Metabolism in 3-Day and 3-Day Postmature Rabbits In Vivo

Surfactant protein B gene polymorphism in preterm babies with respiratory distress syndrome

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