Surfactant protein B gene polymorphism in preterm babies with respiratory distress syndrome

Lyra, Priscila Pinheiro Ribeiro; Diniz, Edna Maria de Albuquerque; Abe‐Sandes, Kiyoko; Angelo, Ana Luiza Dias; Machado, Taisa Manuela Bonfim; Cardeal, Mauricio

doi:10.1590/s0100-879x2010007500147

Cited by 18 publications

(15 citation statements)

References 32 publications

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“…Studies have demonstrated that RDS is a multifactorial and multi- •P<0.001 vs control group Unauthenticated Download Date | 5/11/18 3:12 PM gene disease in which there is interaction among genetic factors, environmental factors, preterm, gender, race and maternal factors (9). Some surfactantassociated proteins (especially SP-A and SP-B) are susceptible to mutation, and detrimental or protective in the RDS (1). The relationship between deficiency in the surfactant and neonatal RDS has been confirmed (10)(11)(12).…”

Section: Discussionmentioning

confidence: 99%

“…Pulmonary surfactant-associated proteins (SP) include SP-A, SP-B, SP-C and SP-D which play pivotal roles in the function and metabolism of pulmonary surfactant. SP-B is one of the important SPs and can reduce or alter the surface tension via changing the surface area, which prevents the alveolar collapse (1)(2)(3)(4)(5). Respiratory distress syndrome (RDS) is a multifactorial and multi-gene disease, and a genetic factor has been found to be involved in the pathogenesis of RDS.…”

Section: Introductionmentioning

confidence: 99%

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Expression of Pulmonary Surfactant-Associated Protein B in Neonatal Respiratory Distress Syndrome

Yin¹,

Wang²,

Xie³

et al. 2013

Journal of Medical Biochemistry

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SummaryBackground: The aim of this study was to investigate the role of pulmonary surfactant-associated protein B (SP-B) expression in the pathogenesis of neonatal respiratory distress syndrome (RDS) via detecting the protein and mRNA expression of SP-B. Methods: A total of 60 unrelated neonates who died of RDS were chosen as the RDS group and then subgrouped into ≤32 weeks group, 32∼37 weeks group and ≥37 weeks group (n=20). Sixty neonates who died of other diseases were enrolled as controls and subdivided into 3 matched groups based on the gestational age. Western blot assay and RT-PCR were employed. Results: In the RDS group, SP-B protein expression was reduced or deficient in 8 neonates of which 6 had no SP-B protein expression. In the control group, only 1 had reduced SP-B protein expression. The reduced or deficient SP-B protein expression in 9 neonates of both groups was noted in the ≥37 weeks group. In the RDS group, the SP-B mRNA expression was significantly lower than that in the control group. In the ≤37 weeks group, SP-B mRNA expression was comparable between the RDS group and control group. In the 32∼37 weeks group, the SP-B mRNA expression in the RDS group was significantly reduced when compared with the control group. In the ≥37 weeks group, the SP-B mRNA expression in the RDS group was dramatically lower than that in the control group. Conclusions: Alteration of SP-B expression is present at transcriptional and translational levels. Reduction of SP-B mRNA and protein expression is involved in the pathogenesis of RDS.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of Pulmonary Surfactant-Associated Protein B in Neonatal Respiratory Distress Syndrome

Yin¹,

Wang²,

Xie³

et al. 2013

Journal of Medical Biochemistry

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“…SP-B and SP-C are hydrophobic, and especially SP-B has a pivotal role in the absorption and distribution of surfactant (8). SP-B is required for the synthesis of lamellar bodies, the aggregation of phospholipids into the OLB, and for the reduction of surface tension at the air-liquid interphase (1,57,58). SP-A and SP-B (and, to a lesser extent, SP-C) promote the formation of the surfactant monolayer, which is the functionally active form of surfactant and possesses the surface-active properties (1).…”

mentioning

confidence: 99%

“…SP-A and SP-B (and, to a lesser extent, SP-C) promote the formation of the surfactant monolayer, which is the functionally active form of surfactant and possesses the surface-active properties (1). Whereas mutations in the SP-C-gene have been associated with neonatal RDS and interstitial lung disease, the absence of SP-B has led to respiratory failure and death shortly after birth (10,57,58). Concerning SP-D, animal studies show that SP-D does not participate in the normal development of the lung but may have a role in the development of emphysema and fibrosis later in life (10).…”

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confidence: 99%

The impact of vitamin D on fetal and neonatal lung maturation. A systematic review

Lykkedegn

Sørensen

Beck-Nielsen

et al. 2015

American Journal of Physiology-Lung Cellular and Molecular Phys

106

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-Respiratory distress syndrome (RDS) and bronchopulmonary dysplasia (BPD) are major complications to preterm birth. Hypovitaminosis D is prevalent in pregnancy. We systematically reviewed the evidence of the impact of vitamin D on lung development, surfactant synthesis, RDS, and BPD searching PubMed, Embase, and Cochrane databases with the terms vitamin D AND (surfactant OR lung maturation OR lung development OR respiratory distress syndrome OR fetal lung OR prematurity OR bronchopulmonary dysplasia). Three human studies, ten animal studies, two laboratory studies, and one combined animal and laboratory study were included. Human evidence was sparse, allowing no conclusions. BPD was not associated with vitamin D receptor polymorphism in a fully adjusted analysis. Animal and laboratory studies showed substantial positive effects of vitamin D on the alveolar type II cell, fibroblast proliferation, surfactant synthesis, and alveolarization. These data support the hypothesis of hypovitaminosis D as a frequent, modifiable risk factor of RDS and BPD, which should be tested in randomized controlled trials on pregnant women, those with threatening preterm delivery, or in the preterm neonates. Future experimental and human studies should aim to identify optimal time windows, vitamin D doses, and cut-off levels for 25-hydroxyvitamin D in interventions against RDS, BPD, and later adverse respiratory outcomes. vitamin D; fetus; lung; surfactant; preterm; neonate THE IMPORTANCE OF VITAMIN D in nonskeletal fetal and childhood health has become increasingly recognized in the last decades (19,65). The impact of vitamin D on early lung development and maturation and lung diseases of early life is an emerging field of research. Hypovitaminosis D during pregnancy is associated with reduced placental development and weight, leading to a presumed risk of preterm birth (24,25), which per se may lead to respiratory distress syndrome (RDS) and bronchopulmonary dysplasia (BPD). Moreover, vitamin D is suggested to play a role in the embryogenesis and in cellular growth and differentiation, including lung development and regulation of lung maturation in the fetus (23,29,38,70,71,108), whereby hypovitaminosis D could be hypothesized to aggravate premature neonatal lung diseases.Vitamin D is involved in the development of the innate and adaptive immune system, and, because of its unique capacity to bind to the vitamin D receptor (VDR) and thereby to serve as a transcriptional factor, vitamin D can regulate gene expression and exert widespread immunomodulatory effects including proliferation, differentiation, and complex regulation (19, 56, 58 -61, 64, 65, 68, 69, 105). Evidence suggests that vitamin D may stimulate regulatory T cell development and T cell function to suppress inappropriate Th1 and Th2 responses to environmental exposure (i.e., allergens, infection load), leading to a more balanced immune response inhibiting autoimmune diseases (with Th1 dominance) and allergic diseases (Th2 dominance) (55). Vitamin D could therefore be inv...

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“…Results have shown that SP-B deficiency is correlated with certain pulmonary diseases including neonatal RDS and chronic obstructive pulmonary (17,18). Recent ongoing studies have investigated this correlation yielding primary results (19).…”

Section: Discussionmentioning

confidence: 99%

Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome

Yin

Fan

et al. 2012

Experimental and Therapeutic Medicine

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The aim of this study was to investigate whether surfactant-associated protein B (SP-B) mRNA deficiency is involved in the pathogenesis of neonatal respiratory distress syndrome (RDS). A total of 60 unrelated neonates who died of RDS were recruited as the RDS group and subgrouped into a ≤32-, a 32–36+6- and a ≥37-week group (n=20 per group) on the basis of gestational age. In addition, 60 neonates who succumbed to other diseases were enrolled as controls. The lung tissues were collected within 30 min after death. In situ hybridization was conducted to detect SP-B mRNA expression in the lung. The frequency of SP-B mRNA deficiency was also calculated. Among the RDS groups, the SP-B mRNA levels were significantly higher compared to those in the control group (t=7.812, P<0.001), but were comparable among RDS patients with different gestational ages (F=2.348, P>0.105). Among the control groups, the SP-B mRNA levels increased with the increase in gestational age (F=50.124, P<0.001). In the ≤32-week group, the number of cells positive for SP-B mRNA in RDS patients was markedly reduced as compared to that of the controls (t=3.185, P<0.01). In the 32–36+6-week group, the number of cells positive for SP-B mRNA in RDS patients was significantly smaller compared to that of the controls (t=9.342, P<0.001). In the ≥37-week group, the number of cells positive for SP-B mRNA in RDS patients was markedly smaller compared to that in the controls (t=4.238, P<0.001). Among RDS neonates, SP-B mRNA deficiency was noted in 35 patients with a frequency of 58.3%. In the control group, SP-B mRNA deficiency was noted in 8 patients with a frequency of 13.3%, which was markedly lower compared to that in the RDS group (χ2=26.421, P<0.001). The results of the present study therefore suggest that SP-B mRNA deficiency is involved in the pathogenesis of RDS.

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Surfactant protein B gene polymorphism in preterm babies with respiratory distress syndrome

Cited by 18 publications

References 32 publications

Expression of Pulmonary Surfactant-Associated Protein B in Neonatal Respiratory Distress Syndrome

Expression of Pulmonary Surfactant-Associated Protein B in Neonatal Respiratory Distress Syndrome

The impact of vitamin D on fetal and neonatal lung maturation. A systematic review

Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome

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