Surface potential and charges impact on cell responses on biomaterials interfaces for medical applications

Metwally, Sara; Stachewicz, Urszula

doi:10.1016/j.msec.2019.109883

Cited by 178 publications

(113 citation statements)

References 238 publications

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“…Cell-material interactions are highly complex and require more systematic investigations regarding surface charge. It is certain that electrostatic forces are crucial for cellular attachment via focal adhesion to material surfaces ( Metwally and Stachewicz, 2019 ) influencing the further fate of cells. Earlier studies have mostly dealt with a limited number of surface charges [e.g., only negative surfaces ( Altankov et al, 2003 ), one positive compared with several negative surfaces ( Chang et al, 2014 ), or only one negative, neutral and positive surface charge ( Iwai et al, 2013 )] disallowing detailed statements.…”

Section: Discussionmentioning

confidence: 99%

“…As human osteoblasts are negatively charged (Rebl et al, 2016), positive surface charges significantly influence cell adhesion (Rebl et al, 2010;Dhowre et al, 2015;Mörke et al, 2017), spreading and proliferation (Staehlke et al, 2019), particularly in the early stages of cell responses. The most detailed study on the effect of surface charges to date is that of Metwally and Stachewicz (2019), indicating a great importance in the development of functionally implantable biomaterials. They reviewed that surface charges determine protein adsorption and thus the subsequent cell adhesion process.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

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Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between −90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (−90 to −3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials' zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…There is no general trend that describes the relationship between the surface roughness and the cell response; it always depends also on other physicochemical properties of the biomaterials and the specific cells and their phenotype [ 21 ]. The surface charge of the material strongly affects the cell behavior [ 22 ] and it can be regulated through the chemical functional groups in the polymer chains. The positively charged surface has been proved to provide significantly better cell adhesion to the material surface or the cell-cell interaction than the negatively charged surface [ 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

et al. 2021

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The development of new biocompatible polymer substrates is still of interest to many research teams. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with a technique of improved phase separation. Plasma exposure served for substrate activation and modification of surface properties, such as roughness, chemistry, and wettability. The treated FEP substrate was applied for the growth of a honeycomb-like pattern from polystyrene solution. The properties of the pattern strongly depended on the primary plasma exposure of the FEP substrate. The physico-chemical properties such as changes of the surface chemistry, wettability, and morphology of the prepared pattern were determined. The cell response of primary fibroblasts and osteoblasts was studied on a honeycomb pattern. The prepared honeycomb-like pattern from polystyrene showed an increase in cell viability and a positive effect on cell adhesion and proliferation for both primary fibroblasts and osteoblasts.

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“…PS is mostly used for in vitro studies [4,5,32], but without any surface modification, for example, sliver negative ion implementation [33], protein absorption [34], or plasma treatment [35,36], does not enhance cell development. PS fibers have been already combined with PA6 fibers [37] in the fog collector's meshes, comparing fiber diameter, roughness, contact angle, and the showing mechanical properties of PS, PA6, and PS-PA6 mats of maximum stress 0.03, 1.24 and 0.07 MPa, respectively [38].…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Composite Meshes of Electrospun PS Microfibers with PA6 Nanofibers for Regenerative Medicine

et al. 2020

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One of the most frequently applied polymers in regenerative medicine is polystyrene (PS), which is commonly used as a flat surface and requires surface modifications for cell culture study. Here, hierarchical composite meshes were fabricated via electrospinning PS with nylon 6 (PA6) to obtain enhanced cell proliferation, development, and integration with nondegradable polymer fibers. The biomimetic approach of designed meshes was verified with a scanning electron microscope (SEM) and MTS assay up to 7 days of cell culture. In particular, adding PA6 nanofibers changes the fibroblast attachment to meshes and their development, which can be observed by cell flattening, filopodia formation, and spreading. The proposed single-step manufacturing of meshes controlled the surface properties and roughness of produced composites, allowing governing cell behavior. Within this study, we show the alternative engineering of nondegradable meshes without post-treatment steps, which can be used in various applications in regenerative medicine. supervision, U.S.; project administration, U.S.; funding acquisition, U.S. All authors have read and agreed to the published version of the manuscript.

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Surface potential and charges impact on cell responses on biomaterials interfaces for medical applications

Cited by 178 publications

References 238 publications

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

Hierarchical Composite Meshes of Electrospun PS Microfibers with PA6 Nanofibers for Regenerative Medicine

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