Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

Fajstavrová, Klára; Rimpelová, Silvie; Fajstavr, Dominik; Švorčı́k, Václav; Slepička, Petr

doi:10.3390/ma14040889

Cited by 7 publications

(8 citation statements)

References 54 publications

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“…Milled acrylic was slightly pro-osteoblastic at all time points. In another study examining the cytotoxicity of different surface treatments of composite, there was a trend towards a pro-osteoblastic phenotype [ 59 ], consistent with our results. We separately evaluated the contact and proximity effects of each material for fibroblasts and osteoblasts and provided robust, fundamental data, which warrants further studies such as in vitro mechanistic studies and animal studies.…”

Section: Discussionsupporting

confidence: 91%

Cell Type-Specific Effects of Implant Provisional Restoration Materials on the Growth and Function of Human Fibroblasts and Osteoblasts

et al. 2022

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Implant provisional restorations should ideally be nontoxic to the contacting and adjacent tissues, create anatomical and biophysiological stability, and establish a soft tissue seal through interactions between prosthesis, soft tissue, and alveolar bone. However, there is a lack of robust, systematic, and fundamental data to inform clinical decision making. Here we systematically explored the biocompatibility of fibroblasts and osteoblasts in direct contact with, or close proximity to, provisional restoration materials. Human gingival fibroblasts and osteoblasts were cultured on the “contact” effect and around the “proximity” effect with various provisional materials: bis-acrylic, composite, self-curing acrylic, and milled acrylic, with titanium alloy as a bioinert control. The number of fibroblasts and osteoblasts surviving and attaching to and around the materials varied considerably depending on the material, with milled acrylic the most biocompatible and similar to titanium alloy, followed by self-curing acrylic and little to no attachment on or around bis-acrylic and composite materials. Milled and self-curing acrylics similarly favored subsequent cellular proliferation and physiological functions such as collagen production in fibroblasts and alkaline phosphatase activity in osteoblasts. Neither fibroblasts nor osteoblasts showed a functional phenotype when cultured with bis-acrylic or composite. By calculating a biocompatibility index for each material, we established that fibroblasts were more resistant to the cytotoxicity induced by most materials in direct contact, however, the osteoblasts were more resistant when the materials were in close proximity. In conclusion, there was a wide variation in the cytotoxicity of implant provisional restoration materials ranging from lethal and tolerant to near inert, and this cytotoxicity may be received differently between the different cell types and depending on their physical interrelationships.

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Section: Discussionsupporting

confidence: 91%

Cell Type-Specific Effects of Implant Provisional Restoration Materials on the Growth and Function of Human Fibroblasts and Osteoblasts

et al. 2022

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“…After additional cultivation, 96 h post-seeding, cell proliferation was obvious on each evaluated sample. The lowest number of cells was detected on the unmodified FEP sample, which is consistent with our previous results, e.g., [58][59][60]. The cell number was increased on plasma-treated FEP 96 h after seeding when compared to pristine FEP.…”

Section: Cytocompatibilitysupporting

confidence: 92%

Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer

et al. 2021

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In this study, we present a simple approach for developing a biocompatible polymer scaffold with a honeycomb-like micropattern. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with an improved phase separation technique. The plasma exposure served for modification of the polymer surface properties, such as roughness, surface chemistry, and wettability. The treated FEP substrate was applied for the growth of a honeycomb-like pattern from a solution of polymethyl methacrylate (PMMA). The properties of the pattern were strongly dependent on the conditions of plasma exposure of the FEP substrate. The physico-chemical properties of the prepared pattern, such as changes in wettability, aging, morphology, and surface chemistry, were determined. Further, we have examined the cellular response of human osteoblasts (U-2 OS) on the modified substrates. The micropattern prepared with a selected combination of surface activation and amount of PMMA for honeycomb construction showed a positive effect on U-2 OS cell adhesion and proliferation. Samples with higher PMMA content (3 and 4 g) formed more periodic hexagonal structures on the surface compared to its lower amount (1 and 2 g), which led to a significant increase in the pattern cytocompatibility compared to pristine or plasma-treated FEP.

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“…The very first polymers used by the breath figure technique were star homopolymers. Thanks to the spherical shape that they form in solution, they can create highly ordered structures [ 27 ] ( Figure 3 ). However, it was later shown that even linear homopolymers can form ordered porous films [ 28 ] and their use is undoubtedly the most widespread strategy.…”

Section: Polymeric Honeycombsmentioning

confidence: 99%

“…Half a century later, dew formation, whose thermodynamic and kinetic aspects are key to understanding BF formations in polymeric materials, was studied theoretically and experimentally [ 44 ]. A breakthrough in the application of BF was achieved in 1994, when Francois et al, came up with the fabrication of polymer films with a highly ordered HCP morphology using condensed water droplets, which served as a dynamic template [ 27 ]. Star PS or polystyrene-b-polyparaphenylene dissolved in carbon sulfide was used in the experiment.…”

Section: Preparation Of Hcp Structuresmentioning

confidence: 99%

Biopolymer Honeycomb Microstructures: A Review

et al. 2023

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In this review, we present a comprehensive summary of the formation of honeycomb microstructures and their applications, which include tissue engineering, antibacterial materials, replication processes or sensors. The history of the honeycomb pattern, the first experiments, which mostly involved the breath figure procedure and the improved phase separation, the most recent approach to honeycomb pattern formation, are described in detail. Subsequent surface modifications of the pattern, which involve physical and chemical modifications and further enhancement of the surface properties, are also introduced. Different aspects influencing the polymer formation, such as the substrate influence, a particular polymer or solvent, which may significantly contribute to pattern formation, and thus influence the target structural properties, are also discussed.

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Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

Cited by 7 publications

References 54 publications

Cell Type-Specific Effects of Implant Provisional Restoration Materials on the Growth and Function of Human Fibroblasts and Osteoblasts

Cell Type-Specific Effects of Implant Provisional Restoration Materials on the Growth and Function of Human Fibroblasts and Osteoblasts

Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer

Biopolymer Honeycomb Microstructures: A Review

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