Surface plasmon resonance sensing of nucleic acids: A review

Thermodynamic and kinetic characterization of pH-dependent interactions between bovine serum albumin and ibuprofen in 2D and 3D systems

“…Contrary to the (radio)-labelled techniques in recent years a number of "label-free" techniques have been developed to report biomolecular interactions [10][11][12]. Two-dimensional SPR is a label-free technique and capable of measuring real-time quantitative binding affinities and kinetics for proteins interacting with biomolecules using relatively small (in nanomolar range) quantities of materials and has potential to be medium-throughput [13][14][15]. The conventional SPR technique requires that one binding component to be immobilised on a sensor chip while the other binding component in solution is flowed over the sensor surface; a binding interaction is detected using an optical method that measures remarkably small changes in refractive index at the sensor surface.…”

Application of Modern Biosensors Methods in Ecotoxicological Monitoring of Some Toxins of Natural (Micotoxins) and Antropogenic (Pesticides) Origin. Part 1. Micotoxins.

“…Нові тенденції у розвитку ППР-аналізу ви-світлює один з його основоположників Jiří Homola, пропонуючи використовувати досяг-нення сучасної молекулярної біології, а саме нуклеїнові кислоти (Nucleic acids, NAs), як чутливий елемент біосенсора [122].…”

Surface plasmon resonance investigation of DNA hybridization on a sensor surface using gold nanoparticles modified by specific oligonucleotides

“…Surface plasmon resonance (SPR) biosensors have become an important tool for characterizing and quantifying biomolecular interactions, because of their ability to measure biomolecular interactions directly, in real time and without the using labels [1][2][3]. However, this method, despite its many advantages, cannot ensure the detection of DNA sequences in subnanomolar concentrations without molecular label.…”

“…To improve the detection limit of the SPR biosensors, a sandwich hybridization format can be used. In this format, the procedure consists of two steps: 1) identification of a target DNA by the selective hybridization with DNA probes immobilized on the sensor surface and 2) amplification of the sensor response by the additional hybridization step between a target DNA and so-called detection probe (itself or attached to an amplification agent) [1,[3][4][5]. A key step to ensure the success of this signal amplification format is an appropriate choice of the amplification agent.…”

“…It allows us to expand the range of ionic strength of solutions in which the use of nanoparticles becomes possible. If the ionic strength of the solution is high enough to ensure the efficient DNA hybridization, and the AuNPs do not undergo aggregation, it becomes possible to use AuNPs as molecular labels to enhance the sensor signal [3][4][5]9].…”