Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

Abstract

The development of a surface plasmon resonance (SPR) spectrometer comprising angular-resolved analysis for quasi-monochromatic illumination is reported. The optical system utilizes disposable, injection-molded chips combined with a lateral imaging optical system for parallel analysis of one-dimensional spot arrays. Further parallelization is achieved by introducing a segmented light source. This source sequentially illuminates three neighbored one-dimensional arrays in order to keep angular-resolved analysis without introducing any mechanically moving parts. This system is applied to detect genetic variations among different DNA samples obtained from polymerase chain reaction (PCR). For this purpose, 135 spots on the chip surface have been prepared by spotting and analyzed separately

Biosensing platform combining label-free and labelled analysis using Bloch surface waves

“…The optical system approach is derived from a system previously developed for parallel SPR analysis on polymer chips 12 the performance of which has been demonstrated recently 20,21 . A simplified overview of the optical system modified for BSW based analysis is presented in Figure 4.…”

“…Based on our previous work on angularly resolved SPR analysis 7,12 , a new optical system has been designed and fabricated. The new system features the combination of SPR-like angularly resolved resonance and the detection of fluorescent labels.…”

Improving the sensitivity of optical biosensors by means of Bloch surface waves

“…We utilize a system established previously for parallel, label-free analysis on polymer substrates [6] as shown in image 1. It has been applied to different applications such as DNA hybridization [7] as well as protein sensing recently [8].…”

“…The optical system relies on disposable, injection moulded polymer chips and quasi monochromatic illumination. Two-dimensional images taken by a CCD camera detector yield angular resolved intensity distribution in one and lateral imaging of a one-dimensional spot array in the other coordinate [6]. This enables the comparison of angular resonance spectra.…”

“…the resonance shift upon refractive index change, has been measured using different concentrations of glucose in water. From the angular intensity distributions, as shown in image 5, the position of minimum reflectivity has been obtained by means of an adapted algorithm [6]. The results are shown in image 7.…”