Surface plasmon resonance imaging (SPRI) system and real-time monitoring of DNA biochip for human genetic mutation diagnosis of DNA amplified samples

Mannelli, Ilaria; Courtois, Virginie; Lecaruyer, Pierre; Roger, G.; Millot, Marie‐Claude; Goossens, Michel; Canva, Michael

doi:10.1016/j.snb.2006.01.023

Cited by 50 publications

(31 citation statements)

References 22 publications

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“…After having validated our setup with short synthetic DNA molecules, we applied our system to the detection of a gene mutation in analyzing PCR products [14]. The sequence of the CFTR gene is 189 kilobases long and cannot be directly sent on the biochip.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…This would diminish the systematic false negative diagnosis by a factor of three, from 15 to 5%. An enzymatically digested single-stranded PCR-amplified DNA containing exon 10 [14], obtained from a patient Fig. 12 We use 17 exons and 2 introns containing 65 mutations of the CFTR gene related to the CF disease.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…It allows quantification of the binding events, especially the quantity of bound material in quasiequilibrium states and the associated kinetic constants, or more generally the dynamics of the binding and unbinding reactions, allowing biochemical affinity measurements and comparisons. Interactions tested include DNA-DNA [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27], DNA-protein [28][29][30][31][32][33][34], protein-protein [35,36], and applications such as health safety in food [37][38][39][40][41][42][43] or even microbial detection in space [44].…”

Section: Introductionmentioning

confidence: 99%

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Plasmonic DNA: Towards Genetic Diagnosis Chips

et al. 2007

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The present paper summarizes some of our activities in the field of plasmonic DNA and genetic diagnosis, presenting our system and its capabilities before showing data related to the design and use of functionalized biochips of increasing complexity along with various experimental hybridization conditions, including solutions containing one type of purified synthetic short oligonucleotides or PCR-amplified DNA samples from patients. The diagnosis capability of our system was evaluated by detecting several point mutations that alter the function of the CFTR gene and cause cystic fibrosis, a frequent monogenic disorder selected as a clinical model system.

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Section: Polymerase Chain Reactionmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plasmonic DNA: Towards Genetic Diagnosis Chips

et al. 2007

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“…Many studies have been done to improve the SPRi performance, such as nano-structured SPR biosensor [13] and SPR phase detection techniques [14]. Various SPRi devices have been developed to implement hybridization-based sensing, including miRNA/DNA detection [15], bacterial genotyping and SNP identification [16,17]. However, their detection sensitivity is not as high as the traditional angle-interrogation SPR biosensor while maintaining high-throughput detection.…”

Section: Introductionmentioning

confidence: 99%

Biosensing of BCR/ABL fusion gene using an intensity-interrogation surface plasmon resonance imaging system

Huang

Bian

et al. 2016

Optics Communications

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a b s t r a c tIn this work, a custom-made intensity-interrogation surface plasmon resonance imaging (SPRi) system has been developed to directly detect a specific sequence of BCR/ABL fusion gene in chronic myelogenous leukemia (CML). The variation in the reflected light intensity detected from the sensor chip composed of gold islands array is proportional to the change of refractive index due to the selective hybridization of surface-bound DNA probes with target ssDNA. SPRi measurements were performed with different concentrations of synthetic target DNA sequence. The calibration curve of synthetic target sequence shows a good relationship between the concentration of synthetic target and the change of reflected light intensity. The detection limit of this SPRi measurement could approach 10.29 nM. By comparing SPRi images, the target ssDNA and non-complementary DNA sequence are able to be distinguished. This SPRi system has been applied for assay of BCR/ABL fusion gene extracted from real samples. This nucleic acidbased SPRi biosensor therefore offers an alternative high-effective, high-throughput label-free tool for DNA detection in biomedical research and molecular diagnosis.

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“…Surface plasmon resonance imaging (SPRI) has been reported [9,10] for the simultaneous detection and discrimination of different cystic fibrosis mutations including DF508 and other single nucleotide polymorphisms (SNPs) located in the same region of the CFTR gene (Exon 10). Using SPRI Mannelli et al reported on the label-less real time detection of several DNA targets (short oligonucleotides or a 377 bp PCR product) on a 196 spot DNA array [9]. The same author also reported the SPRI parallel detection of several cystic fibrosis associated mutations; DF508, DI507, M470V, Q493X, V520F and 1716 G > A [10].…”

Section: Introductionmentioning

confidence: 99%