Biosensing of BCR/ABL fusion gene using an intensity-interrogation surface plasmon resonance imaging system

Abstract

a b s t r a c tIn this work, a custom-made intensity-interrogation surface plasmon resonance imaging (SPRi) system has been developed to directly detect a specific sequence of BCR/ABL fusion gene in chronic myelogenous leukemia (CML). The variation in the reflected light intensity detected from the sensor chip composed of gold islands array is proportional to the change of refractive index due to the selective hybridization of surface-bound DNA probes with target ssDNA. SPRi measurements were performed with different concentrations of synthetic target DNA sequence. The calibration curve of synthetic target sequence shows a good relationship between the concentration of synthetic target and the change of reflected light intensity. The detection limit of this SPRi measurement could approach 10.29 nM. By comparing SPRi images, the target ssDNA and non-complementary DNA sequence are able to be distinguished. This SPRi system has been applied for assay of BCR/ABL fusion gene extracted from real samples. This nucleic acidbased SPRi biosensor therefore offers an alternative high-effective, high-throughput label-free tool for DNA detection in biomedical research and molecular diagnosis.

Plasmonic molecular assays: Recent advances and applications for mobile health

“…The two single-stranded products were then annealed to form double-stranded detection sequences with a toehold that were captured and detected by oligofunctionalized SPR sensor. Ding et al developed an intensity-based SPR imaging (SPRi) system combining DNA microarray technology for quantification of denatured PCR products [113]. This system has been used for detection of the BCR/ABL fusion gene in chronic myelogenous leukemia (CML) with a LOD of 10.29 nM.…”

“…However, one potential limitation of plasmonic thin film is that the heating range is limited to approximately 100 nm from the gold surface, limiting its applications toward bulk solutions. This however has been improved later by using two layers of plasmonic films to form an optical cavity as thick as 750 μm, within which the PCR mixture experienced a more uniform temperature distribution across the cavity [113]. By optimizing the film thickness of top and bottom plasmonic films, ultrafast 30 thermal cycles from 68 (annealing/extension) to 94 °C (denaturation) were achieved within 4-10 min for reaction volume from 1.3-10 μL.…”

DNA Probe as Biosensor Candidate for Clavibacter michiganensis subsp. michiganensis on Tomato Plants

“…detection techniques because its accuracy and sensitivity, especially when the concentration of the target is low (Wu et al 2019). Many experiments have been reported to improve the efficiency and accuracy of this method, including probe selection and the optimization of probe concentrations (Wu et al 2016).…”

Detection of carcinoembryonic antigens using a wavy gold–silver alloy nanoplate enhanced surface plasmon resonance imaging biosensor

“…SPRi measurements were performed on a custom-made apparatus with a slight improvement to investigate the interaction of proteins, which had been successfully used. 22 This optical setup was designed as illustrated in Fig. 1.…”

“…The gold nanoisland microchip was prepared with a method reported previously. 22 BK7 glass slides were cleaned in a piranha solution and then washed with deionized water and ethanol, and dried by using nitrogen. Thus, e-beam lithography was employed to fabricate gold cylinders on these glass substrates.…”