Surface plasmon resonance imaging for ABH antigen detection on red blood cells and in saliva: secretor status-related ABO subgroup identification

Abstract

Low antigenic expression of ABO subgroup system on red blood cell (RBC) is cause of discrepancy between forward and reverse blood typing in the standard agglutination technique. Neutralization agglutination is employed for verification of the detection of ABH substances in saliva. However, the neutralization technique is complicated, time-consuming and requires expertise. To overcome these drawbacks, surface plasmon resonance (SPR) imaging was developed for ABH antigen detection on RBCs and in saliva. An antibody array was designed to classify the ABO subgroups by anti-A, anti-B, and anti-H antibodies; the array was immobilized on a carboxymethyl-dextran sensor-surface. RBCs and saliva specimens from sixty-four donors were analysed by passing them over the antibody array, where the secretor status and blood group could be simultaneously identified. Consequently, the immobilized antibodies could specifically and quantitatively detect the ABH antigen on RBCs. Using the direct assay, the SPR signal of saliva detection was weaker than that of RBC detection. However, a sandwich assay with a mixture of anti-A, anti-B, and anti-H antibodies could efficiently enhance the signal. The sensor chip provided high specificity (cut-off at 100 to 175 micro refractive index units) and high precision at 0.06%-4.9% CV. The blood group results of the sixty-four donor specimens obtained by SPR agreed with the standard agglutination test with 100% accuracy. SPR could indicate different ABH antigen densities on the RBCs and nearly the same amounts of ABH substances in the saliva of strong and weak subgroups. Finally, we also demonstrated reduced assay time and fewer complications with the SPR imaging platform compared to the neutralization technique.

Dental Radiographic/Digital Radiography Technology along with Biological Agents in Human Identification

“…Orthopantomography or panoramic radiographs are the panoramic X-ray scanning of the upper and lower jaws that provide a broad radiologic graph from the human dentition. Because of the low radiation dose and short time required for imaging, dental panoramic radiography [ 70 ] has become a broadly applied standard method in dentistry as complementary information and initial examinations for oncological treatments [ 71 – 73 ]. Accordingly, orthopantomography is now practically used for victim identification in several MDs including war casualties [ 74 ].…”

Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

“…Peungthum et al have combined SPR imaging with antibody array method for the quantitative detection of ABH antigens in saliva (Peungthum et al 2017 ). As given in the Fig.…”

“… 2019 [Derruau et al 2019 ] _ 4 AAS Release of nickel, chromium, and zinc in saliva; Use of orthodontic appliances 2019 [Quadras et al 2019 ] _ 5 SERS Effects of low-dose irradiation on saliva 2019 [Colceriu-Șimon et al 2019 ] _ 6 LIBS Calcium in saliva 2019 [Zahroh et al 2019 ] _ 7 IR Saliva composition 2018 [Bel’skaya et al 2018 ] _ 8 ATR-FTIR Forensic, body fluids, including Saliva 2018 [Takamura et al 2018 ] _ 9 SPR ABH antigen detection in saliva. 2017 [Peungthum et al 2017 ] _ 10 LSPR Wine astringency. Interaction of red wine and saliva 2017 [Guerreiro et al 2017 ] L.O.D : 1 μmol/L 11 LSPR Monitoring the pH of saliva 2017 [Luo et al 2017 ] wavelengths of 665 nm and 785 nm—the sensitivities were 0.0299 a.u./pH (a.u.…”

Low-Fouling Substrates for Plasmonic Sensing of Circulating Biomarkers in Biological Fluids

“…No other antifouling strategy besides a pretreatment of saliva samples that included freezing and boiling was carried out to inactivate salivary enzymes and prevent their adsorption. Another singular approach involving the same carboxymethyldextran-modified surface but making use of an SPR imaging biosensor was applied to detect ABH antigens from red blood cell (RBC) membranes in saliva [94]. The aim of the study was to prevent misinterpretation of blood group typing by diminishing the time of conventional agglutination assays based on standard neutralization testing.…”