Surface plasmon resonance biosensor for genetically modified organisms detection

Mariotti, Elisa; Minunni, Maria; Mascini, Marco

doi:10.1016/s0003-2670(01)01458-1

Cited by 99 publications

(47 citation statements)

References 11 publications

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“…The change in frequency signals before and after monomer incubation was recorded. For SPR measurements, the surface cleaning and modification procedures were adapted from the detailed study of Mariotti et al 25 For the EDC/NHS activation of the MUA-modified surface, the solution was flown at 5 mL min À1 for 7 min followed by the addition of fibril seeds for 30 min at a flow rate of 3 mL min…”

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confidence: 99%

Label-free methods for probing the interaction of clioquinol with amyloid-β

et al. 2012

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The presence of amyloid-b (Ab) fibrils is characteristic of Alzheimer's disease (AD), and the aggregation of these amyloidogenic proteins is a nucleation-dependent process. In this report, label-free methods based on surface plasmon resonance (SPR) and thickness shear mode acoustic wave sensors (TSM-AWS) were used to detect monomer elongation in real-time. The modulation of Ab aggregation using a well-described flavonoid, clioquinol (CQ) was also observed. Established methods like fluorescence and electrochemistry were also employed to confirm the interaction of CQ with Ab. Good correlation between the designed label-free methods creates a promising platform for the screening of novel amyloid inhibitors.

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confidence: 99%

Label-free methods for probing the interaction of clioquinol with amyloid-β

et al. 2012

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“…Many DNA devices [1][2][3], such as DNA chips, DNA sensors, and DNA computers, are based on the principle of the hybridization of ssDNA, immobilized on a substrate, to the complementary strand, the detection target. Many techniques could be used to analyze DNA interactions, such as optical detection of fluorescent oligonucleotides [4], application of surface plasma resonance spectroscopy [5], and direct electrochemical assay of double-stranded DNA [6]. Quartz crystal microbalances (QCM) are well known and very sensitive mass-measuring devices that can be readily used to monitor DNA hybridization and to study their dynamic mechanisms; QCM is an important technique that was recently developed for DNA sensing [7,8].…”

Section: Introductionmentioning

confidence: 99%

Polystyrene spheres coated with gold nanoparticles for detection of DNA

Xia

Zhang

et al. 2010

Electrophoresis

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A novel method has been developed here utilizing gold-modified polystyrene spheres (PS@Au) as carriers for the investigation of DNA hybridization. The immobilization ability of DNA probes on the PS@Au was investigated by a quartz crystal microbalance (QCM). More gold nanoparticles (AuNPs) were conjugated to the QCM surface in the form of PS@Au as compared with the AuNPs alone. The influence of PS, AuNPs population and size on the DNA probe fixation, and hybridization has been investigated. The results show that more AuNPs were achievable onto the QCM surface and could greatly increase the immobilization amount further hybridization amount of DNA. There was an optimal surface curvature for the probe DNA absorption. The curvature about 12 nm was more suited to the adsorption of probe DNA which could lead to the detection limit further down to 10(-12) M. These results demonstrate that the introduction of PS@Au, with polystyrene spheres as the medium between the QCM surface and the AuNPs, could achieve modified QCM surfaces and increase its detection ability for a better DNA assay and a novel platform for the fabrication of DNA sensors.

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“…The gene is able to express an additional protein that confers new characteristics, i.e. herbicide tolerance or resistance to virus, antibiotic and insect (Niederhauser et al, 1996;Droge et al, 1998;Vollenhofer et al, 1999;Hails, 2000;Minunni et al, 2001;Mariotti et al, 2002). The foreign DNA is usually inserted into a gene 'cassette' consisting of an expression promoter (P), a structural gene (encoding region) and an expression terminator (T).…”

Section: Introductionmentioning

confidence: 99%