Surface plasmon resonance biosensor for the detection of phenylethanolamine A in swine urine

Fan, Sufang; Li, Chunsheng; Ma, Junmei; Meng, Zhijuan; Zhao, Limin; Zhang, Yan

doi:10.1039/d1ay00132a

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…After eating the foods containing the residue of this drug, people will appear nausea, dizziness, limb weakness, hand tremor and other poisoning symptoms, long-term consumption will even induce malignant tumors, so it is prohibited to use in feed and animal drinking water. At present, the methods for the detection of phenethanolamine A reported in the literature mainly include liquid chromatoc-series mass spectrometry [1,2], enzyme-linked immunoassay [3,4], biosensor method [5], colloidal gold strip method [6], etc. The substrates for the detection of phenethanolamine A residue reported in the literature mainly include hair of Swine and Sheep [7], pork [8,9], pig urine [10] and feed ISSN: 2688-8645 2 [11].…”

Section: Introductionmentioning

confidence: 99%

Determination of Phenylethanolamine A Residues in Milk by Solid Phase Extraction-Liquid Chromatography Tandem Mass Spectrometry

Ni,

Li,

Zhang

et al. 2023

SJT

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A method was established for the determination of phenethanolamine A residue in milk by solid phase extraction, liquid chromatography-tandem mass spectrometry. The samples were extracted with ammonium acetate buffer solution under the condition of enzymatic hydrolysis and purified by MCX solid phase extraction column. The eluent was blown dry with nitrogen at 45℃ and dissolved with 1.0 mL 0.1% formic acid solution. The samples were isolated by Poroshell 120EC-C18, 100 mm ×3.0 mm (i.d.), 2.7 μm column, and then determined by mass spectrometry in multiple reaction monitoring mode, and quantified by internal standard method. The results showed that phenethanolamine A had good linear relationship in the concentration range of 0.5-50.0 μg/L, the correlation coefficient r=0.9984, the limit of detection was 0.17 μg/kg, and the limit of quantitation was 0.57 μg/kg. The average recovery was 98.3-108.7% and the precision was 2.4-3.7% at different supplemental levels. The established method can purify the samples effectively, improve the detection efficiency, and is suitable for the detection of phenethanolamine A residue in milk.

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Section: Introductionmentioning

confidence: 99%

Determination of Phenylethanolamine A Residues in Milk by Solid Phase Extraction-Liquid Chromatography Tandem Mass Spectrometry

Ni,

Li,

Zhang

et al. 2023

SJT

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“…9 Despite of the use of β-agonists mainly in livestock for nutrition repartitioning, most of the drug is excreted through urine. 10 Moreover, they can be flushed off and introduced to the surrounding environment through rainfall, runoff, wastewater, and manure disposal. 11−13 Thus, the neighboring groundwater, streams, and rivers are possibly contaminated, as reported for the detected residue of β-agonists.…”

Section: ■ Introductionmentioning

confidence: 99%

Determination of 18 β-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry

Han

Chen

Yang

et al. 2022

ACS Food Sci. Technol.

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An effective and efficient method for the determination of 18 β-agonists in aquatic products using liquid chromatography tandem mass spectrometry (LC-MS/MS) was successfully established. The method optimization was conducted based on incurred samples to guarantee its practicability. In this method, the sample was hydrolyzed with β-glucuronidase-arylsulfatase in ammonium acetate solution and treated successively with protein precipitation in 5% trichloroacetic acid in water, lipid removal with n-hexane, and further concentration and cleanup by solid-phase extraction (hydrophilic–lipophilic balanced cartridge, primary–secondary amine cartridge). Isotope-labeled internal standards and matrix-matched calibration curves were used for quantification. The limit of quantification (LOQ) was verified at 0.5–1.0 μg/kg (S/N > 10). This method was evaluated with five different matrices, including eel, roasted eel, pufferfish, shrimp, and crab, at 1×, 2×, and 10× LOQs, with good recoveries (>77%) and high replicate repeatability (relative standard deviations (RSDs) <13%, intraday batch repeatability) and further validated by three other accredited laboratories. A total of 3 samples out of 37 tested aquatic products were found positive for unknown incur sources (ractopamine, 0.38–2.1 μg/kg; salbutamol 3.0 μg/kg; tulobuterol 2.9 μg/kg; clenbuterol 1.3 μg/kg; and clenpenterol, 0.3 μg/kg). The method has demonstrated to be reliable, sensitive, and stable for multiresidue screening of β-agonists for food safety monitoring.

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Nanosize Controllable Two‐Dimensional (Cu–S)_n Metal–Organic Framework Hybrid With Reduced Graphene Oxide by In Situ Microwave and Hydrothermal Synthesis for Electrochemical Neurotransmitter Detection

Lin,

Yu,

et al. 2024

Applied Organom Chemis

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We present a groundbreaking technique for controllably synthesizing nanosized semiconductive two‐dimensional (2D) (Cu–S)n metal–organic framework (MOF)/graphene nanocomposites for electrochemical sensing of neurotransmitter 2‐phenethylamine (PEA). The (Cu–S)n MOF size is controlled by graphene oxide proportions, enhancing electrocatalytic activity. The modified electrode exhibits a surface area increase from 0.0639 to 0.1906 cm2 and impressive limit of detection (0.0156 and 0.004 μM) within linear ranges of 15–200 μM and 1–10 μM, offering exceptional selectivity, reproducibility, and repeatability for biosensing.

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Surface plasmon resonance biosensor for the detection of phenylethanolamine A in swine urine

Abstract: In the present study, an antibody against phenylethanolamine A (PEA) was produced, confirmed, and used in a surface plasmon resonance (SPR)-based measurement. Bovine serum albumin (BSA)-conjugated PEA was linked to...

Cited by 5 publications

References 32 publications

Determination of Phenylethanolamine A Residues in Milk by Solid Phase Extraction-Liquid Chromatography Tandem Mass Spectrometry

Determination of Phenylethanolamine A Residues in Milk by Solid Phase Extraction-Liquid Chromatography Tandem Mass Spectrometry

Determination of 18 β-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry

Nanosize Controllable Two‐Dimensional (Cu–S)_n Metal–Organic Framework Hybrid With Reduced Graphene Oxide by In Situ Microwave and Hydrothermal Synthesis for Electrochemical Neurotransmitter Detection

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Surface plasmon resonance biosensor for the detection of phenylethanolamine A in swine urine

Abstract: In the present study, an antibody against phenylethanolamine A (PEA) was produced, confirmed, and used in a surface plasmon resonance (SPR)-based measurement. Bovine serum albumin (BSA)-conjugated PEA was linked to...

Cited by 5 publications

References 32 publications

Determination of Phenylethanolamine A Residues in Milk by Solid Phase Extraction-Liquid Chromatography Tandem Mass Spectrometry

Determination of Phenylethanolamine A Residues in Milk by Solid Phase Extraction-Liquid Chromatography Tandem Mass Spectrometry

Determination of 18 β-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry

Nanosize Controllable Two‐Dimensional (Cu–S)n Metal–Organic Framework Hybrid With Reduced Graphene Oxide by In Situ Microwave and Hydrothermal Synthesis for Electrochemical Neurotransmitter Detection

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Nanosize Controllable Two‐Dimensional (Cu–S)_n Metal–Organic Framework Hybrid With Reduced Graphene Oxide by In Situ Microwave and Hydrothermal Synthesis for Electrochemical Neurotransmitter Detection