Surface plasmon resonance biosensor based on engineered proteins for direct detection of interferon-gamma in diluted blood plasma

Šípová, Hana; Ševců, V.; Kuchař, Milan; Ahmad, Jawid Nazir; Mikulecký, Pavel; Osička, Radim; Malý, Petr; Homola, Jiřı́

doi:10.1016/j.snb.2012.08.024

Cited by 28 publications

(9 citation statements)

References 23 publications

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“…The sensor is based on the attenuated total reflection method and wavelength spectroscopy of surface plasmons (33). In this sensor, white light is used to excite surface plasmons on a thin gold film in four areas, which are interfaced with four separate chambers of a flow cell (34).…”

Section: Methodsmentioning

confidence: 99%

5′-O-Methylphosphonate nucleic acids—new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

Šípová

Špringer

Rejman

et al. 2014

Nucleic Acids Research

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Several oligothymidylates containing various ratios of phosphodiester and isopolar 5′-hydroxyphosphonate, 5′-O-methylphosphonate and 3′-O-methylphosphonate internucleotide linkages were examined with respect to their hybridization properties with oligoriboadenylates and their ability to induce RNA cleavage by ribonuclease H (RNase H). The results demonstrated that the increasing number of 5′-hydroxyphosphonate or 5′-O-methylphosphonate units in antisense oligonucleotides (AOs) significantly stabilizes the heteroduplexes, whereas 3′-O-methylphosphonate AOs cause strong destabilization of the heteroduplexes. Only the heteroduplexes with 5′-O-methylphosphonate units in the antisense strand exhibited a significant increase in Escherichia coli RNase H cleavage activity by up to 3-fold (depending on the ratio of phosphodiester and phosphonate linkages) in comparison with the natural heteroduplex. A similar increase in RNase H cleavage activity was also observed for heteroduplexes composed of miRNA191 and complementary AOs containing 5′-O-methylphosphonate units. We propose for this type of AOs, working via the RNase H mechanism, the abbreviation MEPNA (MEthylPhosphonate Nucleic Acid).

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Section: Methodsmentioning

confidence: 99%

5′-O-Methylphosphonate nucleic acids—new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

Šípová

Špringer

Rejman

et al. 2014

Nucleic Acids Research

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show abstract

“…Beyond these strategies such as novel antibiofouling coatings and the addition of tags for additional mass to improve sensitivity, novel scaffolds derived from albumin binding domains (ABD) of protein G were also engineered for the detection of IFN-γ as superior biorecognition elements relative to primary capture antibodies [119]. Via covalent functionalization of a SPR sensor surface with the ABD scaffolds, a DL of 3.38 ng/mL (0.2 nM) was attained for IFN-γ in 2% depleted human blood plasma [120]. Another improvement approach includes sample purification ahead of SPR measurement.…”

Section: Cytokine Sensors With Various Optical Modes Of Detectionmentioning

confidence: 99%

Emerging Cytokine Biosensors with Optical Detection Modalities and Nanomaterial-Enabled Signal Enhancement

Singh

Truong

Reeves

et al. 2017

Sensors

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Protein biomarkers, especially cytokines, play a pivotal role in the diagnosis and treatment of a wide spectrum of diseases. Therefore, a critical need for advanced cytokine sensors has been rapidly growing and will continue to expand to promote clinical testing, new biomarker development, and disease studies. In particular, sensors employing transduction principles of various optical modalities have emerged as the most common means of detection. In typical cytokine assays which are based on the binding affinities between the analytes of cytokines and their specific antibodies, optical schemes represent the most widely used mechanisms, with some serving as the gold standard against which all existing and new sensors are benchmarked. With recent advancements in nanoscience and nanotechnology, many of the recently emerging technologies for cytokine detection exploit various forms of nanomaterials for improved sensing capabilities. Nanomaterials have been demonstrated to exhibit exceptional optical properties unique to their reduced dimensionality. Novel sensing approaches based on the newly identified properties of nanomaterials have shown drastically improved performances in both the qualitative and quantitative analyses of cytokines. This article brings together the fundamentals in the literature that are central to different optical modalities developed for cytokine detection. Recent advancements in the applications of novel technologies are also discussed in terms of those that enable highly sensitive and multiplexed cytokine quantification spanning a wide dynamic range. For each highlighted optical technique, its current detection capabilities as well as associated challenges are discussed. Lastly, an outlook for nanomaterial-based cytokine sensors is provided from the perspective of optimizing the technologies for sensitivity and multiplexity as well as promoting widespread adaptations of the emerging optical techniques by lowering high thresholds currently present in the new approaches.

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“…Numerous radio-labelled and "label-free" equilibrium or time-resolved analysis techniques are available which able to monitor and quantify protein-ligand bindings. The SPR spectroscopy is one of the most useful quasi two-dimensional technique which is able to measure real-time association and dissociation processes between DNA-DNA, DNA-protein, lipid-protein and protein-or peptides-drug molecules in a label free environment [3,4]. While one of the interacting partners is immobilized onto the sensor surface, the other is flow in solution over the surface or exist as bound (surface complex) form.…”

Section: Introductionmentioning

confidence: 99%

Modelling and Characterization of the Sorption of Kynurenic Acid on Protein Surfaces

Juhász

Csapó

Vécsei

et al. 2017

Period. Polytech. Chem. Eng.

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Surface plasmon resonance biosensor based on engineered proteins for direct detection of interferon-gamma in diluted blood plasma

Cited by 28 publications

References 23 publications

5′-O-Methylphosphonate nucleic acids—new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

5′-O-Methylphosphonate nucleic acids—new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

Emerging Cytokine Biosensors with Optical Detection Modalities and Nanomaterial-Enabled Signal Enhancement

Modelling and Characterization of the Sorption of Kynurenic Acid on Protein Surfaces

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