Surface plasmon resonance-based aptasensor for direct monitoring of thrombin in a minimally processed human blood

Kotlarek, Daria; Curti, Federica; Vorobii, Mariia; Corradini, Roberto; Careri, Maria; Knoll, Wolfgang; Rodriguez‐Emmenegger, Cesar; Dostálek, Jakub

doi:10.1016/j.snb.2020.128380

Cited by 35 publications

(44 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[7,8] Thus, the practical application of bioactive surfaces requires antifouling layers capable of preventing protein and cell fouling from complex biological media. [9,10] Antifouling coatings can be generated by immobilizing self-assembled monolayers (SAMs) or "grafted-to" or "graftedfrom" polymer coatings on a surface. [6,[11][12][13][14] SAMs are broadly applied to introduce different functionalities to the surfaces.…”

mentioning

confidence: 99%

Diblock and Random Antifouling Bioactive Polymer Brushes on Gold Surfaces by Visible‐Light‐Induced Polymerization (SI‐PET‐RAFT) in Water

Kuzmyn

Teunissen

Fritz

et al. 2021

Adv Materials Inter

View full text Add to dashboard Cite

that perform certain biological functions, such as specific cell adsorption or interaction with a specific protein or analyte. [3] As part of a device, bioactive surfaces are commonly required to perform in complex biological media such as blood, saliva, or urine. [1,[4][5][6] These fluids typically contain numerous types of proteins and cells that may interfere with the performance of the bioactive surface by nonspecific adsorption. Such nonspecific adsorption by proteins and cells from biological media on a surface is called fouling. [7,8] Thus, the practical application of bioactive surfaces requires antifouling layers capable of preventing protein and cell fouling from complex biological media. [9,10] Antifouling coatings can be generated by immobilizing self-assembled monolayers (SAMs) or "grafted-to" or "graftedfrom" polymer coatings on a surface. [6,[11][12][13][14] SAMs are broadly applied to introduce different functionalities to the surfaces. [15,16] Notably, oligo(ethylene glycol)-terminated [4] and zwitterionic SAMs [17] can resist or decrease fouling from single-protein solutions and cell cultures. "Grafted-to" polymers based on ethylene glycol oxide, oxazoline, [18] N-(2-hydroxypropyl) methacrylamide (HPMA), [12] and zwitterionic moieties [19] have shown good resistance toward single-protein solutions and moderate-to-good resistance toward more complex biological media. The "grafted-from" approach, in which polymer chains are grown from a surface, allows the Surface-initiated photoinduced electron-transfer-reversible addition-fragmentation chain transfer (SI-PET-RAFT) is, for the first time, used for the creation of antifouling polymer brushes on gold surfaces based on three monomers: oligo(ethylene glycol) methyl ether methacrylate (MeOEGMA), N-(2-hydroxypropyl) methacrylamide (HPMA), and carboxybetaine methacrylamide (CBMA). These coatings are subsequently characterized by X-ray photoelectron spectroscopy (XPS) and ellipsometry. The living nature of this polymerization allows for the creation of random and diblock copolymer brushes, which are based on HPMA (superb antifouling) and CBMA (good antifouling and functionalizable via activated ester chemistry). The polymer brushes demonstrate good antifouling properties against undiluted human serum, as monitored by quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR) spectroscopy in real time. The amount of immobilization of bioactive moieties, here an antibody immobilized using N-succinimidyl ester-1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (NHS-EDC) coupling, in the diblock and random copolymer brushes is monitored by SPR, and is analyzed with respect to the brush structure, and is shown to be superior in the diblock copolymer brush. This approach represents a scalable, robust, mild, oxygen-tolerant, and heavy-metal-free route toward the production of antifouling and functional copolymer brushes (on gold surfaces) that open up applications in biosensing and tissue engineering.

show abstract

mentioning

confidence: 99%

Diblock and Random Antifouling Bioactive Polymer Brushes on Gold Surfaces by Visible‐Light‐Induced Polymerization (SI‐PET‐RAFT) in Water

Kuzmyn

Teunissen

Fritz

et al. 2021

Adv Materials Inter

View full text Add to dashboard Cite

show abstract

“…Notably most small molecules bind aptamers only with the one-site binding configuration because there is no room for the aptamer to interact with a second molecule [60]. Therefore, many SPR aptasensors have been developed based on this direct strategy [61][62][63][64][65]. For example Wu et al and Ashley et al immobilized a biotin-modified aptamer probe on an avidin modified chip through streptavidin-biotin interaction for the detection of aflatoxin in vin egar and lysozyme in milk [66,67].…”

Section: Basic Spr Assaymentioning

confidence: 99%

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang

2021

Biosensors

View full text Add to dashboard Cite

Surface plasmon resonance (SPR) can track molecular interactions in real time, and is a powerful as well as widely used biological and chemical sensing technique. Among the different SPR-based sensing applications, aptamer-based SPR biosensors have attracted significant attention because of their simplicity, feasibility, and low cost for target detection. Continuous developments in SPR aptasensing research have led to the emergence of abundant technical and design concepts. To understand the recent advances in SPR for biosensing, this paper reviews SPR-based research from the last seven years based on different sensing-type strategies and sub-directions. The characteristics of various SPR-based applications are introduced. We hope that this review will guide the development of SPR aptamer sensors for healthcare.

show abstract

“…Lately, the same antifouling polymer brush (poly [HPMA-co-CBMAA]) was synthesized on a gold surface via photoinduced single-electron transfer living radical polymerization and then functionalized by including three aptamers for specific direct detection of two particular binding sites of thrombin (exosite I and II) in minimally processed 10% blood. This brush architecture exhibited a limit of detection with HD1 aptamer equal to 0.7 nM, adequate for predicting a thrombotic event and diagnosis of thrombosis [118].…”

Section: Antifouling Strategies For Spr Biosensing In Clinical Diagno...mentioning

confidence: 99%

Recent Advances in Antifouling Materials for Surface Plasmon Resonance Biosensing in Clinical Diagnostics and Food Safety

et al. 2021

View full text Add to dashboard Cite

Strategies to develop antifouling surface coatings are crucial for surface plasmon resonance (SPR) sensing in many analytical application fields, such as detecting human disease biomarkers for clinical diagnostics and monitoring foodborne pathogens and toxins involved in food quality control. In this review, firstly, we provide a brief discussion with considerations about the importance of adopting appropriate antifouling materials for achieving excellent performances in biosensing for food safety and clinical diagnosis. Secondly, a non-exhaustive landscape of polymeric layers is given in the context of surface modification and the mechanism of fouling resistance. Finally, we present an overview of some selected developments in SPR sensing, emphasizing applications of antifouling materials and progress to overcome the challenges related to the detection of targets in complex matrices relevant for diagnosis and food biosensing.

show abstract

Surface plasmon resonance-based aptasensor for direct monitoring of thrombin in a minimally processed human blood

Cited by 35 publications

References 42 publications

Diblock and Random Antifouling Bioactive Polymer Brushes on Gold Surfaces by Visible‐Light‐Induced Polymerization (SI‐PET‐RAFT) in Water

Diblock and Random Antifouling Bioactive Polymer Brushes on Gold Surfaces by Visible‐Light‐Induced Polymerization (SI‐PET‐RAFT) in Water

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Recent Advances in Antifouling Materials for Surface Plasmon Resonance Biosensing in Clinical Diagnostics and Food Safety

Contact Info

Product

Resources

About