Surface Plasma Treatment of Poly(caprolactone) Micro, Nano, and Multiscale Fibrous Scaffolds for Enhanced Osteoconductivity

Sankar, Deepthi; Shalumon, K.T.; Chennazhi, K.P.; Menon, Deepthy; Jayakumar, R.

doi:10.1089/ten.tea.2013.0569

Cited by 54 publications

(40 citation statements)

References 49 publications

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“…The differentiation of hMSCs towards osteogenic lineage was evaluated by quantifying the alkaline phosphatase (ALP) activity. An enhanced differentiation was identified on plasma-treated fibers and was further confirmed by the mineralization (alizarin red staining) of the treated scaffolds, revealing the maturation of the differentiated osteoblasts [150]. In a recent study conducted by Jeon et al plasma technology was used to generate nanosized patterns on electrospun PCL microfibers and to subsequently study the behavior of osteoblast-like-cells (MG63).…”

Section: Pcl Fibersmentioning

confidence: 94%

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Ghobeira¹,

Morent²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Section: Pcl Fibersmentioning

confidence: 94%

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Ghobeira¹,

Morent²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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“…Several methods have been employed to enhance the characteristics of hydrophobic polymers, including plasma treatment, surface modification and polymer coating . However, although immediately beneficial, these do not address the issue that PCL is biodegradable.…”

Section: Introductionmentioning

confidence: 99%

Use of lecithin to control fiber morphology in electrospun poly (ɛ‐caprolactone) scaffolds for improved tissue engineering applications

Coverdale

Gough

Sampson

et al. 2017

J Biomedical Materials Res

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We elucidate the effects of incorporating surfactants into electrospun poly (ɛ‐caprolactone) (PCL) scaffolds on network homogeneity, cellular adherence and osteogenic differentiation. Lecithin was added with a range of concentrations to PCL solutions, which were electrospun to yield functionalized scaffolds. Addition of lecithin yielded a dose‐dependent reduction in scaffold hydrophobicity, whilst reducing fiber width and hence increasing specific surface area. These changes in scaffold morphology were associated with increased cellular attachment of Saos‐2 osteoblasts 3‐h postseeding. Furthermore, cells on scaffolds showed comparable proliferation over 14 days of incubation to TCP controls. Through model‐based interpretation of image analysis combined with gravimetric estimates of porosity, lecithin is shown to reduce scaffold porosity and mean pore size. Additionally, lecithin incorporation is found to reduce fiber curvature, resulting in increased scaffold specific elastic modulus. Low concentrations of lecithin were found to induce upregulation of several genes associated with osteogenesis in primary mesenchymal stem cells. The results demonstrate that functionalization of electrospun PCL scaffolds with lecithin can increase the biocompatibility and regenerative potential of these networks for bone tissue engineering applications. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2865–2874, 2017.

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“…Generally, nonwoven polymer fibrous scaffolds are made with spinning techniques either using polymer solutions or melts . The primary focus of the spinning technique is the production of fibers in a scale range from nano‐ to microrange that resembles the native extracellular matrix (ECM) . Although there has been much research on solution spinning to form fibrous polymer scaffolds for tissue engineering and wound healing applications, little has been reported on melt spinning to fabricate nonwoven scaffolds .…”

Section: Introductionmentioning

confidence: 99%

Making Nonwoven Fibrous Poly(ε‐caprolactone) Constructs for Antimicrobial and Tissue Engineering Applications by Pressurized Melt Gyration

Mahalingam

Basnett

et al. 2016

Macro Materials & Eng

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scaffolds are made with spinning techniques either using polymer solutions or melts. [ 3,4 ] The primary focus of the spinning technique is the production of fi bers in a scale range from nano-to microrange that resembles the native extracellular matrix (ECM). [5][6][7] Although there has been much research on solution spinning to form fi brous polymer scaffolds for tissue engineering and wound healing applications, little has been reported on melt spinning to fabricate nonwoven scaffolds. [ 8,9 ] Melt spinning does not require solvents that are mostly cytotoxic, therefore it offers a distinct advantage. In addition, the surface topography of the fi brous scaffolds which can affect cellular infi ltration can be better controlled by melt spinning. [ 8,10 ] Poly(ε-caprolactone) (PCL) is one of the most promising linear aliphatic polyesters used extensively in the biomedical field since it is biodegradable in an aqueous medium and biocompatible in biological applications. This semi-crystalline polymer has a low melting point (60 °C) and a glass transition temperature (−60 °C) and therefore it could be fabricated easily into any shape and size. [11][12][13] The superior rheo logical properties and mechanical properties of PCL have A pressurized melt gyration process has been used for the fi rst time to generate poly(ε-caprolactone) (PCL) fi bers. Gyration speed, working pressure, and melt temperature are varied and these parameters infl uence the fi ber diameter and the temperature enabled changing the surface morphology of the fi bers. Two types of nonwoven PCL fi ber constructs are prepared. First, Ag-doped PCL is studied for antibacterial activity using Gram-negative Escherichia coli and Pseudo monas aeruginosa microorganisms. The melt temperature used to make these constructs signifi cantly infl uences antibacterial activity. Neat PCL nonwoven scaffolds are also prepared and their potential for application in muscular tissue engineering is studied with myoblast cells. Results show signifi cant cell attachment, growth, and proliferation of cells on the scaffolds.

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Surface Plasma Treatment of Poly(caprolactone) Micro, Nano, and Multiscale Fibrous Scaffolds for Enhanced Osteoconductivity

Cited by 54 publications

References 49 publications

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Use of lecithin to control fiber morphology in electrospun poly (ɛ‐caprolactone) scaffolds for improved tissue engineering applications

Making Nonwoven Fibrous Poly(ε‐caprolactone) Constructs for Antimicrobial and Tissue Engineering Applications by Pressurized Melt Gyration

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