Surface phenotype analysis of CD16+ monocytes from leukapheresis collections for peripheral blood progenitors

Abstract

SUMMARYIn peripheral blood progenitor cell (PBPC) collections from patients with solid tumour or haematological malignancy, monocytes were separated into two subpopulations. The majority of monocytes expressed CD14 at a high density without CD16 antigen (the CD14 þ CD16 ¹ monocytes). The remaining monocytes co-expressed CD14 and CD16 (the CD14þ monocytes amounted to 20·6 Ϯ 15·8%, while those in peripheral blood (PB) obtained from healthy volunteers were 7·3 Ϯ 3·1% (P < 0·05 CD16þ monocytes in which the proportion of the immature population is increased. It is likely that these monocytes participate in the haematological and immune recovery after PBPC transplantation.

Immature monocytes from G-CSF-mobilized peripheral blood stem cell collections carry surface-bound IL-10 and have the potential to modulate alloreactivity

“…The LPS receptor (CD14) is essential in the control of monocyte function, as it initiates many signaling cascades inside the cell [24]. As CD14 is not highly expressed on a large proportion of gmPB monocytes, they may respond poorly to LPS stimulation, and thus, LPS may not be the most effective factor for use in activation studies [25]. Recently, synergistic LPS signaling has been described through CD14 interaction with TLR4 [26].…”

The contribution of monocyte infection and trafficking to viral persistence, and maintenance of the viral reservoir in HIV infection

“…CD14loCD16hi monocytes also have phagocytic capacity [70], although perhaps lower than that of CD14hi monocytes [60]. This CD14loCD16hi subset expands (to greater than 20Ϫ40% of circulating monocytes) in response to inflammation [60] and malignancy [71]. Their expansion in peripheral blood may result from mobilization from the marginal pool [72].…”

Cytokine Production Is Altered in Monocytes from Children with Hemolytic Uremic Syndrome

“…About 90% of peripheral blood Mo consists of cells CD14+ CD16− (usually considered as "classical Mo"). Although the remaining Mo express CD16, they also present heterogeneity in CD14 expression [33,34]. CD14dim CD16+ Mo are regarded as pro-inflammatory because upon LPS stimulation they produce more TNF-α than classical Mo and little, if any, IL-10 [35,36].…”