Immature monocytes from G-CSF-mobilized peripheral blood stem cell collections carry surface-bound IL-10 and have the potential to modulate alloreactivity

Fraser, Alasdair R.; Cook, Gordon; Franklin, I. M.; Templeton, J. G. C.; Campbell, Morag H.; Holyoake, Tessa L.; Campbell, John

doi:10.1189/jlb.0605297

Cited by 27 publications

(24 citation statements)

References 32 publications

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“…22 However, elevated serum IL-12 levels may also enhance the GVL reaction, because they were shown to be associated with an improved relapse-free survival. 23 Our findings provide evidence that, in addition to the tolerogenic effects of G-CSF described by others, [5][6][7][8][9][10][11] proinflammatory slanDCs in PBSC grafts are increased in numbers and are functionally competent. Thus, grafts containing slanDCs that are infused directly after conditioning therapy can be important for the immediate inflammatory response and for stimulating indirect T-cell immune responses against host tissues resulting in GVHD.…”

Section: Resultssupporting

confidence: 63%

“…7 G-CSF also mobilizes tolerogenic CD14 ϩ cells expressing cell surface IL-10. 8,9 So far, G-CSF has been shown to selectively mobilize plasmacytoid dendritic cells (pDCs) that induce T cells to produce high levels of IL-4 and IL-10, but low levels of IFN-␥. 10,11 The in vivo effects of G-CSF on the large population (1.2% of peripheral blood mononuclear cells [PBMCs]) of proinflammatory 6-sulfoLacNAc expressing DCs, now called slanDCs, have not been studied so far.…”

Section: Introductionmentioning

confidence: 99%

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G-CSF mobilizes slanDCs (6-sulfo LacNAc+ dendritic cells) with a high proinflammatory capacity

Baumeister

Hölig²,

Bornhäuser³

et al. 2007

Blood

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Section: Resultssupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

G-CSF mobilizes slanDCs (6-sulfo LacNAc+ dendritic cells) with a high proinflammatory capacity

Baumeister

Hölig²,

Bornhäuser³

et al. 2007

Blood

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“…In our study, the production of IL-10 after application of G-CSF increased during the first 20 h, and then decline over a period of 60-80 h. Fraser et al [9] proved that the production of IL-10 in donor cells mainly came from monocytes after being stimulated with LPS.…”

Section: Cd3mentioning

confidence: 64%

“…Some retrospective studies have shown that after 2 years from a HSCT-PB, disease free survival (DFS) is better and the incidence of relapse is lower [2,[9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Granulocyte colony‐stimulating factor produces a decrease in IFNγ and increase in IL‐4 when administrated to healthy donors

Rodríguez‐Cortés

Vela‐Ojeda

López‐Santiago

et al. 2010

J of Clinical Apheresis

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Hematopoietic stem cells transplantation (HSCT) is the leading curative therapy for a variety of hematological and hereditary diseases; however, graft versus host disease (GVHD), an immunologic phenomenon that is favored by Th1 cytokines and cytotoxic cells from donors, is present frequently and is one of the most important causes of transplant related mortality. Peripheral blood HSCT is the preferred source of stem cells in almost 100% of the cases of autologous HSCT and in 70% of allogeneic transplants. The best mobilizing agent to get the stem cells out from the bone marrow is the Granulocyte-Colony Stimulating Factor (G-CSF). In this work, our main objective was to study a possible correlation between the graft cell dose and the patient's clinical outcome. We evaluated the immunologic changes produced by G-CSF in the lymphocyte and cytokine profiles in allogeneic HSC donors. HSC from twelve donors were mobilized with G-CSF at 16 microg/kg/day, for 5 days. Basal Peripheral Blood (BPB), Mobilized Peripheral Blood (MPB), and aphaeresis mononuclear cells (G-MNC) samples were taken from all donors. Using flow cytometry, we quantified CD19(+), CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), NK, NKT, DC1, and DC2 cells. Cytokines were determined by ELISA in culture supernatants. CD19(+) (p = 0.001), DC1 (p < 0.002) and DC2 (p < 0.001) cells were increased in MPB with respect to BPB. An increase in Th2 cytokines such as (IL-4) and a decrease in Th1 cytokines (IFNgamma, IL-2) were also found in MPB samples. In conclusion, Th1 and Th2 cytokines are relevant in predicting the clinical outcome after allogeneic peripheral blood HSCT.

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“…Topcuoglu et al 30 reported similar findings. Leblanc et al 26 found increased levels of factor VIII:C and thrombin generation in healthy donors after rhG-CSF administration. Söhngen et al 31 detected increased factor VIII and fibrinogen levels, while protein C and protein S activities were reduced.…”

Section: Platelets and Coagulationmentioning

confidence: 99%

Biologic and molecular effects of granulocyte colony-stimulating factor in healthy individuals: recent findings and current challenges

Anderlini

Champlin

2008

Blood

156

143

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Immature monocytes from G-CSF-mobilized peripheral blood stem cell collections carry surface-bound IL-10 and have the potential to modulate alloreactivity

Cited by 27 publications

References 32 publications

G-CSF mobilizes slanDCs (6-sulfo LacNAc+ dendritic cells) with a high proinflammatory capacity

G-CSF mobilizes slanDCs (6-sulfo LacNAc+ dendritic cells) with a high proinflammatory capacity

Granulocyte colony‐stimulating factor produces a decrease in IFNγ and increase in IL‐4 when administrated to healthy donors

Biologic and molecular effects of granulocyte colony-stimulating factor in healthy individuals: recent findings and current challenges

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