Surface patterning: Tool to modulate stem cell differentiation in an adipose system

Abstract

There are several issues that need to be better understood before breast tissueengineering becomes viable clinically. One of the key issues is the interaction between cells and the microtopography of the implant surface. The aim of this study was to evaluate the efficacy of D1 cells, multipotent mouse bone marrow stromal precursors, in differentiating to fat and to characterize their metabolic activity (lactic acid released and glucose consumed) and lipid production when cultured on patterned poly-L-lactide (PLLA) films. It was determined that, with appropriate stimulation, the D1 cells displayed morphological characteristics of adipocytes and produced lipid. The results show that the patterned surfaces did affect the rate of lipid production. Polynomial models were proposed to predict the metabolic activity of the cells over a period of time. '

Application of hybrid agarose‐aminosilane gels to the biofunctionalization of honeycomb‐ structured polycaprolactone scaffolds

“…[12] In parallel studies, agarose-APTS replicas could be used as platforms for cell growth on surfaces with controlled patterning and chemical functionalization. [13] …”

Phenomenon of “contact guidance“ on the surface with nano-micro-groove-like pattern and cell physiological effects

“…Currently, with the deepened research and the improvements made in fabricating patterns, the researches have begun to embody the following features: first, the combination of patterns and non-pattern factors. This mainly includes two sub-areas: a) the combination of pattern and bioactive molecules [54] and b). the combination of patterns and extra forces [22] .…”

“…Gomez et al [53] also reported that "contact guidance" can effectively induce the polarization and axon formation of embryonic hippocampal neurons. On the other hand, Chaubey et al [54] compared the influence of microgrooves made of different substrates on multipotent mouse bone marrow stromal cells (D1 cells). According to their research, the D1 cells cultured on PLLA substrates displayed an intermediate rate of lipid production through out the experiment, whereas at the early stage of the experiment, the lipid production of the D1 cells cultured on PS substrates was higher than that of the cells cultured on PLLA substrate, but the trend was reversed at the late stage.…”

Micro- and Nanotechnology in Tissue Engineering

“…An example is the work described by D. Zahor and colleagues [149] who have induced topography-guided alignment of mesenchymal stem cells by culturing them on micropatterned silicon surfaces. Another study evaluated the efficacy of a micropatterned surface in differentiating multipotent mouse bone marrow stromal precursors into fat tissue [26]. The results showed the positive influence of the patterns in lipid production.…”