Surface nanotopography guides kidney-derived stem cell differentiation into podocytes

MacGregor, Melanie; Hopp, Isabel; Bachhuka, Akash; Murray, Patricia; Vasilev, Krasimir

doi:10.1016/j.actbio.2017.02.036

Cited by 28 publications

(34 citation statements)

References 60 publications

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“…Nanosized topographical features have been reported in the natural extra cellular matrix and it is now believed that only nano textures of sizes similar to those of natural proteins affect their potential denaturation . Surfaces with tailored nanotopography are being investigated as novel cell culture substrate because they can be tuned to improve cell adhesion, modulate immune response and direct stem cells differentiation into targeted lineages . Additionally, careful surface nanoengineering can also help controlling bacterial adhesion and biofilm formation .…”

Section: Why Nanotopography?mentioning

confidence: 99%

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Questions and Answers on the Wettability of Nano‐Engineered Surfaces

MacGregor

Vasilev

2017

Adv Materials Inter

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Surface roughening has long been used to confer materials remarkable wetting properties, such as super hydrophobicity. When roughness belongs to the microscale, existing models can, to some extent, explain and predict real‐world wetting states. With the advent of nanotechnology, however, a multitude of nano‐engineered materials are being developed and unexpected wetting behaviors have been observed which cannot be described by conventional theories. While it is clear that advanced nanotextured materials are essential to a vast range of ground breaking technologies, the intricate mechanisms governing the wetting of such surfaces—at the limit of validity of continuum theories—also need to be clarified. This review aims at presenting what is known and what remains to be discovered about the wettability of nanoengineered materials. The advantages and uniqueness of nanostructured substrates is highlighted first, with a focus on practical applications benefitting from the distinctive wetting properties of nanorough substrates. Classic wetting theories and their limitations are briefly discussed, before describing with a top down vision, why the wetting of nanostructured substrates differ from that of microtextured surfaces. Recent experimental and theoretical studies which contributed to the progress in this field are considered, before outlining potential future areas of research.

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Section: Why Nanotopography?mentioning

confidence: 99%

“…Copyright 2014, National Institute for Materials Science. d) Surface density gradient of monodisperse gold nanoparticles, 16 and 68 nm in diameter, generated by dip coating of a plasma deposited nitrogen rich thin organic film …”

Section: Introductionmentioning

confidence: 99%

Questions and Answers on the Wettability of Nano‐Engineered Surfaces

MacGregor

Vasilev

2017

Adv Materials Inter

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“…15 Nanotopographic cues are well-known technology for controlling and manipulating cells into becoming possible therapeutic candidates for treatment of cardiac, renal, and vascular diseases. [16][17][18] Nanotopographic cues affect stem cell adhesion, migration, proliferation, differentiation, and apoptosis by adjusting their structures, surface stiffness, and porosity. 19 Tendons are aligned structures, and decreased tendon function caused by tendinopathy and tendon injury is associated with tendon misalignment.…”

Section: Introductionmentioning

confidence: 99%

Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions

Kim¹,

Tatman²,

Song³

et al. 2018

IJN

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BackgroundTendon-derived stem cells (TDSCs) are key factors associated with regeneration and healing in tendinopathy. The aim of this study was to investigate the effects of mechanical stiffness and topographic signals on the differentiation of TDSCs depending on age and pathological conditions.Materials and methodsWe compared TDSCs extracted from normal tendon tissues with TDSCs from tendinopathic Achilles tendon tissues of Sprague Dawley rats in vitro and TDSCs cultured on nanotopographic cues and substrate stiffness to determine how to control the TDSCs. The tendinopathy model was created using a chemical induction method, and the tendon injury model was created via an injury-and-overuse method. Norland Optical Adhesive 86 (NOA86) substrate with 2.48 GPa stiffness with and without 800 nm-wide nanogrooves and a polyurethane substrate with 800 nm-wide nanogrooves were used.ResultsTDSCs from 5-week-old normal tendon showed high expression of type III collagen on the flat NOA86 substrate. In the 15-week normal tendon model, expression of type III collagen was high in TDSCs cultured on the 800 nm NOA86 substrates. However, in the 15-week tendon injury model, expression of type III collagen was similar irrespective of nanotopographic cues or substrate stiffness. The expression of type I collagen was also independent of nanotopographic cues and substrate stiffness in the 15-week normal and tendon injury models. Gene expression of scleraxis was increased in TDSCs cultured on the flat NOA86 substrate in the 5-week normal tendon model (P=0.001). In the 15-week normal tendon model, scleraxis was highly expressed in TDSCs cultured on the 800 nm and flat NOA86 substrate (P=0.043). However, this gene expression was not significantly different between the substrates in the 5-week tendinopathy and 15-week tendon injury models.ConclusionDevelopment and maturation of tendon are enhanced when TDSCs from normal tendons were cultured on stiff surface, but not when the TDSCs came from pathologic models. Therapeutic applications of TDSCs need to be flexible based on tendon age and tendinopathy.

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“…We have demonstrated recently that tailored surface roughness, including roughness comparable with this inherent for the model samples used in this study, was capable of guiding the differentiation of mKSCs to podocytes. 23 This nding was not surprising since it is now well accepted that surface nanotopography has signicant inuence on the fate of various types of cells, including immune and stem cells.…”

Section: Proximal Tubule Cell Differentiationmentioning

confidence: 99%

“…19,20,23 To investigate if the propensity of cells to undergo podocyte differentiation varied between different substrates, we rst measured the extent of cell spreading, because podocyte-like cells tend to have a large cytoplasm : nuclear ratio. F-Actin staining was used to facilitate evaluation of cell spreading area (Fig.…”

Section: Podocyte Differentiationmentioning

confidence: 99%

Silver nanoparticle modified surfaces induce differentiation of mouse kidney-derived stem cells

et al. 2018

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In this paper, we interrogate the influence of silver nanoparticle (AgNPs)-based model surfaces on mouse kidney-derived stem cells (mKSCs) differentiation. The widespread use of silver in biomedical and consumer products requires understanding of this element's effect on kidney cells. Moreover, the potential for using stem cells in drug discovery require methods to direct their differentiation to specialized cells. Hence, we generated coated model substrates containing different concentrations of surface immobilized AgNPs, and used them to evaluate properties and functions of mKSCs. Initially, mKSCs exhibited reduced viability on higher silver containing surfaces. However, longer culture periods assisted mKSCs to recover. Greater degree of cell spreading and arborization led by AgNPs, suggest podocyte differentiation. Proximal tubule cell marker's expression revealed differentiation to the specific lineage. Although the exact mechanism underpinning these findings require significant future efforts, this study demonstrate silver's capacity to stimulate mKSC differentiation, which may provide opportunities for drug screenings.

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Surface nanotopography guides kidney-derived stem cell differentiation into podocytes

Cited by 28 publications

References 60 publications

Questions and Answers on the Wettability of Nano‐Engineered Surfaces

Questions and Answers on the Wettability of Nano‐Engineered Surfaces

Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions

Silver nanoparticle modified surfaces induce differentiation of mouse kidney-derived stem cells

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