Surface modified superparamagnetic nanoparticles: Interaction with fibroblasts in primary cell culture

González, Christian Chapa; Roacho-Pérez, Jorge A.; Perez, Carlos A.; Olivas–Armendáriz, I.; Jiménez-Vega, Florinda; Parga, Karen Yael Castrejón; García-Casillas, Perla E.

doi:10.1016/j.jallcom.2014.01.155

Cited by 9 publications

(9 citation statements)

References 17 publications

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“…Magnetite nanoparticles were synthetized by chemical co-precipitation based on the modified methodology of Chapa-González et al [20]. We used two precursor solutions: 100 mL of ferric chloride hexahydrate 0.01 M (FeCl 3 •6H 2 O, Thermo Fisher Scientific, Waltham, MA, USA) and 100 mL of ferrous sulfate heptahydrated 0.05 M (FeSO 4 •7H 2 O, Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Magnetite Nanoparticle Synthesismentioning

confidence: 99%

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Magnetite Nanoparticles Coated with PEG 3350-Tween 80: In Vitro Characterization Using Primary Cell Cultures

et al. 2020

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Some medical applications of magnetic nanoparticles require direct contact with healthy tissues and blood. If nanoparticles are not designed properly, they can cause several problems, such as cytotoxicity or hemolysis. A strategy for improvement the biological proprieties of magnetic nanoparticles is their functionalization with biocompatible polymers and nonionic surfactants. In this study we compared bare magnetite nanoparticles against magnetite nanoparticles coated with a combination of polyethylene glycol 3350 (PEG 3350) and polysorbate 80 (Tween 80). Physical characteristics of nanoparticles were evaluated. A primary culture of sheep adipose mesenchymal stem cells was developed to measure nanoparticle cytotoxicity. A sample of erythrocytes from a healthy donor was used for the hemolysis assay. Results showed the successful obtention of magnetite nanoparticles coated with PEG 3350-Tween 80, with a spherical shape, average size of 119.2 nm and a zeta potential of +5.61 mV. Interaction with mesenchymal stem cells showed a non-cytotoxic propriety at doses lower than 1000 µg/mL. Interaction with erythrocytes showed a non-hemolytic propriety at doses lower than 100 µg/mL. In vitro information obtained from this work concludes that the use of magnetite nanoparticles coated with PEG 3350-Tween 80 is safe for a biological system at low doses.

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Section: Magnetite Nanoparticle Synthesismentioning

confidence: 99%

“…Magnetite (Fe 3 O 4 ) is an iron mineral that is composed of a combination of oxide Fe 2+ and Fe 3+ . Magnetite nanoparticle synthesis was performed by the nanoprecipitation method, where the chemical reaction that takes place for the formation of magnetite is represented by the Equation (1) [20]:…”

Section: Magnetite Nanoparticle Synthesismentioning

confidence: 99%

Magnetite Nanoparticles Coated with PEG 3350-Tween 80: In Vitro Characterization Using Primary Cell Cultures

et al. 2020

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“…These results were in agreement with previous zeta potential determinations on magnetite. 14 In alkaline media, magnetite is negatively charged as a result of rising accumulation of OH on the surface. When CS solution has been poured to the nanoparticle suspension, the chitosan molecules have immobilized on the surface of the nanoparticles causing the surface potential to increase (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Preparation of magnetite-(Fe 3 O 4 ) and chitosan-coated magnetite nanocomposites Nanocomposites were synthesized as previously described. 14,19 The synthesis was carried out by means of the co-precipitation reaction, in which a stoichiometric ratio of 2 to 1 with respect to the Fe 3+ and Fe 2+ ions is preserved. First, 100 mL of 0.1 M ferric chloride (FeCl 3 ) and 0.5 M ferrous sulfate (FeSO 4 ) were prepared separately and both solutions were mixed with vigorous stirring for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical properties of chitosan–magnetite nanocomposites obtained with different pH

González

Arriaga

García-Casillas

2021

Polymers and Polymer Composites

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The physicochemical properties of the nanoparticle surface determine the performance of nanocomposites in biomedical applications such as their biodistribution and pharmacokinetics. The physicochemical properties of chitosan, such as apparent charge density and solubility, are pH dependent. Similarly, Fe3O4 nanoparticles are susceptible to variations in their physicochemical properties due to changes in pH. In this work, we evaluated the physicochemical properties of chitosan–magnetite nanocomposites that were suspended at pH 7.0, 9.0, and 11.0 to determinate the effect on particle size, zeta potential, and mass percentage of the polymeric coating, in addition to the crystalline phase and magnetic properties of magnetite phase. X-ray diffraction results exposed that the present phase was magnetite with no other phases present and that the crystallite size was between 10.8 and 14.1 nm. Fourier transform infrared verified the chitosan functional groups in treated samples while the percentage of mass determined by TGA found to be nearly 9%. Scanning electron microscopy micrographs corroborated the spherical shape of the bare and chitosan-coated nanoparticles. Dynamic light scattering results showed that chitosan coating modifies the zeta potential, going from a potential of −11.8 mV for bare particles to −3.0 mV (pH 11). Besides, vibrating sample magnetometer measurements showed that coercivity remained very low, which is desirable in biomedical applications.

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“…[3][4][5][6][7] In the same way, some researchers have focused their attention on surface modification to improve the prop erties of the nanoparticles. [8][9][10][11][12][13][14][15][16][17] One of the strategies that researchers have followed to modify the surface of nanoparticles and provide functionality has been to use coupling agents. Aminosilane is a coupling agent present in the nanomaterials used for biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Effect of Aminosilane Nanoparticle Coating on Structural and Magnetic Properties and Cell Viability in Human Cancer Cell Lines

García

Loredo

Galindo

et al. 2022

Part & Part Syst Charact

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Magnetic nanoparticle interfaces have aroused great scientific research interest in the biomedical area since the interaction of cells or biomolecules with nanoparticles is determined by the surface properties. Currently, in medical applications, there is a need to study cell interaction and growth, along with changes in structural or magnetic properties, attributed to nanoparticle coatings. In this study the coercive field changes in NixFe3‐xO4 nanoparticles (x = 0.0, 0.2, 0.4, 0.6, 0.8, and 1.0) driven by partial or total substitution of Fe2+ content by Ni2+, and by aminosilane coating are evaluated. The nanoparticles are synthesized by the coprecipitation method. The inverse spinel structure is confirmed by X‐ray diffraction results and Raman spectra. The aminosilane coating is confirmed by energy‐dispersive X‐ray spectroscopy and Fourier transform infrared spectroscopy. Dynamic light scattering confirms a mean hydrodynamic size of 10 nm. Scanning electron microscopy micrographs of the uncoated and aminosilane‐coated samples show that the particles have a hemispherical shape. The coating increases the coercive field. In addition, uncoated Ni0.2Fe2.8O4 has the highest viability in both MCF7 and HeLa cell lines, and aminosilane coating decreases cell viability. This study contributes to future applications of nanomedicine, such as hyperthermia and drug delivery.

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Surface modified superparamagnetic nanoparticles: Interaction with fibroblasts in primary cell culture

Cited by 9 publications

References 17 publications

Magnetite Nanoparticles Coated with PEG 3350-Tween 80: In Vitro Characterization Using Primary Cell Cultures

Magnetite Nanoparticles Coated with PEG 3350-Tween 80: In Vitro Characterization Using Primary Cell Cultures

Physicochemical properties of chitosan–magnetite nanocomposites obtained with different pH

Effect of Aminosilane Nanoparticle Coating on Structural and Magnetic Properties and Cell Viability in Human Cancer Cell Lines

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