Surface modification to enhance cell migration on biomaterials and its combination with 3D structural design of occluders to improve interventional treatment of heart diseases

Shen, Yang; Zhang, Wanqian; Xie, Yumei; Li, Anning; Wang, Xiuli; Chen, Xianmiao; Liu, Qingsong; Wang, Qunsong; Gui, Zhilun; Liu, Qiong; Liu, Jianxiong; Zhang, Deyuan; Zhang, Zhiwei; Ding, Jiandong

doi:10.1016/j.biomaterials.2021.121208

Cited by 47 publications

(31 citation statements)

References 82 publications

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“…As is known, many physiological activities of the human body such as cell signaling, regulation of enzyme activity, blood clotting, hormonal activities and so on are related to calcium ions. 38,39 The normal extracellular and intracellular concentrations of calcium ions are about 2 mM and 100 nM, respectively. 40 The imbalance of calcium concentration may cause damage to cells.…”

Section: Resultsmentioning

confidence: 99%

A coordination strategy to achieve instant dissolution of a biomedical polymer in water via manual shaking

et al. 2022

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Section: Resultsmentioning

confidence: 99%

A coordination strategy to achieve instant dissolution of a biomedical polymer in water via manual shaking

et al. 2022

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“…Shen et al . indicated that Ni 2+ toxicity and processes such as cell adhesion and migration may be connected [ 22 ]. Therefore, the CSF1 gene/protein was not selected as a biomarker for assessing Ni 2+ cytotoxicity.…”

Section: Resultsmentioning

confidence: 99%

Screening and validation of nickel ion cytotoxicity biomarkers based on transcriptomic and proteomic technology

Zhang

Huang

Lü

2022

Regenerative Biomaterials

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The aim of this study was to screen cytotoxicity biomarkers of nickel ions (Ni2+) using transcriptomic and proteomic approaches combined with molecular biology validation. First, the MTT method was used to evaluate cytotoxicity in L929 cells treated with Ni2+ at different concentrations. Ni2+ at both 100 μM and 200 μM affected cell proliferation. Then, transcriptomic and proteomic technology was used to study the effects of Ni2+ on the expression of genes/proteins in cells. It was found that 1490, 789, 652 and 729 genes (12, 24, 48 and 72 h, respectively) and 177, 2191 and 2095 proteins (12, 24 and 48 h, respectively) were differentially expressed after treatment with 100 μM Ni2+. In total, 1403, 963, 916 and 1230 genes (12, 24, 48 and 72 h, respectively) and 83, 1681 and 2398 proteins (12, 24 and 48 h, respectively) were differentially expressed after treatment with 200 μM Ni2+. Then, 4 target gene/protein biomarkers were filtered by combined screening using gene/proteomic experimental data and biological pathway analyses. Further expression level validation of all these target biomarkers and functional validation of selected gene/protein biomarkers were carried out, and a final gene/protein biomarker (UQCRB) was identified.

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“…Compared to some small-molecular organic agents and some metals, polymers are hard to be “visualizable” in the interventional treatment. Different from metal implants, additional radiopaque markers such as platinum have to be added in biodegradable polymers so as to enhance X-ray imaging contrast and therefore facilitate the operation during implantation. , However, these nonbiodegradable metal radiopaque markers will remain in the human body permanently. Besides, people can only judge the position of implants via a few dots other than directly viewing whole implants under X-ray, which is not convenient for an operator.…”

Section: Introductionmentioning

confidence: 99%

A Facile Composite Strategy to Prepare a Biodegradable Polymer Based Radiopaque Raw Material for “Visualizable” Biomedical Implants

Wang

Chen

et al. 2022

ACS Appl. Mater. Interfaces

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Enabling a biodegradable polymer radiopaque under X-ray is much desired for many medical devices. Physical blending of a present biodegradable polymer and a commercialized medical contrast agent is convenient yet lacks comprehensive fundamental research. Herein, we prepared a biodegradable polymer-based radiopaque raw material by blending poly(L-lactic acid) (PLLA or simply PLA) and iohexol (IHX), where PLA constituted the continuous phase and IHX particles served as the dispersed phase. The strong X-ray adsorption of IHX enabled the composite radiopaque; the hydrolysis of the polyester and the water solubility of the contrast agent enabled the composite biodegradable in an aqueous medium. The idea was confirmed by in vitro characterizations of the resultant composite, in vivo subcutaneous implantation in rats up to 6 months, and the clear visualization of a part of a biodegradable occluder in a Bama piglet under X-ray. We also found that the crystallization of PLA was significantly enhanced in the presence of the solid particles, which should be taken into consideration in the design of an appropriate biomaterial composite because crystallization degree influences the biodegradation rate and mechanical property of a material to a large extent. We further tried to introduce a small amount of poly(vinylpyrrolidone) into the blend of PLA and IHX. Compared to the bicomponent composite, the tricomponent one exhibited decreased modulus and increased elongation at break and tensile strength. This paves more ways for researchers to select appropriate raw materials according to the regenerated tissue and the application site.

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Surface modification to enhance cell migration on biomaterials and its combination with 3D structural design of occluders to improve interventional treatment of heart diseases

Cited by 47 publications

References 82 publications

A coordination strategy to achieve instant dissolution of a biomedical polymer in water via manual shaking

A coordination strategy to achieve instant dissolution of a biomedical polymer in water via manual shaking

Screening and validation of nickel ion cytotoxicity biomarkers based on transcriptomic and proteomic technology

A Facile Composite Strategy to Prepare a Biodegradable Polymer Based Radiopaque Raw Material for “Visualizable” Biomedical Implants

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