Surface Modification to Control Protein/Surface Interactions

Lin, Yuan; Yu, Qian; Li, Dan; Chen, Hong

doi:10.1002/mabi.201000464

Cited by 75 publications

(48 citation statements)

References 81 publications

(162 reference statements)

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“…Although technically simple, the “grafting to” strategy suffers from several limitations. For example, steric repulsion between growing grafted polymer chains may hamper the formation of polymer brushes having high MW and graft density 87–89. By contrast, in the “grafting from” strategy the density and MW of grafted chains is not sterically limited unless a very high graft molecular weight is approached.…”

Section: Surface Modification Using Pvpmentioning

confidence: 99%

Poly(N‐vinylpyrrolidone)‐Modified Surfaces for Biomedical Applications

Liu

et al. 2012

Macromolecular Bioscience

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181

121

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Poly(N-vinylpyrrolidone) (PVP), an important water soluble synthetic polymer, has many desirable properties including low toxicity, chemical stability, and good biocompatibility. Since PVP is hemocompatible and physiologically inactive, it has been used as a blood plasma substitute. Surface modification with PVP has been investigated extensively over the past few years as a means of preventing nonspecific protein adsorption. PVP may therefore be seen as a promising antifouling surface modifier comparable to poly(ethylene glycol) (PEG). In this review, various approaches for the design and preparation of PVP-modified surfaces are summarized and potential biomedical applications of these PVP-modified materials are indicated. Finally, some perspectives on future research on PVP for surface modification are discussed.

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Section: Surface Modification Using Pvpmentioning

confidence: 99%

Poly(N‐vinylpyrrolidone)‐Modified Surfaces for Biomedical Applications

Liu

et al. 2012

Macromolecular Bioscience

Self Cite

181

121

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“…The adsorbed proteins play an important role in material‐associated clotting or platelet adhesion and activation, and then lead to thrombus formation, thus protein adsorption on material surface is believed to be crucial for blood compatibility . As widely known, the surface hydrophilicity/hydrophobicity has great influence on protein adsorption . Hydrophilic surface can bind water molecules to form a hydration layer, which can passivate the material surfaces to repel protein adsorption .…”

Section: Resultsmentioning

confidence: 99%

Super-Anticoagulant Heparin-Mimicking Hydrogel Thin Film Attached Substrate Surfaces to Improve Hemocompatibility

Cui

Jiang

et al. 2016

Macromol. Biosci.

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In this study, heparin-mimicking hydrogel thin films are covalently attached onto poly(ether sulfone) membrane surfaces to improve anticoagulant property. The hydrogel films display honeycomb-like porous structure with well controlled thickness and show long-term stability. After immobilizing the hydrogel films, the membranes show excellent anticoagulant property confirmed by the activated partial thromboplastin time values exceeding 600 s. Meanwhile, the thrombin time values increase from 20 to 61 s as the sodium allysulfonate proportions increase from 0 to 80 mol%. In vitro investigations of protein adsorption and blood-related complement activation also confirm that the membranes exhibit super-anticoagulant property. Furthermore, gentamycin sulfate is loaded into the hydrogel films, and the released drug shows significant inhibition toward E. coli bacteria. It is believed that the surface attached heparin-mimicking hydrogel thin films may show high potential for the applications in various biological fields, such as blood contacting materials and drug loading materials.

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“…This allowed pioneering studies on the effect of substrate stiffness on mesenchymal stem cell differentiation . Usually, protein adsorption and cell adhesion do take place rather slowly on PDMS . Thus, short‐term cell specificity and geometrical control of cell shape can be induced by micro‐contact printing (μCP) of proteins or peptides directly onto PDMS .…”

Section: Introductionmentioning

confidence: 99%

“…[12,13] Usually, protein adsorption and cell adhesion do take place rather slowly on PDMS. [9,14,15] Thus, short-term cell specificity and geometrical control of cell shape can be induced by micro-contact printing (mCP) of proteins or peptides directly onto PDMS. [1,[16][17][18] Especially under standard cell culture conditions, adsorption of proteins onto the PDMS, either from the cell culture medium or secreted by the cells themselves, significantly overlays the initially generated protein/ peptide pattern.…”

Section: Introductionmentioning

confidence: 99%

Stable Biochemically Micro-patterned Hydrogel Layers Control Specific Cell Adhesion and Allow Long Term Cyclic Tensile Strain Experiments

et al. 2014

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Poly(dimethylsiloxane) can be covalently coated with ultrathin NCO-sP(EO-stat-PO) hydrogel layers which permit covalent binding of cell adhesive moieties, while minimizing unspecific cell adhesion on non-functionalized areas. We applied long term uniaxial cyclic tensile strain (CTS) and revealed (a) the preservation of protein and cell-repellent properties of the NCO-sP(EO-stat-PO) coating and (b) the stability and bioactivity of a covalently bound fibronectin (FN) line pattern. We studied the adhesion of human dermal fibroblast (HDFs) on non-modified NCO-sP(EO-stat-PO) coatings and on the FN. HDFs adhered to FN and oriented their cell bodies and actin fibers along the FN lines independently of the direction of CTS. This mechanical long term stability of the bioactive, patterned surface allows unraveling biomechanical stimuli for cellular signaling and behavior to understand physiological and pathological cell phenomenon. Additionally, it allows for the application in wound healing assays, tissue engineering, and implant development demanding spatial control over specific cell adhesion.

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Surface Modification to Control Protein/Surface Interactions

Cited by 75 publications

References 81 publications

Poly(N‐vinylpyrrolidone)‐Modified Surfaces for Biomedical Applications

Poly(N‐vinylpyrrolidone)‐Modified Surfaces for Biomedical Applications

Super-Anticoagulant Heparin-Mimicking Hydrogel Thin Film Attached Substrate Surfaces to Improve Hemocompatibility

Stable Biochemically Micro-patterned Hydrogel Layers Control Specific Cell Adhesion and Allow Long Term Cyclic Tensile Strain Experiments

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