Surface modification of titanium implant for repairing/improving microenvironment of bone injury and promoting osseointegration

Ding, Yao; Tao, Bailong; Ma, Ruichen; Zhao, Xin; Liu, Peng; Cai, Kun

doi:10.1016/j.jmst.2022.09.044

Cited by 29 publications

(17 citation statements)

References 26 publications

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“…51 Several workers showed that Ti could be a material of importance in bone injury and implant fixation also. 52,53 In the present study, the prepared scaffolds had a porosity range of 19−27% and the porosity is higher for the Ti-doped samples (Table 1). This may be due to the shortening of the axis by substitution with titanium.…”

Section: Discussionmentioning

confidence: 51%

“…A few reports on titanium oxide and Ti as a trace element and their role in osteogenesis is available although a significant number of studies have been done with titanium, taking it as a surface modification agent or as an alloy in metal prosthesis . Several workers showed that Ti could be a material of importance in bone injury and implant fixation also. , …”

Section: Discussionmentioning

confidence: 99%

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In Vitro and In Vivo Bone Regeneration Assessment of Titanium-Doped Waste Eggshell-Derived Hydroxyapatite in the Animal Model

Acharjee

Mandal

Samanta³

et al. 2023

ACS Biomater. Sci. Eng.

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In this work, a titanium-doped hydroxyapatite (HAp) scaffold was produced from two different sources (natural eggshell and laboratory-grade reagents) to compare the efficacy of natural and synthetic resources of HAp materials on new bone regeneration. This comparative study also reports the effect of Ti doping on the physical, mechanical, and in vitro as well as in vivo biological properties of the HAp scaffold. Pellets were prepared in the conventional powder metallurgy route, compacted, and sintered at 900 °C, showing sufficient porosity for bony ingrowth. The physical-mechanical characterizations were performed by density, porosity evaluation, XRD, FTIR, SEM analysis, and hardness measurement. In vitro interactions were evaluated by bactericidal assay, hemolysis, MTT assay, and interaction with simulated body fluid. All categories of pellets showed absolute nonhemolytic and nontoxic character. Furthermore, significant apatite formation was observed on the Tidoped HAp samples in the simulated body fluid immersion study. The developed porous pellets were implanted to assess the bone defect healing in the femoral condyle of healthy rabbits. A 2 month study after implantation showed no marked inflammatory reaction for any samples. Radiological analysis, histological analysis, SEM analysis, and oxytetracycline labeling studies depicted better invasion of mature osseous tissue in the pores of doped eggshell-derived HAp scaffolds as compared to the undoped HAp, and laboratory-made samples. Quantification using oxytetracycline labeling depicted 59.31 ± 1.89% new bone formation for Ti-doped eggshell HAp as compared to Ti-doped pure HAp (54.41 ± 1.93) and other undoped samples. Histological studies showed the presence of abundant osteoblastic and osteoclastic cells in Ti-doped eggshell HAp in contrast to other samples. Radiological and SEM data also showed similar results. The results indicated that Ti-doped biosourced HAp samples have good biocompatibility, new bone-forming ability, and could be used as a bone grafting material in orthopedic surgery.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Bone Regeneration Assessment of Titanium-Doped Waste Eggshell-Derived Hydroxyapatite in the Animal Model

Acharjee

Mandal

Samanta³

et al. 2023

ACS Biomater. Sci. Eng.

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“…These pro-inflammatory cytokines augment the activity of osteoclasts and decrease the recruitment or migration of MSCs. This results in bone disconnection and poor osseointegration [ 68 – 72 ]. In this study, Ti-PDA@SNP-OGP significantly down-regulated the mRNA expression levels of M1 markers CD86, iNOS, and CD11C and up-regulated the mRNA expression levels of M2 markers CD206, Arg-1, and CD163.…”

Section: Discussionmentioning

confidence: 99%

Elimination of methicillin-resistant Staphylococcus aureus biofilms on titanium implants via photothermally-triggered nitric oxide and immunotherapy for enhanced osseointegration

Lin

et al. 2023

Military Med Res

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Background Treatment of methicillin-resistant Staphylococcus aureus (MRSA) biofilm infections in implant placement surgery is limited by the lack of antimicrobial activity of titanium (Ti) implants. There is a need to explore more effective approaches for the treatment of MRSA biofilm infections. Methods Herein, an interfacial functionalization strategy is proposed by the integration of mesoporous polydopamine nanoparticles (PDA), nitric oxide (NO) release donor sodium nitroprusside (SNP) and osteogenic growth peptide (OGP) onto Ti implants, denoted as Ti-PDA@SNP-OGP. The physical and chemical properties of Ti-PDA@SNP-OGP were assessed by scanning electron microscopy, X-ray photoelectron spectroscope, water contact angle, photothermal property and NO release behavior. The synergistic antibacterial effect and elimination of the MRSA biofilms were evaluated by 2′,7′-dichlorofluorescein diacetate probe, 1-N-phenylnaphthylamine assay, adenosine triphosphate intensity, o-nitrophenyl-β-d-galactopyranoside hydrolysis activity, bicinchoninic acid leakage. Fluorescence staining, assays for alkaline phosphatase activity, collagen secretion and extracellular matrix mineralization, quantitative real‑time reverse transcription‑polymerase chain reaction, and enzyme-linked immunosorbent assay (ELISA) were used to evaluate the inflammatory response and osteogenic ability in bone marrow stromal cells (MSCs), RAW264.7 cells and their co-culture system. Giemsa staining, ELISA, micro-CT, hematoxylin and eosin, Masson’s trichrome and immunohistochemistry staining were used to evaluate the eradication of MRSA biofilms, inhibition of inflammatory response, and promotion of osseointegration of Ti-PDA@SNP-OGP in vivo. Results Ti-PDA@SNP-OGP displayed a synergistic photothermal and NO-dependent antibacterial effect against MRSA following near-infrared light irradiation, and effectively eliminated the formed MRSA biofilms by inducing reactive oxygen species (ROS)-mediated oxidative stress, destroying bacterial membrane integrity and causing leakage of intracellular components (P < 0.01). In vitro experiments revealed that Ti-PDA@SNP-OGP not only facilitated osteogenic differentiation of MSCs, but also promoted the polarization of pro-inflammatory M1 macrophages to the anti-inflammatory M2-phenotype (P < 0.05 or P < 0.01). The favorable osteo-immune microenvironment further facilitated osteogenesis of MSCs and the anti-inflammation of RAW264.7 cells via multiple paracrine signaling pathways (P < 0.01). In vivo evaluation confirmed the aforementioned results and revealed that Ti-PDA@SNP-OGP induced ameliorative osseointegration in an MRSA-infected femoral defect implantation model (P < 0.01). Conclusions These findings suggest that Ti-PDA@SNP-OGP is a promising multi-functional material for the high-efficient treatment of MRSA infections in implant replacement surgeries.

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“…Ding and colleagues approached inflammation from the perspective of directly combating oxidative stress. 239 They fabricated a coating on the titanium implant surface composed of manganese dioxide (MnO 2 ) and Sr 2+ (Fig. 7a).…”

Section: Metal Ion-based Biomaterials For Bone Regenerationmentioning

confidence: 99%

Metal ions: the unfading stars of bone regeneration—from bone metabolism regulation to biomaterial applications

Luo,

Liu,

Zhang

et al. 2023

Biomater. Sci.

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Surface modification of titanium implant for repairing/improving microenvironment of bone injury and promoting osseointegration

Cited by 29 publications

References 26 publications

In Vitro and In Vivo Bone Regeneration Assessment of Titanium-Doped Waste Eggshell-Derived Hydroxyapatite in the Animal Model

In Vitro and In Vivo Bone Regeneration Assessment of Titanium-Doped Waste Eggshell-Derived Hydroxyapatite in the Animal Model

Elimination of methicillin-resistant Staphylococcus aureus biofilms on titanium implants via photothermally-triggered nitric oxide and immunotherapy for enhanced osseointegration

Metal ions: the unfading stars of bone regeneration—from bone metabolism regulation to biomaterial applications

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