Surface modification of TiO2 nanotubes with osteogenic growth peptide to enhance osteoblast differentiation

Lai, Min; Jin, Ziyang; Su, Zhiguo

doi:10.1016/j.msec.2016.12.083

Cited by 51 publications

(29 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[11][12][13] But these elements or particles are difficult to directly incorporate into the stainless steel. Therefore, we set our sights on a commonly used metallurgy element -copper (Cu).…”

Section: Introductionmentioning

confidence: 99%

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Wang¹,

Li²,

Ren³

et al. 2017

IJN

View full text Add to dashboard Cite

Treatment for fractures requires internal fixation devices, which are mainly produced from stainless steel or titanium alloy without biological functions. Therefore, we developed a novel nano-copper-bearing stainless steel with nano-sized copper-precipitation (317L-Cu SS). Based on previous studies, this work explores the effect of 317L-Cu SS on fracture healing; that is, proliferation, osteogenic differentiation, osteogenesis-related gene expression, and lysyl oxidase activity of human bone mesenchymal stem cells were detected in vitro. Sprague–Dawley rats were used to build an animal fracture model, and fracture healing and callus evolution were investigated by radiology (X-ray and micro-CT), histology (H&E, Masson, and safranin O/fast green staining), and histomorphometry. Further, the Cu 2+ content and Runx2 level in the callus were determined, and local mechanical test of the fracture was performed to assess the healing quality. Our results revealed that 317L-Cu SS did not affect the proliferation of human bone mesenchymal stem cells, but promoted osteogenic differentiation and the expression of osteogenesis-related genes. In addition, 317L-Cu SS upregulated the lysyl oxidase activity. The X-ray and micro-CT results showed that the callus evolution efficiency and fracture healing speed were superior for 317L-Cu SS. Histological staining displayed large amounts of fibrous tissues at 3 weeks, and cartilage and new bone at 6 weeks. Further, histomorphometric analysis indicated that the callus possessed higher osteogenic efficiency at 6 weeks, and a high Cu 2+ content and increased Runx2 expression were observed in the callus for 317L-Cu SS. Besides, the mechanical strength of the fracture site was much better than that of the control group. Overall, we conclude that 317L-Cu SS possesses the ability to increase Cu 2+ content and promote osteogenesis in the callus, which could accelerate the callus evolution process and bone formation to provide faster and better fracture healing.

show abstract

Section: Introductionmentioning

confidence: 99%

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Wang¹,

Li²,

Ren³

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…13 According to previous reports, OGP has shown the ability to regulate proliferation, differentiation, and ECM mineralization for osteoblast cell lineages. [30][31][32][33][34][35] In this study, cells adhering onto both Ti-OGP and Ti-OGP-CIP substrates displayed well-spreading morphologies [ Fig. 6(A)].…”

Section: Discussionmentioning

confidence: 76%

“…Therefore, except for outstanding antibacterial properties, an ideal orthopedic implant is also required to have strong osseointegration, which encourage new bone deposition on its surface to speed up the stabilization process and to provide a seamless bone–implant interface . According to previous reports, OGP has shown the ability to regulate proliferation, differentiation, and ECM mineralization for osteoblast cell lineages . In this study, cells adhering onto both Ti–OGP and Ti–OGP–CIP substrates displayed well‐spreading morphologies [Fig.…”

Section: Discussionmentioning

confidence: 77%

“…Independently, growth factors play important roles in tissue repair and regeneration . Among the growth factors related to bone repair/regeneration, a naturally occurring 14‐mer peptide named osteogenic growth peptide (OGP) has been widely applied to improving bone regeneration . Nicole M. Moore et al reported an immobilized osteogenic growth peptide concentration gradient substrate technology and found that immobilized OGP increased MC3T3‐E1 osteoblast cell proliferation from 0 to 3 days at all densities.…”

Section: Introductionmentioning

confidence: 99%

“…28 Among the growth factors related to bone repair/regeneration, a naturally occurring 14-mer peptide named osteogenic growth peptide (OGP) has been widely applied to improving bone regeneration. [30][31][32][33][34][35] Nicole M. Moore et al 32 reported an immobilized osteogenic growth peptide concentration gradient substrate technology and found that immobilized OGP increased MC3T3-E1 osteoblast cell proliferation from 0 to 3 days at all densities. Besides, Policastro et al 33 proved that OGP tethered PEUs porous scaffolds revealed significant tissue-scaffold integration and the promotion of both osteogenesis and angiogenesis in vivo.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface

Liu

Yang

Tao

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

The inherent bioinertness and potential bacterial infection risk are the two leading causes for Ti implant failure. To improve osseointegration and antibiosis, in this work, a novel antimicrobial osteogenic growth peptide was first synthesized by conjugating osteogenic growth peptide (OGP) and ciprofloxacin (CIP). Then, the synthetic antimicrobial peptide was immobilized onto Ti implant surface for chemoselective binding via the amide reaction. Thereafter, the capabilities of modified Ti implant on osseointegration and antibiosis were measured with cell experiments and antimicrobial activity in vitro. The results showed that antimicrobial osteogenic growth peptide (OGP-CIP) was successfully prepared and grafted onto Ti implant surface. Moreover, the antimicrobial peptide-modified Ti implants could promote osteoblasts spreading and osteodifferentiation compared with unmodified Ti substrates. Meanwhile, in vitro bacteria studies (Staphylococcus aureus and Escherichia coli) proved that the antibacterial property of antimicrobial peptide functionalized Ti implant was improved obviously. The method used in this work is a feasible and promising strategy to win the race against invading bacteria and accelerate bone integration in orthopedic implantation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3021-3033, 2018.

show abstract

Characterization of aTiO₂ surfaces functionalized with CAP‐p15 peptide

Ureiro‐Cueto,

Rodil,

Santana‐Vázquez

et al. 2024

J Biomedical Materials Res

View full text Add to dashboard Cite

Functionalization of Titanium implants using adequate organic molecules is a proposed method to accelerate the osteointegration process, which relates to topographical, chemical, mechanical, and physical features. This study aimed to assess the potential of a peptide derived from cementum attachment protein (CAP‐p15) adsorbed onto aTiO2 surfaces to promote the deposition of calcium phosphate (CaP) minerals and its impact on the adhesion and viability of human periodontal ligament cells (hPDLCs). aTiO2 surfaces were synthesized by magnetron sputtering technique. The CAP‐p15 peptide was physically attached to aTiO2 surfaces and characterized by atomic force microscopy, fluorescence microscopy, and water contact angle measurement. We performed in vitro calcium phosphate nucleation assays using an artificial saliva solution (pH 7.4) to simulate the oral environment. morphological and chemical characterization of the deposits were evaluated by scanning electronic microscopy (SEM) and spectroscopy molecular techniques (Raman Spectroscopy, ATR‐FTIR). The aTiO2 surfaces biofunctionalized with CAP‐p15 were also analyzed for hPDLCs attachment, proliferation, and in vitro scratch‐healing assay. The results let us see that the homogeneous amorphous titanium oxide coating was 70 nanometers thick. The CAP‐p15 (1 μg/mL) displayed the ability to adsorb onto the aTiO2 surface, increasing the roughness and maintaining the hydrophilicity of the aTiO2 surfaces. The physical adsorption of CAP‐p15 onto the aTiO2 surfaces promoted the precipitation of a uniform layer of crystals with a flake‐like morphology and a Ca/P ratio of 1.79. According to spectroscopy molecular analysis, these crystalline deposits correspond to carbonated hydroxyapatite. Regarding cell behavior, the biofunctionalized aTiO2 surfaces improved the adhesion of hPDLCs after 24 h of cell culture, achieving 3.4‐fold when compared to pristine surfaces. Moreover, there was an increase in cell proliferation and cell migration processes. Physical adsorption of CAP‐p15 onto aTiO2 surfaces enhanced the formation of carbonate hydroxyapatite crystals and promoted the proliferation and migration of human periodontal ligament‐derived cells in in vitro studies. This experimental model using the novel bioactive peptide CAP‐p15 could be used as an alternative to increasing the osseointegration process of implants.

show abstract

Surface modification of TiO2 nanotubes with osteogenic growth peptide to enhance osteoblast differentiation

Cited by 51 publications

References 42 publications

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface

Characterization of aTiO₂ surfaces functionalized with CAP‐p15 peptide

Contact Info

Product

Resources

About

Surface modification of TiO2 nanotubes with osteogenic growth peptide to enhance osteoblast differentiation

Cited by 51 publications

References 42 publications

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface

Characterization of aTiO2 surfaces functionalized with CAP‐p15 peptide

Contact Info

Product

Resources

About

Characterization of aTiO₂ surfaces functionalized with CAP‐p15 peptide