Surface modification of polycaprolactone substrates using collagen-conjugated poly(methacrylic acid) brushes for the regulation of cell proliferation and endothelialisation

Abstract

The incorporation and presentation of cell recognition ligands on the surfaces of biodegradable bloodvessel implants to promote endothelialisation is considered to be a promising approach to prevent platelet aggregation and hence thrombogenesis. In this study, cell-adhesive collagen was covalently immobilised onto polycaprolactone (PCL) substrates via surface-initiated atom transfer radical polymerization (ATRP) to improve cell-material interactions. Functional polymer brushes of poly(methacrylic acid) (P(MAA)) containing dense and reactive carboxyl groups (-COOH) were formed on the PCL substrates in a controllable manner. The amount of collagen, which was conjugated to the pendant carboxyl groups via carbodiimide chemistry, increased with the concentration of -COOH groups on the grafted P(MAA) brushes. The affinity and growth of endothelial cells (ECs) were found to be significantly improved on the collagen-immobilised PCL substrates, and this improvement is positively correlated with the amount of covalently conjugated collagen. Thus, surfaceinitiated ATRP provides an alternative methodology for the surface functionalisation of biodegradable polyester scaffolds to enable the formation of a confluent layer of ECs. An optimally endothelialised material surface will play a major role in the minimisation of thrombogenicity and inflammation, and hence can be potentially used for vascular graft applications.

Dopaminergic Enhancement of Cellular Adhesion in Bone Marrow Derived Mesenchymal Stem Cells (MSCs)

“…Among the numerous approaches to improve cell seeding on the biomaterial surface, the most common techniques involve the use of a biomolecular coating such as collagen, chondroitin sulfate, sulfated hyaluronan, diamines, heparin, and RGD motif [28]. However, these coating methods experience limitations in regard to practical applications due to lack of stability and the requirement for complicated or expensive procedures [29-31]. Thus, the HGCS-PDA material with DA incorporated can be a good alternative candidate for these conventional methods.…”

Synthesis and Biomedical Applications of Poly((meth)acrylic acid) Brushes

“…Compared with conventional surfaces, a higher degree of biomolecule functionalization is achieved with P(M)AA brushes due to their 3D architecture and abundant carboxyl groups. Bovine serum albumin (BSA), 37 39,45,61,72,81,85,128,129 Fundamental research devoted to the NHS/EDC process has revealed unique aspects of this process for 3D P(M)AA brushes. A comparative study revealed that, while the major species obtained after activation was NHS ester for PAA brushes (as is the usual case), the major species for PMAA brushes was anhydride.…”

A self-healable fluorescence active hydrogel based on ionic block copolymers prepared via ring opening polymerization and xanthate mediated RAFT polymerization

“…From the Figure 9 it was observed that the hydrogels containing the BCP of different ionic block lengths showed good cell proliferation compared to the control sample. It is reported that the cell proliferation in some way depends on the surface hydrophilicity and the micro roughness of the surface 51 . As shown in Figure S5(b) on formation of the ionic BCPs the WCA value reduced from 78° (homo PCL) to 32° for cationic BCP and 36° for the anionic BCP.…”