Surface Modification of PDMS Based Microfluidic Systems by Tensides

Abstract

The material aspects of a polymer based microfluidic structure were characterised considering the compatibility of the system with bioanalytical applications. The polydimethylsiloxane (PDMS) based channel system is to be integrated in a full polymer photonic biosensor device developed within the European Union project P3SENS (FP7-ICT4-248304).This work is intended to define a modified material composition, which is appropriate to improve both the wettability and the non-specific protein binding characteristics of the PDMS significantly. Triton X-100 (Sigma-Aldrich) surfactant was added to the raw PDMS before polymerisation. The influence of the tenside was studied considering the polymerisation reaction, the surface characteristics and the functional applicability. To test the hydrodynamic behaviour and non-specific protein adsorption on the surfaces, phosphate buffered saline (PBS) solution and fluorescent labelled human serum albumin (HSA) was applied in a microfluidic capillary system.

Viscoelastic Separation and Concentration of Fungi from Blood for Highly Sensitive Molecular Diagnostics

“…Subsequently, the cured PDMS channels were peeled off from the replica mold, cut, and bonded on a glass slide with oxygen plasma (CUTE, Femto Science, South Korea). To minimize non-specific protein binding, surface modification was applied to the PDMS channel walls using Triton X-100 surfactant 63 .…”

Highly Conformable, Transparent Electrodes for Epidermal Electronics

“…In summary, according to Figure , the optical properties of a-PDMS are dramatically affected by the content of Triton X in PDMS, and the mechanical properties (Young’s modulus, failure strain, viscoelasticity, and adhesion force) are significantly affected by the T c of a-PDMS. According to the previous study by other researchers, Triton X have been used to modify the properties of the PDMS . The Triton X was added to the PDMS to modify its surface properties such as the surface energy and protein affinity .…”

PDMS microfluidics developed for polymer based photonic biosensors

“…The relative change in intensity after washing proved that irreversible HSA adsorption on the PDMS surface can be decreased by embedding 0.2 v/v% TX-100 or PDMS-PEO in the material. We can conclude that the irreversible protein adsorption on the PDMS surface can be decreased by almost 100% by this surface modification method [33].…”

“…The developing surface behaviour can be explained by the accumulation of the embedded molecules at the PDMS surface. The rapidly decreasing contact angle during the measurement indicates the increasing surface concentration of the modifying molecules at the PDMS / liquid interface [33,34,35]. The long term stability of the developed molecule layers were reported more than months by [11,12].…”

“…[31] The influence of the modification has been also studied and is found in literature [10,11,12,32,33]. Modified surface characteristics of our device were analyzed by contact angle measurement.…”