Surface modification of PCL‐TCP scaffolds improve interfacial mechanical interlock and enhance early bone formation: An <i>in vitro</i> and <i>in vivo</i> characterization

Yeo, Allen; Wong, W. J.; Khoo, Hock Hee; Teoh, Swee‐Hin

doi:10.1002/jbm.a.32366

Cited by 78 publications

(66 citation statements)

References 73 publications

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“…NaOH treatment of PCL was found to result in increased cell adhesion to the PCL films, consistent with studies using a multitude of different cell sources [34,35,44,45]. NaOH treatment causes hydrolysis of the ester bonds within the PCL films, thereby exposing carboxylic acid and hydroxyl groups on the surface which renders the surfaces more hydrophilic [46].…”

Section: Discussionsupporting

confidence: 83%

Conductive interpenetrating networks of polypyrrole and polycaprolactone encourage electrophysiological development of cardiac cells

et al. 2015

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Current conductive materials for use in cardiac regeneration are limited by cytotoxicity or cost in implementation. In this manuscript, we demonstrate for the first time the application of a biocompatible, conductive polypyrrole-polycaprolactone film as a platform for culturing cardiomyocytes for cardiac regeneration. This study shows that the novel conductive film is capable of enhancing cell-cell communication through the formation of connexin-43, leading to higher velocities for calcium wave propagation and reduced calcium transient durations among cultured cardiomyocyte monolayers. Furthermore, it was demonstrated that chemical modification of polycaprolactone through alkaline-mediated hydrolysis increased overall cardiomyocyte adhesion. The results of this study provide insight into how cardiomyocytes interact with conductive substrates and will inform future research efforts to enhance the functional properties of cardiomyocytes, which is critical for their use in pharmaceutical testing and cell therapy.

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Section: Discussionsupporting

confidence: 83%

Conductive interpenetrating networks of polypyrrole and polycaprolactone encourage electrophysiological development of cardiac cells

et al. 2015

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“…A greater number and size of micro pits with “channel‐like” indentations were detected after NaOH treatment. Interestingly, it did not affect the overall pore dimensions and interconnected honeycomb architecture even after 48 and 96 h of NaOH treatment 21…”

Section: Introductionmentioning

confidence: 91%

Surface modification of PCL‐TCP scaffolds in rabbit calvaria defects: Evaluation of scaffold degradation profile, biomechanical properties and bone healing patterns

Yeo

Wong

Teoh

2009

J Biomedical Materials Res

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Traditionally, polycaprolactone (PCL) based scaffolds tend to degrade at a slow rate. Pretreatment of polycaprolactone-20% tricalcium phosphate (PCL-TCP) scaffolds under alkaline conditions can be utilized to increase the degradation rate and improve mechanical properties. Three groups of PCL-TCP scaffolds with varying pretreatment exposures with sodium hydroxide (NaOH) were studied in a rabbit calvaria defect model and analyzed at 2, 4, 8, 12, and 24 weeks. (Group A: Untreated, Group B: 3 M NaOH/ 48 h and Group C: 3 M NaOH/96 h). Micro-CT analysis demonstrated that scaffolds with increased surface roughness (Groups B and C) showed a greater impact on the overall volume loss during the early healing period between 2 and 8 weeks as compared to the untreated group. In addition, greater bone formation was detected in NaOH treated scaffolds as compared to the untreated group throughout the experiment. Scaffolds with increased surface roughness generally reported higher push out test and compressive strength values from 4 to 8 weeks of early healing. Interestingly, the mechanical properties displayed a decline in values from 12 weeks onwards in the modified groups suggesting a favorable breakdown or weakening of PCL-TCP scaffolds tailored for replacement by new bone formation.

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“…Over the years, our group has developed PCL and PCLtricalcium phosphate (PCL-TCP) as viable biomaterials for TE applications, including skin, 13 bone, [14][15][16] and drug delivery. 17,18 By using a solvent-free approach of fused deposition modeling, PCL-based scaffolds have been fabricated with a variety of porosities ranging from 70%-90%.…”

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confidence: 99%

Brain Tissue Interaction with Three-Dimensional, Honeycomb Polycaprolactone-Based Scaffolds Designed for Cranial Reconstruction Following Traumatic Brain Injury

Choy

Nga

Lim

et al. 2013

Tissue Engineering Part A

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Following traumatic brain injury (TBI), resultant voids are unable to support injections of suspension treatments, leading to ineffective healing. Moreover, without a structure to support the large defect, the defect site suffers from mechanical instability, which may impair the healing process. Therefore, having a delivery vehicle that can temporarily fill and provide mechanical support to the defect site may alleviate the healing process. In this work, we reported for the first time, the inflammatory response of brain tissue with polycaprolactone (PCL) and PCLtricalcium phosphate (TCP) scaffolds designed and fabricated for cranial reconstruction. After cranial defects were created in Sprague-Dawley rats, PCL and PCL-TCP scaffolds were implanted for a period of 1 week and 1 month. Following histology and immunofluorescence staining with the ionized calcium binding adaptor molecule-1 (IBA-1), glial fibrillary acidic protein (GFAP), nestin, and neuronal nuclei (NeuN), results indicated that IBA-1-positive activated microglia were observed across all groups, and declined significantly by 1 month ( p < 0.05). Interestingly, IBA-1-positive microglia were significantly fewer in the PCL-TCP group ( p < 0.05), suggesting a relatively milder inflammatory response. A decrease in the number of GFAP-positive cells among all groups over time ( > 29%) was also observed. Initially, astrocyte hypertrophy was observed proximal to the TBI site (55% in PCL and PCL-TCP groups, 75% in control groups), but it subsided by 1 month. Proximal to the TBI site, nestin immunoreactivity was intense during week 1, and which reduced by 1 month across all groups. NeuN-positive neurons were shrunken proximal to the TBI site ( < 0.9 mm), 32% smaller in the PCL-TCP group and 27% smaller in the PCL group. Based on above data indicating the comparatively milder, initial inflammatory response of brain tissue to PCL-TCP scaffolds, it is suggested that PCL-TCP scaffolds have notable clinical advantages as compared to PCL scaffolds.

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Surface modification of PCL‐TCP scaffolds improve interfacial mechanical interlock and enhance early bone formation: An in vitro and in vivo characterization

Cited by 78 publications

References 73 publications

Conductive interpenetrating networks of polypyrrole and polycaprolactone encourage electrophysiological development of cardiac cells

Conductive interpenetrating networks of polypyrrole and polycaprolactone encourage electrophysiological development of cardiac cells

Surface modification of PCL‐TCP scaffolds in rabbit calvaria defects: Evaluation of scaffold degradation profile, biomechanical properties and bone healing patterns

Brain Tissue Interaction with Three-Dimensional, Honeycomb Polycaprolactone-Based Scaffolds Designed for Cranial Reconstruction Following Traumatic Brain Injury

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