Surface functionalization of ZnO nanoparticles with functionalized bovine serum albumin as a biocompatible photochemical and antimicrobial agent

Kanchana, U.S.; Mathew, Thomas

doi:10.1016/j.surfin.2021.101056

Cited by 12 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, they may be used as an adjuvant treatment to chemotherapeutic drugs to alleviate their toxic effects. [ 36–38 ] In the present study, all formulations were prepared based on BSA and successfully hybridized with Zn NPs according to our previously reported method. [ 24 ] The natural active compound, Ba, was used as a potential anticancer therapeutic agent.…”

Section: Discussionmentioning

confidence: 99%

Green synthesis of Zn nanoparticles and in situ hybridized with BSA nanoparticles for Baicalein targeted delivery mediated with glutamate receptors to U87‐MG cancer cell lines

Gharbavi

Johari

Ghorbani

et al. 2022

Applied Organom Chemis

View full text Add to dashboard Cite

Glioblastoma multiforme (GBM) is the most aggressive malignant tumor of the brain. It has different glutamate receptor types. So, these receptors can be a suitable target for GBM treatment. The current study investigated the anticancer effects of bovine serum albumin (BSA)‐Baicalein @Zn‐Glu nanostructure mediated‐GluRs in human glioblastoma U87 cells. BSA‐Ba@Zn‐Glu hybrid nanoparticles (NPs) were set and considered transporters for Baicalein (Ba) active compound delivery. BSA‐Ba@Zn‐Glu NPs were synthesized by a single‐step reduction process. The successful production was confirmed through transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT‐IR), and hemolysis test. The cytotoxic efficacy and apoptosis rate of the nanostructures on U87 glioblastoma cells were investigated by 3‐(4,5‐dimethylthialzol‐a‐yl)‐2,5 diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. The synthesized BSA‐Ba@Zn‐Glu nanostructures with a diameter of 142.40 ± 1.91 to 177.10 ± 1.87 nm and zeta potential of −10.57 ± 0.71 to −35.77 ± 0.60 mV are suitable for extravasation into tumor cells. The drug release from the BSA‐Ba@Zn NPs showed controlled and pH‐dependent behavior. In vitro results indicated that the BSA‐Ba@Zn‐Glu NPs significantly reduce cell viability and promote apoptosis of U87 cancer cells. It revealed the cytotoxic effect of the Baicalein and an increase in cellular uptake of nanoparticles by Glu receptors. Zn NPs were synthesized based on a green synthesis method. BSA NPs were used as a nano‐platform for Glu conjugation and Ba drug delivery. BSA‐Ba@Zn‐Glu NPs induce cytotoxicity and apoptosis in human brain cancer cells (U87) in a dose‐dependent manner. Finally, this nanostructure could be served in targeted drug delivery in vivo studies and applied along with other strategies such as X‐ray irradiation as combinational therapies in future studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Green synthesis of Zn nanoparticles and in situ hybridized with BSA nanoparticles for Baicalein targeted delivery mediated with glutamate receptors to U87‐MG cancer cell lines

Gharbavi

Johari

Ghorbani

et al. 2022

Applied Organom Chemis

View full text Add to dashboard Cite

show abstract

“…The peaks at 3303 cm −1 (N–H stretching), 1658 cm −1 (CO stretching) and 1540 cm −1 (CN stretching coupled with NH bending) were ascribed to BSA. [ 31 ] In addition, the peaks at 2932 cm −1 (CH stretching), 1494 cm −1 (CC stretching of benzenoid ring), 1291 cm −1 (CN stretching), 1126 cm −1 (CH in‐plane bending), and 805 cm −1 (CH out‐of‐plane bending) were attributed to PANI. [ 32 ] The results verified that the B@P 1 particles contained both BSA and PANI.…”

Section: Resultsmentioning

confidence: 99%

Acidity‐Activatable Nanoparticles with Glucose Oxidase‐Enhanced Photoacoustic Imaging and Photothermal Effect, and Macrophage‐Related Immunomodulation for Synergistic Treatment of Biofilm Infection

Dai

Mei

et al. 2022

Small

View full text Add to dashboard Cite

The pH‐responsive theragnostics exhibit great potential for precision diagnosis and treatment of diseases. Herein, acidity‐activatable nanoparticles of GB@P based on glucose oxidase (GO) and polyaniline are developed for treatment of biofilm infection. Catalyzed by GO, GB@P triggers the conversion of glucose into gluconic acid and hydrogen peroxide (H2O2), enabling an acidic microenvironment‐activated simultaneously enhanced photothermal (PT) effect/amplified photoacoustic imaging (PAI). The synergistic effects of the enhanced PT efficacy of GB@P and H2O2 accelerate biofilm eradication because the penetration of H2O2 into biofilm improves the bacterial sensitivity to heat, and the enhanced PT effect destroys the expressions of extracellular DNA and genomic DNA, resulting in biofilm destruction and bacterial death. Importantly, GB@P facilitates the polarization of proinflammatory M1 macrophages that initiates macrophage‐related immunity, which enhances the phagocytosis of macrophages and secretion of proinflammatory cytokines, leading to a sustained bactericidal effect and biofilm eradication by the innate immunomodulatory effect. Accordingly, the nanoplatform of GB@P exhibits the synergistic effects on the biofilm eradication and bacterial residuals clearance through a combination of the enhanced PT effect with immunomodulation. This study provides a promising nanoplatform with enhanced PT efficacy and amplified PAI for diagnosis and treatment of biofilm infection.

show abstract

“…18 In recent years, albumin-based nanoparticles have gained much attention owing to many advantages such as good biocompatibility, non-cytotoxicity, non-immunogenicity and biodegradability, hence are used in various applications such as receptor targeting, drug delivery vehicles, cancer therapy, antibacterial, antiproliferative, and so forth. [18][19][20][21][22][23] In biological systems, nanoparticles interact with serum proteins forming a dynamic corona of different affinities. Previous studies have shown that corona provides a unique identity to the nanoparticles affecting their biological or toxicological properties.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the significance of molecular interaction between sulphur doped zinc oxide nanoparticles and serum albumin using multispectroscopic approach

Mahanthappa,

Savanur,

Ramu

et al. 2023

J of Molecular Recognition

View full text Add to dashboard Cite

Ingenious nanomaterials with improved biocompatibility and multifunctional properties are gaining vital significance in biomedical applications, including advanced drug delivery and nanotheranostics. In a biological system, these nanoparticles interact with serum proteins forming a dynamic corona that affects their biological or toxicological properties producing undesirable effects. Thus, the current study focuses on the synthesis of sulphur‐doped zinc oxide nanoparticles (ZnO/S NPs) and characterizing their mechanism of interaction with serum proteins using multispectroscopic approach. ZnO/S NPs were synthesized by employing a co‐precipitation approach and characterized using various analytical techniques. The results of interaction studies demonstrated that ZnO/S NPs interact with serum albumins via the static quenching process. Analysis of thermodynamic parameters (ΔG, ΔH and ΔS) revealed that the binding process is spontaneous, exothermic and van der Waals force or hydrogen bonding plays a major role. The interaction of ZnO/S NPs with tyrosine residue in bovine serum albumin was established by synchronous fluorescence spectroscopy. In addition, the results of UV–visible, circular dichroism, Fourier transform infrared, Forster's resonance energy transfer theory and dynamic light scattering spectroscopic studies revealed that the ZnO/S NPs interact with albumin by inducing the conformational changes in secondary structure and reducing the α‐helix content.

show abstract

Surface functionalization of ZnO nanoparticles with functionalized bovine serum albumin as a biocompatible photochemical and antimicrobial agent

Cited by 12 publications

References 26 publications

Green synthesis of Zn nanoparticles and in situ hybridized with BSA nanoparticles for Baicalein targeted delivery mediated with glutamate receptors to U87‐MG cancer cell lines

Green synthesis of Zn nanoparticles and in situ hybridized with BSA nanoparticles for Baicalein targeted delivery mediated with glutamate receptors to U87‐MG cancer cell lines

Acidity‐Activatable Nanoparticles with Glucose Oxidase‐Enhanced Photoacoustic Imaging and Photothermal Effect, and Macrophage‐Related Immunomodulation for Synergistic Treatment of Biofilm Infection

Elucidating the significance of molecular interaction between sulphur doped zinc oxide nanoparticles and serum albumin using multispectroscopic approach

Contact Info

Product

Resources

About