Surface functionalization of tissue culture polystyrene plates with hydroxyapatite under body fluid conditions and its effect on differentiation behaviors of mesenchymal stem cells

Iijima, Kazutoshi; Suzuki, Ryo; Iizuka, Atsushi; Ueno-Yokohata, Hitomi; Kiyokawa, Nobutaka; Hashizume, Makoto

doi:10.1016/j.colsurfb.2016.08.020

Cited by 21 publications

(33 citation statements)

References 38 publications

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“…According to the inverse relationship, HA has the potential to suppress adipogenesis. Several previous studies have shown that HA coating or HA discs can inhibit the adipogenesis of different cell types . For instance, Zhang et al found that HA discs composed of natural HA powder inhibited adipogenic differentiation of mouse bone marrow derived MSCs by regulating several microRNAs in the adipogenesis pathway.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, Zhang et al found that HA discs composed of natural HA powder inhibited adipogenic differentiation of mouse bone marrow derived MSCs by regulating several microRNAs in the adipogenesis pathway. Iijima et al have reported that HA coating and synthetic HA discs suppressed the adipogenic differentiation of hMSCs through decreasing the formation of lipid droplets and the expression of PPARγ and C/EBPα. Similarly, Liu et al demonstrated that the mechanism of osteoinductivity of nHA coating on silk fibroin scaffolds could be inhibiting the adipogenesis via regulation of the PPAR signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

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The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

Yang

Huang

et al. 2018

J Biomedical Materials Res

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Due to its excellent biocompatibility, nanosized hydroxyapatite (nHA) has drawn much attention for various applications in biomedical fields. There are growing concerns about its biosecurity; however, little is known about its effects on adipogenesis. In the present study, nHA with three different sizes were synthesized, and the in vitro effects of nHA on cell proliferation and adipogenic differentiation of human mesenchymal stem cells (hMSCs) were investigated. The results clearly show that nHA does not affect the cell viability, the lipids droplets formation, triglyceride (TG) synthesis, and the expression of adipogenic marker genes/proteins of hMSCs at concentrations lower than 50 μg/mL. It is concluded that the adipogenic differentiation potential of hMSCs is not affected by nHA at noncytotoxic concentrations. These will provide a reference for the applications of nHA in biomedical fields. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1822-1831, 2018.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

Yang

Huang

et al. 2018

J Biomedical Materials Res

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“…For example, simulated body fluids can be used to facilitate growth of hydroxyapatite crystals on PS surfaces, with the goal of inducing osteogenic differentiation of mesenchymal stem cells. 35 However, the surface density can be difficult to control, and they can be easily removed from the surface with applied force. As well, a protein coating or plasma-activated PS surface is required to deposit acidic residues and coordinate nucleation of hydroxyapatite crystals.…”

Section: The Transformation Of Ps To Tcpsmentioning

confidence: 99%

The Evolution of Polystyrene as a Cell Culture Material

Lerman

Lembong

Muramoto

et al. 2018

Tissue Engineering Part B: Reviews

200

156

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Polystyrene (PS) has brought in vitro cell culture from its humble beginnings to the modern era, propelling dozens of research fields along the way. This review discusses the development of the material, fabrication, and treatment approaches to create the culture material. However, native PS surfaces poorly facilitate cell adhesion and growth in vitro. To overcome this, liquid surface deposition, energetic plasma activation, and emerging functionalization methods transform the surface chemistry. This review seeks to highlight the many potential applications of the first widely accepted polymer growth surface. Although the majority of in vitro research occurs on two-dimensional surfaces, the importance of three-dimensional (3D) culture models cannot be overlooked. The methods to transition PS to specialized 3D culture surfaces are also reviewed. Specifically, casting, electrospinning, 3D printing, and microcarrier approaches to shift PS to a 3D culture surface are highlighted. The breadth of applications of the material makes it impossible to highlight every use, but the aim remains to demonstrate the versatility and potential as both a general and custom cell culture surface. The review concludes with emerging scaffolding approaches and, based on the findings, presents our insights on the future steps for PS as a tissue culture platform.

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“…MSC differentiation into osteogenic tissue has been extensively investigated using scaffolds, such as Hap [51], nonwoven nano, and microfibrous scaffolds of polymers [52][53][54][55][56], and their composites. Factors affecting the osteogenic differentiation of MSCs are classified into two categories.…”

Section: Electrospun Silica Nonwoven Fabricsmentioning

confidence: 99%

Cell Scaffolds for Bone Tissue Engineering

Iijima

Otsuka

2020

Bioengineering

Self Cite

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Currently, well-known surgical procedures for bone defects are classified into four types: (1) autogenous bone graft transplantation, (2) allogeneic bone graft transplantation, (3) xenogeneic bone graft transplantation, and (4) artificial bone graft transplantation. However, they are often risky procedures and related to postoperative complications. As an alternative, tissue engineering to regenerate new bone often involves the use of mesenchymal stem cells (MSCs), derived from bone marrow, adipose tissues, and so on, which are cultured into three-dimensional (3D) scaffolds to regenerate bone tissue by osteoinductive signaling. In this manuscript, we provide an overview of recent treatment of bone defects and the studies on the creation of cell scaffolds for bone regeneration. Bone regeneration from bone marrow-derived mesenchymal stem cells using silica nonwoven fabric by the authors’ group were provided. Potential application and future direction of the present systems were also described.

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Surface functionalization of tissue culture polystyrene plates with hydroxyapatite under body fluid conditions and its effect on differentiation behaviors of mesenchymal stem cells

Cited by 21 publications

References 38 publications

The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

The Evolution of Polystyrene as a Cell Culture Material

Cell Scaffolds for Bone Tissue Engineering

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