Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells

Lee, Suk Won; Lee, Hong Jae; Lee, Jae Won; Kim, Kyung‐Hee; Kang, Jong-Ho; Lee, Myung Hyun; Lee, Sang Cheon

doi:10.1016/j.colsurfb.2015.08.011

Cited by 10 publications

(7 citation statements)

References 33 publications

(39 reference statements)

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“…Various studies have focused on Ti surface modications to promote osteogenic differentiation and inhibit bacterial colonisation. 31,32 However, before stem cells can arrive to the Ti implant surface to differentiate into osteoblasts and form bone, the inammatory response must be resolved since chronic inammation can inhibit bone-implant integration. 33,34 Therefore, controlling inammatory responses to implants is an important issue for medical devices owing to the negative effects of chronic inammation on device performance in vivo.…”

Section: Introductionmentioning

confidence: 99%

A comparative analysis of antibacterial properties and inflammatory responses for the KR-12 peptide on titanium and PEGylated titanium surfaces

Nie

Long

et al. 2017

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

A comparative analysis of antibacterial properties and inflammatory responses for the KR-12 peptide on titanium and PEGylated titanium surfaces

Nie

Long

et al. 2017

RSC Adv.

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“…Recently, thermal oxidation was conducted on a microgroove Ti surface at 700 °C for 3 h, which had the effect of increasing the oxide film and adding nano properties, and results of a similar level were obtained [27]. Finally, a recent study in which nanoparticles with loaded growth factors were attached to a microgroove Ti surface, inducing the release of sequential osteogenic growth factors, the result of a noteworthy increase in the promotion of osteogenic activity and osteoblastic differentiation was obtained, which has laid the foundation for the development of composite biomaterial surfaces with microgrooves [28]. The initial motivation of the present study was not merely the promotion of osteogenic activity and osteoblastic differentiation of a fixed level by way of microgrooves alone, as in the above studies, and, as expected, noteworthy results of promotion were obtained (figures 4 and 5).…”

Section: Discussionmentioning

confidence: 55%

Promotion of osteoblastic differentiation and osteogenic transcription factor expression on a microgroove titanium surface with immobilized fibronectin or bone sialoprotein II

Lee

et al. 2016

Biomed. Mater.

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We demonstrate that a composite surface of microgroove titanium (Ti) with immobilized fibronectin (FN) or bone sialoprotein II (BSP2) promotes osteoblastic differentiation and osteogenic transcription factor expression in human bone marrow-derived mesenchymal stem cells (MSCs). Comparisons made between smooth microgrooves, microgrooves with silanization and microgrooves with matrix protein (FN or BSP2)-immobilization Ti surfaces revealed a significant promotion of in vitro osteogenic activity and osteoblastic differentiation at various timelines of culture. An even more significant increase was verified on microgrooves with a matrix protein-immobilization Ti surface in 28 d time-dependent gene expression of the main osteogenic transcription factors, such as ARF4, FRA1, RUNX2, and OSX. As a result, a synergestic effect regarding the promotion of osteogenic transcription factor expression and osteoblastic differentiation in the matrix protein-microgroove Ti composite surface was confirmed. From a multiple regression analysis using various timelines of osteogenic culture as independent variables, day 13 was verified as the most prominent influential timeline for the promotion of osteoblastic differentiation induced by the matrix protein-microgroove Ti composite surface. The FN- or BSP2-microgroove Ti composite surface resulting from silanization can strongly induce the promotion of osteoblastic differentiation in human MSCs. The proposed surface is expected to be useful in the development of a variety of osteogenic biomaterial surfaces.

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“…Thus, Lee et al developed a system in which microgrooved titanium was functionalized through the addition of bone morphogenic protein‐2 (BMP‐2) and insulin‐like growth factor 1 (IGF‐1)‐loaded anionic nanoparticles to improve osteoblastic differentiation. Authors reported a sustained simultaneous release of both growth factors for up to 40 days and differentiation of MSC to osteoblasts as seen in osteoblastic markers genes (ALP, BSP2, COL1A1, Runx2, etc) increased expression . Therefore, the distribution and nanotopography of growth‐factor functionalized biomaterials are demonstrated to play a key role in the development on exciting and efficient therapies in the field of tissue regeneration.…”

Section: The Bio‐relevance Of Nanobiomaterials Surface Modificationsmentioning

confidence: 99%

Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine

Rana

Ramasamy

Leena

et al. 2016

Biotechnology Progress

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Stem cell-based approaches offer great application potential in tissue engineering and regenerative medicine owing to their ability of sensing the microenvironment and respond accordingly (dynamic behavior). Recently, the combination of nanobiomaterials with stem cells has paved a great way for further exploration. Nanobiomaterials with engineered surfaces could mimic the native microenvironment to which the seeded stem cells could adhere and migrate. Surface functionalized nanobiomaterial-based scaffolds could then be used to regulate or control the cellular functions to culture stem cells and regenerate damaged tissues or organs. Therefore, controlling the interactions between nanobiomaterials and stem cells is a critical factor. However, surface functionalization or modification techniques has provided an alternative approach for tailoring the nanobiomaterials surface in accordance to the physiological surrounding of a living cells; thereby, enhancing the structural and functional properties of the engineered tissues and organs. Currently, there are a variety of methods and technologies available to modify the surface of biomaterials according to the specific cell or tissue properties to be regenerated. This review highlights the trends in surface modification techniques for nanobiomaterials and the biological relevance in stem cell-based tissue engineering and regenerative medicine. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:554-567, 2016.

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Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells

Cited by 10 publications

References 33 publications

A comparative analysis of antibacterial properties and inflammatory responses for the KR-12 peptide on titanium and PEGylated titanium surfaces

A comparative analysis of antibacterial properties and inflammatory responses for the KR-12 peptide on titanium and PEGylated titanium surfaces

Promotion of osteoblastic differentiation and osteogenic transcription factor expression on a microgroove titanium surface with immobilized fibronectin or bone sialoprotein II

Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine

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