Surface Functionalization of Alumina Ceramic Foams with Organic Ligands

Abstract

Different anchoring groups have been studied with the aim of covalently binding organic linkers to the surface of alumina ceramic foams. The results suggested that a higher degree of functionalization was achieved with a pyrogallol derivative − as compared to its catechol analogue − based on the XPS analysis of the ceramic surface. The conjugation of organic ligands to the surface of these alumina materials was corroborated by DNP-MAS NMR measurements.

Functionalization of Microstructured Open-Porous Bioceramic Scaffolds with Human Fetal Bone Cells

“…However, the adhesion of human bone-derived cells onto the large pore scaffolds was not rapid enough to ensure the optimal cellularization of these scaffolds. Combining our previous observations that alumina-based scaffolds can be chemically functionalized with gallate-based ligands, 27 and that human fetal osteoblasts can be chemically functionalized using biocompatible click chemistry, 28 we showed here that the number of human fetal osteoblasts adhering to the scaffolds can be enhanced by their covalent binding to the scaffolds using an hetero-bifunctional ligand and biocompatible click-chemistry. Therefore, we show here that large-pore alumina-based scaffolds and primary human fetal cells derived from the fetal bones To define the diameters of the pores and pore-openings, SEM micrographs at low magnifications were analyzed (grey bars) using the software Linear Intercept, then the cumulative values were calculated (solid lines).…”

“…27 We have also previously shown that human fetal osteoblasts can be chemically functionalized using biocompatible click chemistry. 28 Thus, in order to enhance the adhesion of the fetal osteoblasts to the scaffold, we combined these two approaches by designing and synthesizing (Scheme 1) compound 5, an hetero-bifunctional ligand bearing a gallate functionality and an activated alkyne.…”

Holistic Approach of Swiss Fetal Progenitor Cell Banking: Optimizing Safe and Sustainable Substrates for Regenerative Medicine and Biotechnology

“…Phenotypic maturation in vivo was shown to not carry the immune privileges of therapeutic FPCs in rodent models (Hausherr et al, 2017). Chemical functionalization (e.g., click chemistry, bioorthogonal chemical reactions, covalent binding) of therapeutic cell surfaces allows optimal conjugation with bioengineered scaffolds, while maintaining and optimizing cellular viability, adhesion, persistence, and function (Borcard et al, 2011(Borcard et al, , 2012Comas et al, 2012;Krauss Juillerat et al, 2012). Optimal mechanical properties and efficient vascularization capacity of implanted constructs are essential, while biodegradable hydrogels may enable local cell maintenance (Tenorio et al, 2011;Amini et al, 2012).…”

Surface Modification of Biomaterials for Conjugation with Human Fetal Osteoblasts

“…For that purpose, the ligand 8 combining a pyrrogallol moiety for attachment to the inorganic matrix of the biomaterial [14] and DIBO was used. The hydroxyapatite (HA) and β-tricalcium phosphate (TCP) biomaterials were functionalized by incubation with a 0, 5 or 10 µM solution of 8 for 24 h. acid AHA.…”

“…No decrease of the cell survival was observed compared to the non-functionalized cells, suggesting that this modification is not toxic for FsOs. A second chemical ligand 8 containing a pyrrogallol moiety for the binding to the biomaterial discs [14] and a cyclic activated alkyne for the covalent linkage to FsOs was synthesized in reasonable yield. The HA and TCP biomaterials were then functionalized at different concentrations with ligand 8 and AHA-labeled FsOs were added.…”