Surface-enhanced Raman spectroscopy introduced into the International Standard Organization (ISO) regulations as an alternative method for detection and identification of pathogens in the food industry

Witkowska, Evelin; Korsak, Dorota; Kowalska, Aneta; Księżopolska-Gocalska, Monika; Niedziółka‐Jönsson, Joanna; Roźniecka, Ewa; Michałowicz, Weronika; Albrycht, Paweł; Podrażka, Marta; Hołyst, Robert; Waluk, Jacek; Kamińska, Agnieszka

doi:10.1007/s00216-016-0090-z

Cited by 51 publications

(26 citation statements)

References 40 publications

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“…The presence of 11 coincident bands was observed. These bands were assigned to the corresponding biological molecules, as previously reported by other authors ( Assaf et al, 2014 ; Witkowska et al, 2017 ; Table 3 ). Phenotypic changes in Salmonella Typhimurium cells treated with NPs were analyzed.…”

Section: Resultssupporting

confidence: 63%

Biogenic Silver Nanoparticles as a Strategy in the Fight Against Multi-Resistant Salmonella enterica Isolated From Dairy Calves

Estevez

Casaux

Fraga

et al. 2021

Front. Bioeng. Biotechnol.

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Infectious diseases are one of the most important health problems worldwide, one of the main causes being the development of multi-resistant microorganisms. Likewise, the zoonotic potential of some pathogens and their ability to transfer resistance mechanisms, reduce the therapeutic options in both humans and animals. Salmonella enterica is an important pathogen that affects a wide range of animal species and humans, being Salmonella Typhimurium one of the most frequent serotypes affecting cattle, causing enteritis, diarrhea, and septicemia. The search for alternative therapeutic approaches has gained importance since the emergence of multidrug resistance to antibiotics and periodic outbreaks of salmonellosis. In this sense, the discovery of new drugs and the development of new strategies, such as the use of nanoparticles with antimicrobial activity, are very promising. The aim of this work was the extracellular production of biogenic silver nanoparticles using fungal extracts and the evaluation of their antimicrobial activity against resistant and multi-resistant Salmonella Typhimurium strains. We here demonstrated the potential of the biogenic nanoparticles as effective bacteriostatic and bactericidal agents for use in biomedical applications. In addition, Confocal Raman Microscopy and Atomic Force Microscopy were used to advance the understanding of the antimicrobial mechanism of biogenic nanoparticles against these pathogenic strains, the results of which suggested that the nanoparticles produced damage in several bacterial cell structures.

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Section: Resultssupporting

confidence: 63%

Biogenic Silver Nanoparticles as a Strategy in the Fight Against Multi-Resistant Salmonella enterica Isolated From Dairy Calves

Estevez

Casaux

Fraga

et al. 2021

Front. Bioeng. Biotechnol.

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“…These bacteria are listed in Commission Regulation (EC) No. 2073/2005 on microbiological criteria for foodstuffs and were successfully detected via the SERS method from different food matrices [14]. It was also proven that SERS discrimination of two bacterial species belonging to one genus ( L. monocytogenes and L. ivanovii ) is possible [14].…”

Section: Introductionmentioning

confidence: 99%

“…2073/2005 on microbiological criteria for foodstuffs and were successfully detected via the SERS method from different food matrices [14]. It was also proven that SERS discrimination of two bacterial species belonging to one genus ( L. monocytogenes and L. ivanovii ) is possible [14]. However, bacteria identification at the strain level is also an important issue as some strains, even within the same species, may be very virulent and pathogenic for humans while others are not.…”

Section: Introductionmentioning

confidence: 99%

Strain-level typing and identification of bacteria – a novel approach for SERS active plasmonic nanostructures

et al. 2018

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One of the potential applications of surface-enhanced Raman spectroscopy (SERS) is the detection of biological compounds and microorganisms. Here we demonstrate that SERS coupled with principal component analysis (PCA) serves as a perfect method for determining the taxonomic affiliation of bacteria at the strain level. We demonstrate for the first time that it is possible to distinguish different genoserogroups within a single species, Listeria monocytogenes, which is one of the most virulent foodborne pathogens and in some cases contact with which may be fatal. We also postulate that it is possible to detect additional proteins in the L. monocytogenes cell envelope, which provide resistance to benzalkonium chloride and cadmium. A better understanding of this infectious agent could help in selecting the appropriate pharmaceutical product for enhanced treatment. Graphical abstractᅟ Electronic supplementary materialThe online version of this article (10.1007/s00216-018-1153-0) contains supplementary material, which is available to authorized users.

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“…The fingerprint identification characteristics of SERS technology has allowed it to become an important and effective method for distinguishing closely related bacteria growing on the same solid or liquid medium. [135] The application of SERS for clinical bacterial detection and identification in human body fluids has gained much attention. [136,137] In recent years, there have been a series of reports about the clinical bacterial detection and identification of SERS.…”

Section: Application Of Bacterial Detection In Clinical and Food Safetymentioning

confidence: 99%

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

et al. 2020

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The rapid, highly sensitive, and accurate detection of bacteria is the focus of various fields, especially food safety and public health. Surface-enhanced Raman spectroscopy (SERS), with the advantages of being fast, sensitive, and nondestructive, can be used to directly obtain molecular fingerprint information, as well as for the on-line qualitative analysis of multicomponent samples. It has therefore become an effective technique for bacterial detection. Within this progress report, advances in the detection of bacteria using SERS and other compatible techniques are discussed in order to summarize its development in recent years. First, the enhancement principle and mechanism of SERS technology are briefly overviewed. The second part is devoted to a label-free strategy for the detection of bacterial cells and bacterial metabolites. In this section, important considerations that must be made to improve bacterial SERS signals are discussed. Then, the label-based SERS strategy involves the design strategy of SERS tags, the immunomagnetic separation of SERS tags, and the capture of bacteria from solution and dye-labeled SERS primers. In the third part, several novel SERS compatible technologies and applications in clinical and food safety are introduced. In the final part, the results achieved are summarized and future perspectives are proposed.

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Surface-enhanced Raman spectroscopy introduced into the International Standard Organization (ISO) regulations as an alternative method for detection and identification of pathogens in the food industry

Cited by 51 publications

References 40 publications

Biogenic Silver Nanoparticles as a Strategy in the Fight Against Multi-Resistant Salmonella enterica Isolated From Dairy Calves

Biogenic Silver Nanoparticles as a Strategy in the Fight Against Multi-Resistant Salmonella enterica Isolated From Dairy Calves

Strain-level typing and identification of bacteria – a novel approach for SERS active plasmonic nanostructures

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

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