Surface‐enhanced Raman scattering study of human serum on PVAAg nanofilm prepared by using electrostatic self‐assembly

Liu, Renming; Xing-fa, ZI; Kang, Yipu; Min-zhen, SI; Wu, Yanchun

doi:10.1002/jrs.2665

Cited by 33 publications

(32 citation statements)

References 36 publications

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“…1, along with their difference. Both spectra present features already reported by several groups [16,18,20,21,36], using various substrates, and in particular, the bands due to uric acid and hypoxanthine [18,19,37].…”

Section: Resultssupporting

confidence: 52%

SERS analysis of serum for detection of early and locally advanced breast cancer

et al. 2015

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In this contribution, we investigated whether surface-enhanced Raman scattering (SERS) of serum can be a candidate method for detecting "luminal A" breast cancer (BC) at different stages. We selected three groups of participants aged over 50 years: 20 healthy women, 20 women with early localized small BC, and 20 women affected by BC with lymph node involvement. SERS revealed clear spectral differences between these three groups. A predictive model using principal component analysis (PCA) and linear discriminant analysis (LDA) was developed based on spectral data, and its performance was estimated with cross-validation. PCA-LDA of SERS spectra could distinguish healthy from BC subjects (sensitivity, 92 %; specificity, 85 %), as well as subjects with BC at different stages, with a promising diagnostic performance (sensitivity and specificity, ≥80 %; overall accuracy, 84 %). Our data suggest that SERS spectroscopy of serum, combined with multivariate data analysis, represents a minimally invasive, easy to use, and fast approach to discriminate healthy from BC subjects and even to distinguish BC at different clinical stages.

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Section: Resultssupporting

confidence: 52%

SERS analysis of serum for detection of early and locally advanced breast cancer

et al. 2015

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“…3c) exhibits similar vibrational frequencies as reported for the SERS spectra of plasma due to other plasmonically enhancing substrates excited at 785 nm, including enriched Au 5 and Ag 3, 4, 6, 26 colloid solutions and a PVA-Ag nanoparticle films. 27 As seen in Fig. 3, the SERS spectrum of fresh whole blood (Fig.…”

Section: Resultsmentioning

confidence: 86%

Surface-Enhanced Raman Scattering of Whole Human Blood, Blood Plasma, and Red Blood Cells: Cellular Processes and Bioanalytical Sensing

2012

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SERS spectra of whole human blood, blood plasma, and red blood cells on Au nanoparticle SiO 2 substrates excited at 785 nm have been observed. For the sample preparation procedure employed here, the SERS spectrum of whole blood arises from the blood plasma component only. This is in contrast to the normal Raman spectrum of whole blood excited at 785 nm and open to ambient air, which is exclusively due to the scattering of oxyhemoglobin. The SERS spectrum of whole blood shows a storage time dependence that is not evident in the non-SERS Raman spectrum of whole blood. Hypoxanthine, a product of purine degradation, dominates the SERS spectrum of blood after ∼10−20 h of storage at 8 °C. The corresponding SERS spectrum of plasma isolated from the stored blood shows the same temporal release of hypoxanthine. Thus, blood cellular components (red blood cells, white blood cells, and/or platelets) are releasing hypoxanthine into the plasma over this time interval. The SERS spectrum of red blood cells (RBCs) excited at 785 nm is reported for the first time and exhibits well-known heme group marker bands as well as other bands that may be attributed to cell membrane components or protein denaturation contributions. SERS, as well as normal Raman spectra, of oxy-and met-RBCs are reported and compared. These SERS results can have significant impact in the area of clinical diagnostics, blood supply management, and forensics.

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“…It is found that there are no or weak Raman signals from sodium citrate adsorbed on the Al substrate and PVA‐Ag nanofilms prepared with the reaction time of 20 s. However, large enhancements for the Raman scattering of sodium citrate adsorbed on PVA‐Ag GPs prepared with the reaction times of 40 and 60 s are observed, indicating large Raman scattering enhancements of the PVA‐Ag GPs prepared with the reaction times of 40 and 60 s. The normal Raman spectrum of powder sodium citrate is demonstrated in Fig. S3 (Supporting Information), and the main experimental Raman and SERS wavenumbers and their assignments are displayed in Table S1 based on our previous works . To verify the reproducibility of the SERS spectra on the basis of this substrate, we performed error bars for Raman band areas at 705, 1000, 1237 and 1390 cm −1 in the SERS spectra of (b), (c) and (d) shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Surface‐enhanced Raman scattering‐based approach for DNA detection at low concentrations via polyvinyl alcohol‐protected silver grasslike patterns

Liu

Zhu

Min-zhen

et al. 2011

J Raman Spectroscopy

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A simple method is demonstrated to detect DNA at low concentrations on the basis of surface‐enhanced Raman scattering (SERS) via polyvinyl alcohol‐protected silver grasslike patterns (PVA‐Ag GPs) grown on the surface of the common Al substrate. By the SERS measurements of sodium citrate and thymine, the PVA‐Ag GPs are shown to be an excellent SERS substrate with good activity, stability and reproducibility. With the use of the tested molecule of thymine, the enhancement factor of the PVA‐Ag GPs is up to ~1.4 × 108. The PVA‐Ag GPs are also shown to be an excellent SERS substrate with good biocompatibility for DNA detection, and the detection limit is down to ~10−5 mg/g. Meanwhile, the assignations of the Raman bands and the adsorption behaviors of the DNA molecules are also analyzed. In this work, the geometry optimization and the wavenumber analysis of adenine–Ag and guanine–Ag complexes for the ground states are performed using density functional theory, B3LYP functional and the LanL2DZ basis set. The transition energies and the oscillator strengths of adenine–Ag and guanine–Ag for the lowest six singlet excited states were calculated by using the time‐dependent density functional theory method with the same functional and basis set. The results show that the charge transfer in the adenine–Ag and guanine–Ag complexes should be the chemical factor for the SERS of the DNA molecules. Lastly, this method may be employed in large‐scale preparation of substrates that have been widely applied in the Raman analysis of DNA because the fabrication process is simple and inexpensive. Copyright © 2011 John Wiley & Sons, Ltd.

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Surface‐enhanced Raman scattering study of human serum on PVAAg nanofilm prepared by using electrostatic self‐assembly

Cited by 33 publications

References 36 publications

SERS analysis of serum for detection of early and locally advanced breast cancer

SERS analysis of serum for detection of early and locally advanced breast cancer

Surface-Enhanced Raman Scattering of Whole Human Blood, Blood Plasma, and Red Blood Cells: Cellular Processes and Bioanalytical Sensing

Surface‐enhanced Raman scattering‐based approach for DNA detection at low concentrations via polyvinyl alcohol‐protected silver grasslike patterns

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