Surface-Enhanced Raman Scattering of Dopamine on Self-Assembled Gold Nanoparticles

Abstract

Dopamine, a potent neurotransmitter in the brain, influences a variety of motivated behaviors and plays a major role in Parkinson's disease. In this study, the Raman signal of dopamine was detected on a fabricated nanoparticle-immobilized glass surface by surface-enhanced raman spectroscopy (SERS). Amine-modified glass was prepared by the self-assembly of amine-terminated silane on substrate, followed by the deposition of gold nanoparticles. The gold nanoparticles deposited on the glass surface were functionalized by anti-dopamine or dopamine. The antigen-dopamine was captured by antibody-assembled gold substrate and detected by SERS. The optical properties and morpology of the glass substrate with immobilized gold nanoparticles were analyzed by scanning electron microscopy and UV-VIS absorption spectroscopy. The Raman spectrum of dopamine displayed broad bands at 1267, 1331, 1158, 1478, 1578 and 1584 cm(-1). The strongest peaks in the spectra (at 1267 and 1478 cm(-1)) were identified as phenolic carbon-oxygen and phenyl C=C stretches, respectively. A working curve of the SERS signal constructed from cathecol ring vibration versus antigen-dopamine concentration was obtained at 1478 cm(-1), and the non-optimized detection limit for anti-dopamine surface antigen was as low as 1 ng/ml. These results suggest that SERS-based immunosensor can be a promising tool for the detection and screening of neurotransmitters.

Recent Advances in Electrochemical and Optical Sensing of Dopamine

“…They obtained reproducible wavelength-scanned SERS measurements in a wide spectral range using the same substrate. Raman signal of DA was detected based on antigen-antibody interactions with LOD of 1 ng/mL [307]. The developed sensor by Lim et al using a CS-Au nanoshell as a SERS substrate was very simple [308].…”

Gold nanoparticle based optical and electrochemical sensing of dopamine

“…The use of AuNPs material for the development of SERS substrate provides a better performing, more sensitive and cost-effective substrates for SERS applications especially in sensor application. Jeung-Hee An et al have fabricated a substrate based on AuNPs-immobilized glass surface for the detection of DA using SERS [21]. The preparation of this substrate involves the modification of the glass by self-assembly of amine-terminated silane, followed by the deposition of AuNPs before undergo functionalization by anti-DA or DA.…”

Dopamine-Induced Growth of Au and Ag Nanoparticles on ITO Substrate and Their Application in PCPDTBT-Based Polymer Solar Cell

“…Here, the same SILAR process was used on glass slides instead of ITO substrates with additional SILAR cycles, in order to increase the amount of adhered metal NPs and thus enhance the SERS signal. Figure 5 shows the SERS spectra of [24,25]. The relatively small peak at around 1450 cm −1 was assigned to the ring stretch vibration contributed mainly from the stretching of the carbon-carbon bond to which the oxygen is attached.…”