Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI TOF-MS) and ProteinChip® technology in proteomics research

Seibert, Volker; Wiesner, Andreas; Buschmann, Thomas; Meuer, Jörn

doi:10.1016/j.prp.2004.01.010

Cited by 114 publications

(76 citation statements)

References 29 publications

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“…The ProteinChip ® reader is an LDI-TOF/MS unit equipped with a laser source. Upon activation of the laser, the sample becomes irradiated, and is then subjected to DI, which liberates gaseous ions from the ProteinChip ® array (11). Next, these gaseous ions enter the TOF/MS region of the instrument, which measures the m/z of each protein based on its velocity through an ion chamber (11).…”

Section: Seldi Techniquementioning

confidence: 99%

“…Upon activation of the laser, the sample becomes irradiated, and is then subjected to DI, which liberates gaseous ions from the ProteinChip ® array (11). Next, these gaseous ions enter the TOF/MS region of the instrument, which measures the m/z of each protein based on its velocity through an ion chamber (11). Subsequent signal processing is accomplished by a high-speed analog-to-digital converter linked to a personal computer, whereby the detected proteins are displayed as a series of peaks (11).…”

Section: Seldi Techniquementioning

confidence: 99%

“…In addition, since SELDI-TOF/MS requires minimal quantities of a biological sample to generate an accurate protein profile in a relatively short period of time, it is suitable for mapping protein profiles in samples derived from healthy and diseased individuals in order to identify differential protein expression patterns between the two groups (10). The results of a SELDI-TOF/MS analysis are usually presented as a list of proteins that are upregulated or downregulated in healthy versus diseased subjects (11).…”

Section: Introductionmentioning

confidence: 99%

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Novel possibilities in the study of the salivary proteomic profile using SELDI-TOF/MS technology

et al. 2016

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Abstract. There is currently an increasing interest in exploring human saliva to identify salivary diagnostic and prognostic biomarkers, since the collection of saliva is rapid, non-invasive and stress-free. Diagnostic tests on saliva are common and cost-effective, particularly for patients who need to monitor their hormone levels or the effectiveness of undergoing therapies. Furthermore, salivary diagnostics is ideal for surveillance studies and in situations where fast results and inexpensive technologies are required. The most important constituents of saliva are proteins, the expression levels of which may be modified due to variations of the cellular conditions. Therefore, the different profile of proteins detected in saliva, including their absence, presence or altered levels, is a potential biomarker of certain physiological and/or pathological conditions. A promising novel approach to study saliva is the global analysis of salivary proteins using proteomic techniques. In the present study, surface-enhanced laser desorption/ionization-time-of-flight/mass spectrometry (SELDI-TOF/MS), one of the most recent proteomic tools for the identification of novel biomarkers, is reviewed. In addition, the possible use of this technique in salivary proteomic studies is discussed, since SELDI technology combines the precision of matrix-assisted laser desorption/ionization-TOF/MS proteomic analysis and the high-throughput nature of protein array analysis.

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Section: Seldi Techniquementioning

confidence: 99%

Section: Seldi Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel possibilities in the study of the salivary proteomic profile using SELDI-TOF/MS technology

et al. 2016

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“…14 The preparative and analytic processes can be sped up considerably with automation. 29,33 What can we exactly obtain with this method? A spectrum profile that shows hundreds of bars.…”

Section: The Proteome Of the Human Serum And Its Examinationmentioning

confidence: 99%

“…serum and urine) cannot be directly compared quantitatively. 29 In their answers the authors 23 have accepted the criticism and declared it necessary that the WHO, the Human Proteome Organization (HUPO), the Food and Drug Administration, the Red Cross, etc. work out international standards.…”

Section: Criticism Of Biological Mass Spectrometrymentioning

confidence: 99%

A path or a new road in laboratory diagnostics? Biological mass spectrometry: Facts and perspectives

Elek

Lapis

2006

Pathol. Oncol. Res.

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Systems Biology: Integrating ‘‐Omics'‐Oriented Approaches to Determine Foodborne Microbial Toxins

Singh¹,

Nagaraj²,

Gabani³

2009

General, Applied and Systems Toxicology

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The possible origins of microbial toxins vary widely, and detection of these toxins in different food matrices is a major challenge for food industries and regulatory agencies. New methodologies are needed to quickly and precisely detect traces of micro‐organisms and their toxic metabolites. In post‐genomics era, systems biology approaches, ranging from genomic sequencing to transcriptomics, proteomics and metabolomic profiling, may be an effective platform for developing tests to identify a variety of toxins in field applications; multiple functional ‘‐omics’ could be combined into a system‐wide approach for detecting toxins, which may also be useful in the study of microbial pathogenesis. Advances in systems biology are addressed in the current article, as well as possible uses of these high‐throughput platforms to ensure food and feed safety.

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Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI TOF-MS) and ProteinChip® technology in proteomics research

Cited by 114 publications

References 29 publications

Novel possibilities in the study of the salivary proteomic profile using SELDI-TOF/MS technology

Novel possibilities in the study of the salivary proteomic profile using SELDI-TOF/MS technology

A path or a new road in laboratory diagnostics? Biological mass spectrometry: Facts and perspectives

Systems Biology: Integrating ‘‐Omics'‐Oriented Approaches to Determine Foodborne Microbial Toxins

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