A path or a new road in laboratory diagnostics? Biological mass spectrometry: Facts and perspectives

Elek, Gábor; Lapis, K.

doi:10.1007/bf02893366

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…These properties notwithstanding, 2DE allows for the isolation of proteins in microgram amounts for further structural analysis by mass spectrometry (MS) or protein sequencing. MS has assumed a key role for identification and precise quantification of proteins from complex samples, thereby enabling large-scale and high-throughput characterization of the human proteome 8,9 . Because of its simplicity, accuracy, high resolution and sensitivity, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is useful for the identification of proteins by peptide mass fingerprinting.…”

Section: Introductionmentioning

confidence: 99%

Inverse changes in plasma tetranectin and titin levels in patients with type 2 diabetes mellitus: a potential predictor of acute myocardial infarction?

Rahim

Ahmad

et al. 2018

Acta Pharmacol Sin

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An early intervention using biomarkers to predict acute myocardial infarction (AMI) will effectively reduce global heart attack incidence, particularly among high-risk patients with type 2 diabetes mellitus (T2DM). This study attempted to identify potential biomarkers by detecting changes in the levels of plasma proteins in T2DM patients following onset of AMI in comparison with those without AMI. Volunteer T2DM patients without AMI (control; n=10) and T2DM patients with AMI (n=10) were recruited. Plasma samples from these patients were evaluated via two-dimensional gel electrophoresis (2DE) to screen for proteins with level changes between the two groups. The abundance of spots on gel images was analyzed using Progenesis SameSpots and subjected to false discovery rate (FDR) analysis. Protein spots with statistically significant changes of at least 1.5 fold were selected for mass spectrometry (MS) analysis. Due to strong cardiac connections, tetranectin and titin were evaluated by enzymelinked immunosorbent assay (ELISA). The adjusted P-values and fold changes between the two groups resulted in identification of 34 protein spots with significantly altered abundance. Upon MS analysis, 17 plasma proteins were identified: tetranectin, titin, clusterin, haptoglobin, myosin-13, zinc fnger protein 445, DNA repair protein RAD50, serum albumin, apolipoprotein A-IV, caspase-6, aminoacyl tRNA synthase complex-interacting multifunctional protein 1, serotransferrin, retinol-binding protein 4, transthyretin, alpha-1-antitrypsin, apolipoprotein A-I and serum amyloid A. Comparable patterns of changes in tetranectin and titin between the control and AMI groups were confirmed using ELISA. In summary, tetranectin and titin in plasma appeared to be closely associated with the onset of AMI among T2DM patients and can be used as potential biomarkers for prediction of a cardiac event, though this requires validation in a prospective cohort study.

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Section: Introductionmentioning

confidence: 99%

Inverse changes in plasma tetranectin and titin levels in patients with type 2 diabetes mellitus: a potential predictor of acute myocardial infarction?

Rahim

Ahmad

et al. 2018

Acta Pharmacol Sin

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“…Among other technologies, the development of genomics and proteomics have been suggested to play a role in clinical decision making in cancer screening, diagnosis and monitoring [14,15]. Application of protein microarray to exhaled breath condensate may provide a new perspective for the assessment of different types of lung cancer.…”

Section: Discussionmentioning

confidence: 99%

Differential Cytokine Pattern in the Exhaled Breath of Patients with Lung Cancer

Kullmann

Barta

Csiszér

et al. 2008

Pathol. Oncol. Res.

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Tumour cells may alter the protein pattern of biological samples resulting in specific differences that may aid diagnosis and treatment. In this pilot study we tested the cytokine pattern of exhaled breath condensate of patients with lung cancer. Breath condensates collected from 50 smoking patients with lung cancer and 25 smokers without clinical or radiological sign of a pulmonary tumour but having co-morbidities with similar severity as those with lung cancer were pooled for antibody microarray analysis testing 120 cytokines in parallel. Every cytokine on the array gave a signal in both groups. Nine cytokines including eotaxin, FGFs, IL-10 and MIP-3 were present with more than two-fold difference between the two groups. Large number of cytokines is present in the exhaled breath. Further analysis of specific differences associated with lung cancer may have clinical importance.

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Proteome analysis in plant stress research: a review

Vítámvás¹,

Kosová²,

Prášil³

2007

CZECH J GENET PLANT

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A path or a new road in laboratory diagnostics? Biological mass spectrometry: Facts and perspectives

Cited by 4 publications

References 32 publications

Inverse changes in plasma tetranectin and titin levels in patients with type 2 diabetes mellitus: a potential predictor of acute myocardial infarction?

Inverse changes in plasma tetranectin and titin levels in patients with type 2 diabetes mellitus: a potential predictor of acute myocardial infarction?

Differential Cytokine Pattern in the Exhaled Breath of Patients with Lung Cancer

Proteome analysis in plant stress research: a review

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