Surface-Enhanced Hyper-Raman Spectra of Adenine, Guanine, Cytosine, Thymine, and Uracil

Madzharova, Fani; Heiner, Zsuzsanna; Gühlke, Marina; Kneipp, Janina

doi:10.1021/acs.jpcc.6b02753

Cited by 158 publications

(217 citation statements)

References 46 publications

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“…prerequisite for the molecule for taking part in SEHRS (and also SERS) -depends critically on the surface charge and charge of the molecule. 23,101 As an example, in spectra of para-mercaptobenzoic acid, measured with hydroxylamine-stabilized silver nanoparticles, a reduction in SEHRS enhancement by a factor of 2-3 at very low and very high pH was observed. 22 In 532 nm excited SERS spectra with the same samples, the effect was not present.…”

Section: Sehrs With Nanoparticles and Nanoaggregatesmentioning

confidence: 99%

“…10,[22][23][24]42,44,60,68 A few groups report SEHRS spectra on immobilized or lithographically fabricated nanostructures. 64,69 As a first real biological application, SEHRS spectra were collected to analyze intrinsic biochemical information from nanometer-scaled volumes in dry-fixed individual culture cells.…”

Section: 67mentioning

confidence: 99%

“…44,45,83,86 Experiments using one individual wavelength were also reported using more compact, turn-key mode-locked Nd:YAG lasers with pulse durations of a few picoseconds (1-10 ps) with relatively high peak powers. 17,20,[22][23][24]64,70,82,85 Excitation of SEHRS in the near infrared can also be combined with excitation of one-photon SERS in the near infrared in the identical sample. 22,44,64 In spite of their relatively low peak intensities, tunable synchronously pumped optical parametric oscillators (OPOs) with signals between 0.72 and 0.99 mm or with idler pulses from 1.15 up to 2 mm can provide possibilities for excitation of SEHRS.…”

Section: Excitation Pulse Parametersmentioning

confidence: 99%

“…For example, nanoaggregates formed by citrate reduced silver nanoparticles yield higher overall SEHRS intensities than nanoaggregates from hydroxylamine reduced silver nanoparticles, although exactly the opposite is found for SERS. 23 In silver nanoaggregates, enhancement was discussed for individual nanostructures, assessing the plasmonic properties as a function of polarization. 72,73 Such experiments provide evidence that it is important to design suitable plasmonic materials for SEHRS, and to further understand the factors that influence SEHRS enhancement in the experiments.…”

Section: Focal Volumes and Amount Of Probed Samplementioning

confidence: 99%

“…22,23,31,48,61 In experiments where the detection frequency range is identical in the one-and two-photon excited case, SERS and SEHRS spectra can be collected quasi-parallel in the same experimental set-up. 20,23,44,53,[62][63][64] Simultaneous detection of SEHRS and SERS when using the same excitation wavelength for both one-and two-photon SERS was also reported. 44,65 For collecting the scattered light, both forward and backward directions are used.…”

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confidence: 99%

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Surface enhanced hyper Raman scattering (SEHRS) and its applications

2017

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Surface enhanced hyper Raman scattering (SEHRS) and its applicationsSurface enhanced hyper Raman scattering (SEHRS), spontaneous, two-photon excited, plasmon-enhanced Raman scattering, provides a wealth of vibrational information that can be useful in many directions of spectroscopy. As featured in:See Being regarded as a non-linear analogue of surface enhanced Raman scattering (SERS), SEHRS shares most of its properties, but also has additional characteristics. They include complementary spectroscopic information resulting from different selection rules and a stronger enhancement due to the non-linearity in excitation. In practical spectroscopy, this can translate to advantages, which include a high selectivity when probing molecule-surface interactions, the possibility of probing molecules at low concentrations due to the strong enhancement, and the advantages that come with excitation in the near-infrared. In this review, we give examples of the wealth of vibrational spectroscopic information that can be obtained by SEHRS and discuss work that has contributed to understanding the effect and that therefore provides directions for SEHRS spectroscopy. Future applications could range from biophotonics to materials research.

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Section: Sehrs With Nanoparticles and Nanoaggregatesmentioning

confidence: 99%

Section: 67mentioning

confidence: 99%

Section: Excitation Pulse Parametersmentioning

confidence: 99%

Section: Focal Volumes and Amount Of Probed Samplementioning

confidence: 99%

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Surface enhanced hyper Raman scattering (SEHRS) and its applications

2017

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High‐Density Plasmonic Nanopores for DNA Sensing at Ultra‐Low Concentrations by Plasmon‐Enhanced Raman Spectroscopy

Iarossi

Darvill

Hubarevich

et al. 2023

Adv Funct Materials

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Solid‐state nanopores are implemented in new and promising platforms that are capable of sensing fundamental biomolecular constituents at the single‐molecule level. However, several limitations and drawbacks remain. For example, the current strategies based on both electrical and optical sensing suffer from low analyte capture rates and challenging nanofabrication procedures. In addition, their limited discrimination power hinders their application in the detection of complex molecular constructs. In contrast, Raman spectroscopy has recently demonstrated the ability to discriminate both nucleotides and amino acids. Herein, a plasmonic nanoassembly is proposed supporting nanopores at high density, in the order of 100 pores per µm2. These findings demonstrate that the device has a high capture rate in the range of a few fm. The pore size is ≈10 nm in diameter and provides an amplification of the electromagnetic field exceeding 103 in intensity at 785 nm. Owing to these features, single‐molecule detection is achieved by means of surface‐enhanced Raman scattering from a solution containing 50 fm DNA molecules (≈4.4 kilobase pairs). Notably, the reported spectra show an average number of 2.5 Raman counts per nucleotide. From this perspective, this number is not far from what is necessary to discriminate the DNA sequence.

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Artificial Intelligence‐Assisted Label‐Free Spectroscopic Quantification of Global DNA Cytosine Methylation in a Miniature Plasmonic Pickering Emulsion

Xie,

Chen,

Liu

et al. 2023

Adv Funct Materials

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Epigenetic DNA methylations are early and frequently observed events in a diversity of diseases such as cancer. Despite the considerable clinical values for cancer liquid biopsy, quantitative analysis of DNA methylations remains a major challenge due to the lack of rapid, sensitive detection techniques. Here, an artificial intelligence‐assisted label‐free surface‐enhanced Raman spectroscopy (SERS) (iMeSERS) biosensor is reported for simultaneous quantification of C5‐methylcytosine (5mC) level and methylation ratio in DNA samples. This method utilizes the plasmonic Pickering emulsions as the biosensing platform for label‐free SERS detection, formed upon the addition of a sub‐microliter DNA sample to the hydrophobic Au nanostar‐containing n‐decane. Distinct spectral signatures of monophosphates of canonical deoxyribonucleotides (dNMPs) and the common methylation modification 5‐methyl‐2′‐deoxycytidine (d5mCMP) are identified and distinguished by the iMeSERS biosensor. The deep learning algorithms trained with SERS signatures of dNMPs and d5mCMP are then applied to the quantitative analysis of global DNA methylation. The exceptional capability of the deep learning‐driven approach is demonstrated for simultaneous quantification of the methylation ratio and level using a sub‐microliter volume of DNA samples. This work shows the power of label‐free SERS techniques combined with deep learning algorithms for quantitative analysis of epigenetic DNA modifications with great promises for clinical diagnosis.

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Surface-Enhanced Hyper-Raman Spectra of Adenine, Guanine, Cytosine, Thymine, and Uracil

Cited by 158 publications

References 46 publications

Surface enhanced hyper Raman scattering (SEHRS) and its applications

Surface enhanced hyper Raman scattering (SEHRS) and its applications

High‐Density Plasmonic Nanopores for DNA Sensing at Ultra‐Low Concentrations by Plasmon‐Enhanced Raman Spectroscopy

Artificial Intelligence‐Assisted Label‐Free Spectroscopic Quantification of Global DNA Cytosine Methylation in a Miniature Plasmonic Pickering Emulsion

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