This paper describes
an application of attenuated total reflection
Fourier transform infrared spectroscopy (ATR-FTIR) and surface-enhanced
infrared spectroscopy (SEIRA) to characterize the selective adsorption
of four peptides present in body fluids such as neuromedin B (NMB),
bombesin (BN), neurotensin (NT), and bradykinin (BK), which are known
as markers for various human carcinomas. To perform a reliable analysis
of the SERIA spectra of these peptides, curve fitting of these spectra
in the spectral region above 1500 cm
–1
and SEIRA
measurements of sulfur-containing and aromatic amino acids were performed.
On the basis of the analyses of the spectral profiles, specific conclusions
were drawn regarding specific molecule–metal interactions and
changes in the interaction during the substrate change from the surface
of silver nanoparticles (AgNPs) to gold nanoparticles (AuNPs).