Surface engineering of silica nanoparticles for oral insulin delivery: Characterization and cell toxicity studies

Andreani, Tatiana; Kiill, Charlene P.; Souza, Ana Luiza Ribeiro de; Fangueiro, Joana F.; Fernandes, Lisete; Doktorovová, Slavomíra; Santos, Dario L.; García, María L.; Gremião, Maria Palmira Daflon; Souto, Eliana B.; Silva, Amélia M.

doi:10.1016/j.colsurfb.2014.10.047

Cited by 99 publications

(68 citation statements)

References 44 publications

(1 reference statement)

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“…Test solutions were prepared just prior to cell application, by dispersing the required volume of particles in FBS-free culture media to achieve the desired final concentration. Test solutions (100 µL/well; in quadruplicates) were added to the cells, after removing the growing culture media, and cells were exposed for 24 h or 48 h to test solutions before the cell viability assessment using the Alamar Blue (Invitrogen; Alfagene, Lisbon, Portugal), as previously reported [27,28]. Briefly, 24 h or 48 h after cell exposure, the incubating test solutions were removed and replaced by Alamar Blue solution (10% (v/v) in FBS-free culture media; 100 µL/well).…”

Section: Cell Culture and Viability Assaymentioning

confidence: 99%

“…The absorbances, at 570 and 620 nm (reduced and oxidative form or resazurin, respectively [22]) were read 5 h after the addition of Alamar Blue solution. Data were analyzed by calculating the percentage of Alamar blue reduction (using the equations and procedures as previously reported [4,26,27]) and results were expressed as percentage of control (untreated cells).…”

Section: Cell Culture and Viability Assaymentioning

confidence: 99%

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Soft Cationic Nanoparticles for Drug Delivery: Production and Cytotoxicity of Solid Lipid Nanoparticles (SLNs)

et al. 2019

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The surface properties of nanoparticles have decisive influence on their interaction with biological barriers (i.e., living cells), being the concentration and type of surfactant factors to have into account. As a result of different molecular structure, charge, and degree of lipophilicity, different surfactants may interact differently with the cell membrane exhibiting different degrees of cytotoxicity. In this work, the cytotoxicity of two cationic solid lipid nanoparticles (SLNs), differing in the cationic lipids used as surfactants CTAB (cetyltrimethylammonium bromide) or DDAB (dimethyldioctadecylammonium bromide), referred as CTAB-SLNs and DDAB-SLNs, respectively, was assessed against five different human cell lines (Caco-2, HepG2, MCF-7, SV-80, and Y-79). Results showed that the cationic lipids used in SLN production highly influenced the cytotoxic profile of the particles, with CTAB-SLNs being highly cytotoxic even at low concentrations (IC50 < 10 µg/mL, expressed as CTAB amount). DDAB-SLNs produced much lower cytotoxicity, even at longer exposure time (IC50 from 284.06 ± 17.01 µg/mL (SV-80) to 869.88 ± 62.45 µg/mL (MCF-7), at 48 h). To the best of our knowledge, this is the first report that compares the cytotoxic profile of CTAB-SLNs and DDAB-SLNs based on the concentration and time of exposure, using different cell lines. In conclusion, the choice of the right surfactant for biological applications influences the biocompatibility of the nanoparticles. Regardless the type of drug delivery system, not only the cytotoxicity of the drug-loaded nanoparticles should be assessed, but also the blank (non-loaded) nanoparticles as their surface properties play a decisive role both in vitro and in vivo.

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Section: Cell Culture and Viability Assaymentioning

confidence: 99%

Section: Cell Culture and Viability Assaymentioning

confidence: 99%

Soft Cationic Nanoparticles for Drug Delivery: Production and Cytotoxicity of Solid Lipid Nanoparticles (SLNs)

et al. 2019

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“…All reagents and consumables for cell culture were obtained from Gibco (Alfagene, Lisbon, Portugal). The general methods applied for handling these cell lines are already described in the literature [51,52].…”

Section: In Vitro Photobiological Evaluationmentioning

confidence: 99%

“…After about 4 to 5 h of Alamar Blue incubation, absorbance at 570 (reduced form) and 620 nm (oxidized form) was read in a Multiskan EX microplate reader (MTX Labsystems, Vantaa, Finland). The data were analyzed by calculating the percentage of Alamar Blue reduction and expressed as a percentage of control (untreated cells), as previously described [51].…”

Section: In Vitro Photobiological Evaluationmentioning

confidence: 99%

Photophysicochemical Properties and In Vitro Phototherapeutic Effects of Iodoquinoline- and Benzothiazole-Derived Unsymmetrical Squaraine Cyanine Dyes

et al. 2019

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The search to replace conventional cancer treatment therapies, such as chemotherapy, radiotherapy and surgery has led over the last ten years, to a substantial effort in the development of several classes of photodynamic therapy photosensitizers with desired photophysicochemical and photobiological properties.Herein we report the synthesis of 6-iodoquinoline-and benzothiazole-based unsymmetrical squaraine cyanine dyes functionalized with amine groups located in the four-membered central ring. Their photodegradation and singlet oxygen production ability, as well as their in vitro photocytotoxicity against Caco-2 and HepG2 cell lines using a 630.8 ± 0.8 nm centered light-emitting diode system, were also investigated. All photosensitizer candidates displayed strong absorption within the tissue transparency spectral region (650-850 nm). The synthesized dyes were found to have moderate light stability. The potential of these compounds is evidenced by their cytotoxic activity against both tumor cell lines, highlighting the zwitterionic unsubstituted dye, which showed more intense photodynamic activity. Although the singlet oxygen quantum yields of these iodinated derivatives are considered low, it could be concluded that their introduction into the quinoline heterocycle was highly advantageous as it played a role in increasing selective cytotoxicity in the presence of light. Thus, the novel synthesized dyes present photophysicochemical and in vitro photobiological properties that make them excellent photosensitizer candidates for photodynamic therapy.

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“…[42]. After centrifugation and wash with water for several times, the white SiO 2 nanoparticles were heated to 80°C in a vacuum drying oven for 24 h to remove physically absorbed water.…”

Section: Fabrication Of Sio 2 -Nh 2 and Glass-nhmentioning

confidence: 99%

Fluorescent silica nanoparticles and glass surfaces for the detection and removal of Pd(II) ions

2016

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Fluorescent chemosensors based on silica nanoparticles and silicate glass slides were well designed and prepared. The as-prepared fluorescent silica particles and glass slides exhibited highly selective sensing and absorbing abilities to Pd 2? ions in aqueous phase. SiO 2 nanoparticles were first synthesized by the hydrolysis of tetraethyl orthosilicate (TEOS), and then -NH 2 groups were introduced to silica by treating with 3-aminopropyltriethoxysilane (APTES). Subsequently, 4-amino-1,8-naphthalic anhydride (ANA) was incorporated with the reaction of -NH 2 groups. Finally, salicylaldehyde (SA) was introduced by the reaction between amino groups in ANA and the -CHO groups in SA. Thus, the fluorescent silica nanoparticles were obtained. This method was expanded to prepare fluorescent silicate glass slides. Upon the addition of Pd 2? ions, the fluorescence quenched immediately. These chemosensors were selectively sensitive to Pd 2? ions as low as 10 lM. The absorption efficiency of fluorescent nanoparticles and glass slides for Pd 2? reached to 38.38 and 27.50 %, respectively. And the chemosensors also could be easily recycled and reused for at least five times. Fluorescent silica nanoparticles with only ANA or SA (not ANA and SA together) also exhibited the sensing ability to Pd 2? ions; however, it showed the shortage of Pd 2? ions selectivity. It suggested that it is necessary to introduce both ANA and SA for the high sensitivity and selectivity to Pd 2? ions.

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Surface engineering of silica nanoparticles for oral insulin delivery: Characterization and cell toxicity studies

Cited by 99 publications

References 44 publications

Soft Cationic Nanoparticles for Drug Delivery: Production and Cytotoxicity of Solid Lipid Nanoparticles (SLNs)

Soft Cationic Nanoparticles for Drug Delivery: Production and Cytotoxicity of Solid Lipid Nanoparticles (SLNs)

Photophysicochemical Properties and In Vitro Phototherapeutic Effects of Iodoquinoline- and Benzothiazole-Derived Unsymmetrical Squaraine Cyanine Dyes

Fluorescent silica nanoparticles and glass surfaces for the detection and removal of Pd(II) ions

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