Surface engineering of macrophages with nucleic acid aptamers for the capture of circulating tumor cells

Abstract

In order to enhance the interactions between macrophages and cancer cells, thiol-terminated nucleic acid aptamers were immobilized on methacryloyl-functionalised carbohydrates of macrophages. The adhesion of cancer cells on the surface modified macrophages was significantly accelerated.

Cell membrane engineering with synthetic materials: Applications in cell spheroids, cellular glues and microtissue formation

“…Remodeling of the cell membrane with functional biomaterials has only recently emerged and is likely to impact emerging technologies that can be used for therapeutic and diagnostic purposes, i.e. in disease 2/3D in vitro modeling, cell therapeutics and precision medicine [179][180][181][182], cell sorting and cryopreservation technologies [183,184]. In parallel, it is necessary to thoroughly study the long-term effect of cell surface- From the previous discussion, it is emerging that spheroid models with tailored characteristics can be used to direct tissue fusion and potentiate the development of living functional prototissues, for example, with the use of 3D bioprinting and other microfabrication methodologies.…”

Chemically Engineered Immune Cell‐Derived Microrobots and Biomimetic Nanoparticles: Emerging Biodiagnostic and Therapeutic Tools

“…It was observed that engineered M ϕ s’ attachment to target cells increased by extending the incubation time. [ 92 ] The engineered M ϕ s were then co‐incubated with lymphoblasts, being pretreated with DOX for apoptosis induction, and it was revealed that they could capture both dead and alive lymphoblasts. Analyzing the inflammatory response by engineered M ϕ s, it was observed that surface decoration with the aptamers did not activate the M ϕ s and no alteration in the level of cytokine secretion was demonstrated.…”

Programming Cell-Cell Interactions through Non-genetic Membrane Engineering

“…In addition to azido sugar moieties, a synthetic methacryloyl-modified analog of N -acetyl mannosamine (ManNAc), termed ManM, was used to install methacryloyl groups in surface sialic acid residues (Sugimoto et al, 2015). The ManM groups were then coupled to thiol-terminated aptamers targeting protein tyrosine kinase-7 (PTK7) via a light-assisted (505 nm) thiol-ene reaction utilizing eosin Y as a photosensitizer.…”

“…Finally, metabolically functionalized murine macrophage-like cells (RAW264.7) have been labeled with aptamers targeting the circulating cancer cell marker PTK7. These aptamer-targeted macrophages were able to recognize and phagocytose PTK7+ CCRF-CEM (T lymphoblast) cells in vitro (Sugimoto et al, 2015). …”